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Ultrastructure of spermiogenesis and the distribution of spermatozoal nuclear histones in the Japanese mantis shrimp, Oratosquilla oratoria (Crustacea: Stomatopoda).

Abstract

The Japanese mantis shrimp Oratosquilla oratoria (Stomatopoda; Crustacea) is one of the most economically important aquatic species of Pacific shrimp and it is distributed from Japan to the coast of China, the Philippines, the Malay Peninsula, and the Hawaiian Islands. Early studies described certain characteristics of spermatogenesis and the sperm ultrastructure in Stomatopoda, but the composition of sperm basic nuclear proteins (SBNPs) remains completely unknown. We studied the sperm ultrastructure of O. oratoria using transmission electron microscopy and the histone composition using immunofluorescence and immunoelectron microscopy. We found that the spherical nucleus is adjacent to the electron translucent external coat, which occurs in early spermatids. The acrosomal structure begins to form at the junction of the nucleus and the external coat. At the mid-spermatid stage, part of the chromatin appears to be more electron-dense than the external coat side. The aflagellate sperm of O. oratoria, are rounded or slightly ovoid in shape and have a consistent granular nucleus, an acrosome structure of pushpin shape and a spherical vesicular body in which faintly granular material is scattered. The acrosome consists of an acrosomal vesicle, perforatorium, and subacrosomal material. The sperm contains histones H2A, H2B, H3, H4, H3.3, H2AX, and H2AZ as well as some histone modifications, that is, H3K9me3, H3K4me2, H3S10ph, H4Kac, and H2A + H4S1ph. Histones are localized not only in the nucleus of the sperm but also in other structures outside the nucleus. The results may provide new perspectives for systematic studies of crustaceans and their sperm chromatin components. These findings extend the study of the sperm structure of Stomatopoda and provide basic data to elucidate the epigenetic mechanism of fertilization.

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  • Authors+Show Affiliations

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    College of Life Sciences, Hebei University, Baoding, Hebei, China.

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    College of Life Sciences, Hebei University, Baoding, Hebei, China.

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    College of Life Sciences, Hebei University, Baoding, Hebei, China.

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    College of Life Sciences, Hebei University, Baoding, Hebei, China.

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    College of Life Sciences, Hebei University, Baoding, Hebei, China.

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    College of Life Sciences, Hebei University, Baoding, Hebei, China.

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    College of Life Sciences, Hebei University, Baoding, Hebei, China.

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    Department of Reproductive Medicine, Baoding No. 1 Central Hospital, Baoding, Hebei, China.

    College of Life Sciences, Hebei University, Baoding, Hebei, China.

    Source

    Journal of morphology 280:8 2019 Aug pg 1170-1184

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31141207

    Citation

    Chen, Tingrong, et al. "Ultrastructure of Spermiogenesis and the Distribution of Spermatozoal Nuclear Histones in the Japanese Mantis Shrimp, Oratosquilla Oratoria (Crustacea: Stomatopoda)." Journal of Morphology, vol. 280, no. 8, 2019, pp. 1170-1184.
    Chen T, Sun Z, Mu S, et al. Ultrastructure of spermiogenesis and the distribution of spermatozoal nuclear histones in the Japanese mantis shrimp, Oratosquilla oratoria (Crustacea: Stomatopoda). J Morphol. 2019;280(8):1170-1184.
    Chen, T., Sun, Z., Mu, S., Jiang, L., Li, C., Li, L., ... Kang, X. (2019). Ultrastructure of spermiogenesis and the distribution of spermatozoal nuclear histones in the Japanese mantis shrimp, Oratosquilla oratoria (Crustacea: Stomatopoda). Journal of Morphology, 280(8), pp. 1170-1184. doi:10.1002/jmor.21008.
    Chen T, et al. Ultrastructure of Spermiogenesis and the Distribution of Spermatozoal Nuclear Histones in the Japanese Mantis Shrimp, Oratosquilla Oratoria (Crustacea: Stomatopoda). J Morphol. 2019;280(8):1170-1184. PubMed PMID: 31141207.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Ultrastructure of spermiogenesis and the distribution of spermatozoal nuclear histones in the Japanese mantis shrimp, Oratosquilla oratoria (Crustacea: Stomatopoda). AU - Chen,Tingrong, AU - Sun,Zhe, AU - Mu,Shumei, AU - Jiang,Lingling, AU - Li,Chao, AU - Li,Lu, AU - Guo,Mingshen, AU - Zhang,Zhaohui, AU - Kang,Xianjiang, Y1 - 2019/05/29/ PY - 2019/04/01/received PY - 2019/05/11/revised PY - 2019/05/14/accepted PY - 2019/5/30/pubmed PY - 2019/5/30/medline PY - 2019/5/30/entrez KW - Stomatopoda KW - decondensed nuclei KW - histone KW - sperm SP - 1170 EP - 1184 JF - Journal of morphology JO - J. Morphol. VL - 280 IS - 8 N2 - The Japanese mantis shrimp Oratosquilla oratoria (Stomatopoda; Crustacea) is one of the most economically important aquatic species of Pacific shrimp and it is distributed from Japan to the coast of China, the Philippines, the Malay Peninsula, and the Hawaiian Islands. Early studies described certain characteristics of spermatogenesis and the sperm ultrastructure in Stomatopoda, but the composition of sperm basic nuclear proteins (SBNPs) remains completely unknown. We studied the sperm ultrastructure of O. oratoria using transmission electron microscopy and the histone composition using immunofluorescence and immunoelectron microscopy. We found that the spherical nucleus is adjacent to the electron translucent external coat, which occurs in early spermatids. The acrosomal structure begins to form at the junction of the nucleus and the external coat. At the mid-spermatid stage, part of the chromatin appears to be more electron-dense than the external coat side. The aflagellate sperm of O. oratoria, are rounded or slightly ovoid in shape and have a consistent granular nucleus, an acrosome structure of pushpin shape and a spherical vesicular body in which faintly granular material is scattered. The acrosome consists of an acrosomal vesicle, perforatorium, and subacrosomal material. The sperm contains histones H2A, H2B, H3, H4, H3.3, H2AX, and H2AZ as well as some histone modifications, that is, H3K9me3, H3K4me2, H3S10ph, H4Kac, and H2A + H4S1ph. Histones are localized not only in the nucleus of the sperm but also in other structures outside the nucleus. The results may provide new perspectives for systematic studies of crustaceans and their sperm chromatin components. These findings extend the study of the sperm structure of Stomatopoda and provide basic data to elucidate the epigenetic mechanism of fertilization. SN - 1097-4687 UR - https://www.unboundmedicine.com/medline/citation/31141207/Ultrastructure_of_spermiogenesis_and_the_distribution_of_spermatozoal_nuclear_histones_in_the_Japanese_mantis_shrimp,_Oratosquilla_oratoria_(Crustacea:_Stomatopoda) L2 - https://doi.org/10.1002/jmor.21008 DB - PRIME DP - Unbound Medicine ER -