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Faecal Scent as a Novel Non-Invasive Biomarker to Discriminate between Coeliac Disease and Refractory Coeliac Disease: A Proof of Principle Study.
Biosensors (Basel) 2019; 9(2)B

Abstract

Currently, the gold standard for diagnosis of coeliac disease (CD) is based on serology and gastroduodenoscopy with histology of duodenal mucosal biopsies. The aim of this study was to evaluate the potential of faecal volatile organic compounds (VOCs) analysis as a novel, non-invasive tool to discriminate between CD in remission in patients on a gluten-free diet (GFD), refractory coeliac disease (RCD) and controls without CD. Patients with an established diagnosis of CD on a GFD, RCD and healthy controls (HC) were instructed to collect a faecal sample. All subjects completed questionnaires on clinical symptoms, lifestyle and dietary information. Faecal VOCs were measured using gas chromatography-ion mobility spectrometry. A total of 13 CD, 7 RCD and 10 HC were included. A significant difference in VOC profiles between CD and RCD patients (area under the curve (AUC) ± 95% CI: 0.91 (0.79-1) p = 0.000) and between CD and HC (AUC ± 95% CI: 0.71 (0.51-0.91) p = 0.0254) was observed. We found no significant differences between faecal VOC patterns of HC and RCD. Based on faecal VOCs, CD could be discriminated from RCD and HC. This implies that faecal VOC analysis may hold potential as a novel non-invasive biomarker for RCD. Future studies should encompass a larger cohort to further investigate and validate this prior to application in clinical practice.

Authors+Show Affiliations

Department of Gastroenterology and Hepatology, Vrije Universiteit Amsterdam, Amsterdam UMC, AG&M research institute, 1081 HZ Amsterdam, The Netherlands. m.rouvroye@vumc.nl.School of Engineering, University of Warwick, Coventry CV4 7AL, UK. A.Wicaksono@warwick.ac.uk.Department of Gastroenterology and Hepatology, Vrije Universiteit Amsterdam, Amsterdam UMC, AG&M research institute, 1081 HZ Amsterdam, The Netherlands. s.bosch1@vumc.nl.Department of Gastroenterology and Hepatology, Vrije Universiteit Amsterdam, Amsterdam UMC, AG&M research institute, 1081 HZ Amsterdam, The Netherlands. edosavelkoul@gmail.com.School of Engineering, University of Warwick, Coventry CV4 7AL, UK. J.A.Covington@warwick.ac.uk.Department of Gastroenterology and Hepatology, Vrije Universiteit Amsterdam, Amsterdam UMC, AG&M research institute, 1081 HZ Amsterdam, The Netherlands. h.beaumont@vumc.nl.Department of Gastroenterology and Hepatology, Vrije Universiteit Amsterdam, Amsterdam UMC, AG&M research institute, 1081 HZ Amsterdam, The Netherlands. cjmulder@vumc.nl.Department of Gastroenterology and Hepatology, Vrije Universiteit Amsterdam, Amsterdam UMC, AG&M research institute, 1081 HZ Amsterdam, The Netherlands. g.bouma@vumc.nl.Department of Paediatric Gastroenterology, Vrije Universiteit Amsterdam, Amsterdam UMC, 1081 HZ Amsterdam, The Netherlands. t.demeij@amsterdamumc.nl.Department of Gastroenterology and Hepatology, Vrije Universiteit Amsterdam, Amsterdam UMC, AG&M research institute, 1081 HZ Amsterdam, The Netherlands. khn.deboer@vumc.nl.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31137798

Citation

Rouvroye, Maxine D., et al. "Faecal Scent as a Novel Non-Invasive Biomarker to Discriminate Between Coeliac Disease and Refractory Coeliac Disease: a Proof of Principle Study." Biosensors, vol. 9, no. 2, 2019.
Rouvroye MD, Wicaksono A, Bosch S, et al. Faecal Scent as a Novel Non-Invasive Biomarker to Discriminate between Coeliac Disease and Refractory Coeliac Disease: A Proof of Principle Study. Biosensors (Basel). 2019;9(2).
Rouvroye, M. D., Wicaksono, A., Bosch, S., Savelkoul, E., Covington, J. A., Beaumont, H., ... de Boer, N. K. H. (2019). Faecal Scent as a Novel Non-Invasive Biomarker to Discriminate between Coeliac Disease and Refractory Coeliac Disease: A Proof of Principle Study. Biosensors, 9(2), doi:10.3390/bios9020069.
Rouvroye MD, et al. Faecal Scent as a Novel Non-Invasive Biomarker to Discriminate Between Coeliac Disease and Refractory Coeliac Disease: a Proof of Principle Study. Biosensors (Basel). 2019 May 27;9(2) PubMed PMID: 31137798.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Faecal Scent as a Novel Non-Invasive Biomarker to Discriminate between Coeliac Disease and Refractory Coeliac Disease: A Proof of Principle Study. AU - Rouvroye,Maxine D, AU - Wicaksono,Alfian, AU - Bosch,Sofie, AU - Savelkoul,Edo, AU - Covington,James A, AU - Beaumont,Hanneke, AU - Mulder,Chris J, AU - Bouma,Gerd, AU - de Meij,Tim G J, AU - de Boer,Nanne K H, Y1 - 2019/05/27/ PY - 2019/04/26/received PY - 2019/05/22/revised PY - 2019/05/23/accepted PY - 2019/5/30/entrez PY - 2019/5/30/pubmed PY - 2019/5/30/medline KW - biomarker KW - celiac disease KW - electronic nose KW - non-invasive diagnostics KW - refractory celiac disease KW - volatile organic compounds JF - Biosensors JO - Biosensors (Basel) VL - 9 IS - 2 N2 - Currently, the gold standard for diagnosis of coeliac disease (CD) is based on serology and gastroduodenoscopy with histology of duodenal mucosal biopsies. The aim of this study was to evaluate the potential of faecal volatile organic compounds (VOCs) analysis as a novel, non-invasive tool to discriminate between CD in remission in patients on a gluten-free diet (GFD), refractory coeliac disease (RCD) and controls without CD. Patients with an established diagnosis of CD on a GFD, RCD and healthy controls (HC) were instructed to collect a faecal sample. All subjects completed questionnaires on clinical symptoms, lifestyle and dietary information. Faecal VOCs were measured using gas chromatography-ion mobility spectrometry. A total of 13 CD, 7 RCD and 10 HC were included. A significant difference in VOC profiles between CD and RCD patients (area under the curve (AUC) ± 95% CI: 0.91 (0.79-1) p = 0.000) and between CD and HC (AUC ± 95% CI: 0.71 (0.51-0.91) p = 0.0254) was observed. We found no significant differences between faecal VOC patterns of HC and RCD. Based on faecal VOCs, CD could be discriminated from RCD and HC. This implies that faecal VOC analysis may hold potential as a novel non-invasive biomarker for RCD. Future studies should encompass a larger cohort to further investigate and validate this prior to application in clinical practice. SN - 2079-6374 UR - https://www.unboundmedicine.com/medline/citation/31137798/Faecal_Scent_as_a_Novel_Non-Invasive_Biomarker_to_Discriminate_between_Coeliac_Disease_and_Refractory_Coeliac_Disease:_A_Proof_of_Principle_Study L2 - http://www.mdpi.com/resolver?pii=bios9020069 DB - PRIME DP - Unbound Medicine ER -