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Characterization and expression profiles of cyp19a1a in the schizothoracine fish Schizothorax prenanti.

Abstract

Aromatase plays a central role in ovarian differentiation and development in teleosts. In the present study, we identified a cyp19a1a homologue from the ovary of Schizothorax prenanti and analysed its expression at both the mRNA and protein levels. Cyp19a1a of S. prenanti showed high homology with that of other teleosts, especially S. kozlovi. The ovary and testis were the main sites of cyp19a1a expression in S. prenanti, and cyp19a1a transcript levels peaked in the mid-vitellogenic (MVG)-stage ovary and the mid-spermatogenic (MS)-stage testis. Signals of Cyp19a1a immunopositivity were detected in the spermatocytes and follicular cells of cortical alveolar-stage and MVG oocytes but not in spermatogonia or spermatids. Taken together, these findings indicate that Cyp19a1a may play an important role in oocyte vitellogenesis as well as spermatocyte development in S. prenanti.

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  • Authors+Show Affiliations

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    Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China.

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    Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China.

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    Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China.

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    Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China.

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    Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China.

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    Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China.

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    Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China.

    Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China. Electronic address: zhihe@sicau.edu.cn.

    Source

    Tissue & cell 58: 2019 Jun pg 70-75

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31133248

    Citation

    Yan, Taiming, et al. "Characterization and Expression Profiles of Cyp19a1a in the Schizothoracine Fish Schizothorax Prenanti." Tissue & Cell, vol. 58, 2019, pp. 70-75.
    Yan T, Cai Y, He J, et al. Characterization and expression profiles of cyp19a1a in the schizothoracine fish Schizothorax prenanti. Tissue Cell. 2019;58:70-75.
    Yan, T., Cai, Y., He, J., Zhang, Q., Wang, X., Zhang, S., ... He, Z. (2019). Characterization and expression profiles of cyp19a1a in the schizothoracine fish Schizothorax prenanti. Tissue & Cell, 58, pp. 70-75. doi:10.1016/j.tice.2019.04.008.
    Yan T, et al. Characterization and Expression Profiles of Cyp19a1a in the Schizothoracine Fish Schizothorax Prenanti. Tissue Cell. 2019;58:70-75. PubMed PMID: 31133248.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Characterization and expression profiles of cyp19a1a in the schizothoracine fish Schizothorax prenanti. AU - Yan,Taiming, AU - Cai,Yueping, AU - He,Jiayang, AU - Zhang,Qian, AU - Wang,Xiongyan, AU - Zhang,Songpei, AU - He,Liang, AU - He,Zhi, Y1 - 2019/04/22/ PY - 2019/01/18/received PY - 2019/04/11/revised PY - 2019/04/19/accepted PY - 2019/5/29/entrez PY - 2019/5/28/pubmed PY - 2019/5/28/medline KW - Cyp19a1a KW - Expression KW - Localization KW - Schizothorax prenanti SP - 70 EP - 75 JF - Tissue & cell JO - Tissue Cell VL - 58 N2 - Aromatase plays a central role in ovarian differentiation and development in teleosts. In the present study, we identified a cyp19a1a homologue from the ovary of Schizothorax prenanti and analysed its expression at both the mRNA and protein levels. Cyp19a1a of S. prenanti showed high homology with that of other teleosts, especially S. kozlovi. The ovary and testis were the main sites of cyp19a1a expression in S. prenanti, and cyp19a1a transcript levels peaked in the mid-vitellogenic (MVG)-stage ovary and the mid-spermatogenic (MS)-stage testis. Signals of Cyp19a1a immunopositivity were detected in the spermatocytes and follicular cells of cortical alveolar-stage and MVG oocytes but not in spermatogonia or spermatids. Taken together, these findings indicate that Cyp19a1a may play an important role in oocyte vitellogenesis as well as spermatocyte development in S. prenanti. SN - 1532-3072 UR - https://www.unboundmedicine.com/medline/citation/31133248/Characterization_and_expression_profiles_of_cyp19a1a_in_the_schizothoracine_fish_Schizothorax_prenanti L2 - https://linkinghub.elsevier.com/retrieve/pii/S0040-8166(19)30022-9 DB - PRIME DP - Unbound Medicine ER -