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A Novel Liquid Chromatography Tandem Mass Spectrometry Technique using Multi-Period-Multi-Experiment of MRM-EPI-MRM3 with Library Matching for Simultaneous Determination of Amphetamine Type Stimulants Related Drugs in Whole Blood, Urine and Dried Blood Stain (DBS)-Application to Forensic Toxicology Cases in Malaysia.

Abstract

A novel mass spectrometry detection technique based on a multi-period and multi- experiment (MRM-EPI-MRM3) with library matching in a single run for fast and rapid screening and identification of amphetamine type stimulants (ATS) related drugs in whole blood, urine and dried blood stain was developed and validated. The ATS-related drugs analyzed in this study include ephedrine, pseudoephedrine, amphetamine, methamphetamine, MDMA (3,4-Methylenedioxymethamphetamine), MDA (3,4-Methylenedioxyamphetamine), MDEA (3,4-Methylenedioxy-N-ethylamphetamine) and phentermine. The relative standard deviation for inter and intraday was less than 15% while recoveries ranged from 80% to 120% for all three matrices, i.e., whole blood, urine and dried blood stain. All compounds gave library matching percentage of more than 85% based on the purity. This method was proven to be simple and robust, and provide high confident results complemented with library matching confirmation.

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  • Authors+Show Affiliations

    ,

    Department of Chemistry, University of Malaya, Kuala Lumpur, Malaysia. Department of Chemistry Malaysia, Ministry of Science, Technology and Innovation (MOSTI), Petaling Jaya, Malaysia.

    ,

    Department of Chemistry, University of Malaya, Kuala Lumpur, Malaysia.

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    Department of Chemistry, University of Malaya, Kuala Lumpur, Malaysia.

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    Department of Chemistry Malaysia, Ministry of Science, Technology and Innovation (MOSTI), Petaling Jaya, Malaysia.

    Department of Chemistry Malaysia, Ministry of Science, Technology and Innovation (MOSTI), Petaling Jaya, Malaysia.

    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31141150

    Citation

    Zubaidi, Fathiah A., et al. "A Novel Liquid Chromatography Tandem Mass Spectrometry Technique Using Multi-Period-Multi-Experiment of MRM-EPI-MRM3 With Library Matching for Simultaneous Determination of Amphetamine Type Stimulants Related Drugs in Whole Blood, Urine and Dried Blood Stain (DBS)-Application to Forensic Toxicology Cases in Malaysia." Journal of Analytical Toxicology, 2019.
    Zubaidi FA, Choo YM, Tan GH, et al. A Novel Liquid Chromatography Tandem Mass Spectrometry Technique using Multi-Period-Multi-Experiment of MRM-EPI-MRM3 with Library Matching for Simultaneous Determination of Amphetamine Type Stimulants Related Drugs in Whole Blood, Urine and Dried Blood Stain (DBS)-Application to Forensic Toxicology Cases in Malaysia. J Anal Toxicol. 2019.
    Zubaidi, F. A., Choo, Y. M., Tan, G. H., Hamid, H. A., & Choy, Y. K. (2019). A Novel Liquid Chromatography Tandem Mass Spectrometry Technique using Multi-Period-Multi-Experiment of MRM-EPI-MRM3 with Library Matching for Simultaneous Determination of Amphetamine Type Stimulants Related Drugs in Whole Blood, Urine and Dried Blood Stain (DBS)-Application to Forensic Toxicology Cases in Malaysia. Journal of Analytical Toxicology, doi:10.1093/jat/bkz017.
    Zubaidi FA, et al. A Novel Liquid Chromatography Tandem Mass Spectrometry Technique Using Multi-Period-Multi-Experiment of MRM-EPI-MRM3 With Library Matching for Simultaneous Determination of Amphetamine Type Stimulants Related Drugs in Whole Blood, Urine and Dried Blood Stain (DBS)-Application to Forensic Toxicology Cases in Malaysia. J Anal Toxicol. 2019 May 29; PubMed PMID: 31141150.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - A Novel Liquid Chromatography Tandem Mass Spectrometry Technique using Multi-Period-Multi-Experiment of MRM-EPI-MRM3 with Library Matching for Simultaneous Determination of Amphetamine Type Stimulants Related Drugs in Whole Blood, Urine and Dried Blood Stain (DBS)-Application to Forensic Toxicology Cases in Malaysia. AU - Zubaidi,Fathiah A, AU - Choo,Yeun-Mun, AU - Tan,Guan-Huat, AU - Hamid,Hamimah Abd, AU - Choy,Yap Ken, Y1 - 2019/05/29/ PY - 2018/07/30/received PY - 2018/11/21/revised PY - 2019/02/27/accepted PY - 2019/5/30/entrez KW - ATS-related drugs KW - LC-MS/MS KW - MRM-EPI-MRM3 KW - dried blood stain sample KW - urine sample KW - whole blood sample JF - Journal of analytical toxicology JO - J Anal Toxicol N2 - A novel mass spectrometry detection technique based on a multi-period and multi- experiment (MRM-EPI-MRM3) with library matching in a single run for fast and rapid screening and identification of amphetamine type stimulants (ATS) related drugs in whole blood, urine and dried blood stain was developed and validated. The ATS-related drugs analyzed in this study include ephedrine, pseudoephedrine, amphetamine, methamphetamine, MDMA (3,4-Methylenedioxymethamphetamine), MDA (3,4-Methylenedioxyamphetamine), MDEA (3,4-Methylenedioxy-N-ethylamphetamine) and phentermine. The relative standard deviation for inter and intraday was less than 15% while recoveries ranged from 80% to 120% for all three matrices, i.e., whole blood, urine and dried blood stain. All compounds gave library matching percentage of more than 85% based on the purity. This method was proven to be simple and robust, and provide high confident results complemented with library matching confirmation. SN - 1945-2403 UR - https://www.unboundmedicine.com/medline/citation/31141150/A_Novel_Liquid_Chromatography_Tandem_Mass_Spectrometry_Technique_using_Multi-Period-Multi-Experiment_of_MRM-EPI-MRM3_with_Library_Matching_for_Simultaneous_Determination_of_Amphetamine_Type_Stimulants_Related_Drugs_in_Whole_Blood,_Urine_and_Dried_Blood_Stain_(DBS)-Application_to_Forensic_Toxicology_Cases_in_Malaysia L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/bkz017 DB - PRIME DP - Unbound Medicine ER -