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Effect of herbal antioxidant-rich formula on improvement of antioxidant defense system and heat shock protein-70 expression in recreational female athletes: A randomized controlled trial.

Abstract

Background

The aim of the present study was to evaluate the effect of natural antioxidant formula (blend of herbs: ginger root, cinnamon bark and raw almond fruit powder, rosemary leaf powder, and honey) on oxidative status, antioxidant enzyme activity, and relative heat shock protein (HSP-70) expression in recreational female athletes.

Materials and Methods

Eighteen female participants trained for 4 weeks and randomly received either antioxidant formula (FormEX) (n = 8) or placebo (PlcEX) (n = 10) in a randomized controlled trial. Blood samples were obtained 1-h before, 1 h and 24 h postexercise to measure malondialdehyde (MDA), total antioxidant capacity (TAC), superoxide dismutase (SOD), glutathione peroxidases (GPx), and HSP70 mRNA expression. Data analysis was performed using 2 (treatment = grouping factor) ×6 (time = within-factor) repeated measurements analysis of variance or generalized estimating equations (GEE) test. We used the independent t-test to evaluate any significant differences for real-time polymerase chain reaction data.

Results

Antioxidant formula increased the relative HSP-70 mRNA expression more than Plc-EX group in all time points (P = 0.001). The time main effect was significant with regard to TAC and SOD concentrations (P = 0.001 and 0.002, respectively). However, there were no statistically significant differences between groups for TAC, SOD, and MDA (P = 0.25, 0.06, and 0.38, respectively). Neither the time main effect for MDA nor time and intervention interaction was not statistically significant for MDA, TAC, and SOD (P = 0.19, 0.13, and 0.10, respectively). GEE results for GPx showed that there were no significant differences between the groups (P = 0.11).

Conclusion

The results presented herein revealed that natural antioxidant rich formula had variable effects on oxidative status. However, in contrast to many antioxidant supplements, this formulation increases the HSP-70 mRNA expression which might improve the antioxidant ability of cells in the long-term period and exercise-induced adaptation.

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  • Authors+Show Affiliations

    ,

    Department of Nutrition, Faculty of Paramedicine, Nutrition and Metabolic Disease Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Department of Nutrition, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran.

    ,

    Department of Clinical Nutrition, School of Nutrition and Food Science, Food Security Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. Faculty of Paramedicine, Diabetes Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

    ,

    Nutrition and Metabolic Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.

    ,

    Diabetes Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Department of Medical Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

    ,

    Department of Biological Sciences in Sport, Faculty of Sport Sciences and Health, Shahid Beheshti University, Tehran, Iran. Department of Exercise Physiology, Faculty of Physical Education & Sport Sciences, University of Tabriz, Tabriz, Iran.

    Department of Biostatistics and Epidemiology, School of Public Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31143238

    Citation

    Daneghian, Sevana, et al. "Effect of Herbal Antioxidant-rich Formula On Improvement of Antioxidant Defense System and Heat Shock Protein-70 Expression in Recreational Female Athletes: a Randomized Controlled Trial." Journal of Research in Medical Sciences : the Official Journal of Isfahan University of Medical Sciences, vol. 24, 2019, p. 37.
    Daneghian S, Amani R, Hosseini SA, et al. Effect of herbal antioxidant-rich formula on improvement of antioxidant defense system and heat shock protein-70 expression in recreational female athletes: A randomized controlled trial. J Res Med Sci. 2019;24:37.
    Daneghian, S., Amani, R., Hosseini, S. A., Ghandil, P., Jafari, A., & Saki Malehi, A. (2019). Effect of herbal antioxidant-rich formula on improvement of antioxidant defense system and heat shock protein-70 expression in recreational female athletes: A randomized controlled trial. Journal of Research in Medical Sciences : the Official Journal of Isfahan University of Medical Sciences, 24, p. 37. doi:10.4103/jrms.JRMS_591_18.
    Daneghian S, et al. Effect of Herbal Antioxidant-rich Formula On Improvement of Antioxidant Defense System and Heat Shock Protein-70 Expression in Recreational Female Athletes: a Randomized Controlled Trial. J Res Med Sci. 2019;24:37. PubMed PMID: 31143238.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Effect of herbal antioxidant-rich formula on improvement of antioxidant defense system and heat shock protein-70 expression in recreational female athletes: A randomized controlled trial. AU - Daneghian,Sevana, AU - Amani,Reza, AU - Hosseini,Seyed Ahmad, AU - Ghandil,Pegah, AU - Jafari,Afshar, AU - Saki Malehi,Amal, Y1 - 2019/04/26/ PY - 2018/08/14/received PY - 2018/10/26/revised PY - 2019/02/07/accepted PY - 2019/5/31/entrez PY - 2019/5/31/pubmed PY - 2019/5/31/medline KW - Exercise-induced adaptation KW - heat shock proteins KW - natural antioxidants SP - 37 EP - 37 JF - Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences JO - J Res Med Sci VL - 24 N2 - Background: The aim of the present study was to evaluate the effect of natural antioxidant formula (blend of herbs: ginger root, cinnamon bark and raw almond fruit powder, rosemary leaf powder, and honey) on oxidative status, antioxidant enzyme activity, and relative heat shock protein (HSP-70) expression in recreational female athletes. Materials and Methods: Eighteen female participants trained for 4 weeks and randomly received either antioxidant formula (FormEX) (n = 8) or placebo (PlcEX) (n = 10) in a randomized controlled trial. Blood samples were obtained 1-h before, 1 h and 24 h postexercise to measure malondialdehyde (MDA), total antioxidant capacity (TAC), superoxide dismutase (SOD), glutathione peroxidases (GPx), and HSP70 mRNA expression. Data analysis was performed using 2 (treatment = grouping factor) ×6 (time = within-factor) repeated measurements analysis of variance or generalized estimating equations (GEE) test. We used the independent t-test to evaluate any significant differences for real-time polymerase chain reaction data. Results: Antioxidant formula increased the relative HSP-70 mRNA expression more than Plc-EX group in all time points (P = 0.001). The time main effect was significant with regard to TAC and SOD concentrations (P = 0.001 and 0.002, respectively). However, there were no statistically significant differences between groups for TAC, SOD, and MDA (P = 0.25, 0.06, and 0.38, respectively). Neither the time main effect for MDA nor time and intervention interaction was not statistically significant for MDA, TAC, and SOD (P = 0.19, 0.13, and 0.10, respectively). GEE results for GPx showed that there were no significant differences between the groups (P = 0.11). Conclusion: The results presented herein revealed that natural antioxidant rich formula had variable effects on oxidative status. However, in contrast to many antioxidant supplements, this formulation increases the HSP-70 mRNA expression which might improve the antioxidant ability of cells in the long-term period and exercise-induced adaptation. SN - 1735-1995 UR - https://www.unboundmedicine.com/medline/citation/31143238/Effect_of_herbal_antioxidant-rich_formula_on_improvement_of_antioxidant_defense_system_and_heat_shock_protein-70_expression_in_recreational_female_athletes:_A_randomized_controlled_trial L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/31143238/ DB - PRIME DP - Unbound Medicine ER -