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Boosting the Sensitivity and Selectivity of a Miniature Mass Spectrometer Using a Hybrid Ion Funnel.

Abstract

Besides high portability, high analytical performances are also crucial concerns for a miniature mass spectrometer to meet the demands in in situ analysis. As a continuous effort in improving analytical performances of the miniature mass spectrometer with continuous atmospheric pressure interface, a hybrid ion funnel was developed and coupled into the system in this study. The hybrid ion funnel consisted of a rectangular ion funnel region and a planar quadrupole field region, which were fabricated by the printed circuit board technology. After systematic optimization, a limit of detection of 1 ng/mL was obtained, which was improved by 10 folds relative to that of 10 ng/mL previously reported for the miniature mass spectrometer. Besides improved ion transmission efficiency, this hybrid ion funnel was also capable of filtering ions according to their mobilities, thus improving the system selectivity. This capability was demonstrated by separation and selective transmission of protein ions at different charge states, reserpine in PEG background and isobaric peptide ions. Resolution of this system was also tested by analyzing isotopic peaks of reserpine. The ppb-level detection sensitivity and isotope resolving capability achieved in this work would greatly expand the application range of miniature mass spectrometers.

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  • Authors+Show Affiliations

    ,

    School of Life Science , Beijing Institute of Technology , Beijing 100081 , China.

    ,

    School of Life Science , Beijing Institute of Technology , Beijing 100081 , China.

    ,

    School of Life Science , Beijing Institute of Technology , Beijing 100081 , China.

    ,

    School of Life Science , Beijing Institute of Technology , Beijing 100081 , China.

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    School of Computer Science and Engineering , Northeastern University , Shenyang , 110819 , China.

    School of Life Science , Beijing Institute of Technology , Beijing 100081 , China.

    Source

    Analytical chemistry 91:12 2019 Jun 18 pg 7911-7919

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31137927

    Citation

    Zhai, Yanbing, et al. "Boosting the Sensitivity and Selectivity of a Miniature Mass Spectrometer Using a Hybrid Ion Funnel." Analytical Chemistry, vol. 91, no. 12, 2019, pp. 7911-7919.
    Zhai Y, Xu Q, Tang Y, et al. Boosting the Sensitivity and Selectivity of a Miniature Mass Spectrometer Using a Hybrid Ion Funnel. Anal Chem. 2019;91(12):7911-7919.
    Zhai, Y., Xu, Q., Tang, Y., Liu, S., Li, D., & Xu, W. (2019). Boosting the Sensitivity and Selectivity of a Miniature Mass Spectrometer Using a Hybrid Ion Funnel. Analytical Chemistry, 91(12), pp. 7911-7919. doi:10.1021/acs.analchem.9b01770.
    Zhai Y, et al. Boosting the Sensitivity and Selectivity of a Miniature Mass Spectrometer Using a Hybrid Ion Funnel. Anal Chem. 2019 Jun 18;91(12):7911-7919. PubMed PMID: 31137927.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Boosting the Sensitivity and Selectivity of a Miniature Mass Spectrometer Using a Hybrid Ion Funnel. AU - Zhai,Yanbing, AU - Xu,Qian, AU - Tang,Yang, AU - Liu,Siyu, AU - Li,Dayu, AU - Xu,Wei, Y1 - 2019/06/07/ PY - 2019/5/30/pubmed PY - 2019/5/30/medline PY - 2019/5/30/entrez SP - 7911 EP - 7919 JF - Analytical chemistry JO - Anal. Chem. VL - 91 IS - 12 N2 - Besides high portability, high analytical performances are also crucial concerns for a miniature mass spectrometer to meet the demands in in situ analysis. As a continuous effort in improving analytical performances of the miniature mass spectrometer with continuous atmospheric pressure interface, a hybrid ion funnel was developed and coupled into the system in this study. The hybrid ion funnel consisted of a rectangular ion funnel region and a planar quadrupole field region, which were fabricated by the printed circuit board technology. After systematic optimization, a limit of detection of 1 ng/mL was obtained, which was improved by 10 folds relative to that of 10 ng/mL previously reported for the miniature mass spectrometer. Besides improved ion transmission efficiency, this hybrid ion funnel was also capable of filtering ions according to their mobilities, thus improving the system selectivity. This capability was demonstrated by separation and selective transmission of protein ions at different charge states, reserpine in PEG background and isobaric peptide ions. Resolution of this system was also tested by analyzing isotopic peaks of reserpine. The ppb-level detection sensitivity and isotope resolving capability achieved in this work would greatly expand the application range of miniature mass spectrometers. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/31137927/Boosting_the_Sensitivity_and_Selectivity_of_a_Miniature_Mass_Spectrometer_Using_a_Hybrid_Ion_Funnel L2 - https://dx.doi.org/10.1021/acs.analchem.9b01770 DB - PRIME DP - Unbound Medicine ER -