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A histological, immunohistochemical and biochemical study of the effects of pomegranate peel extracts on gibberellic acid induced oxidative stress in adult rat testes.

Abstract

Gibberellins are commonly used plant growth regulators that exhibit deleterious effects on various animal tissues. We investigated the histological, immunohistochemical and biochemical effects of gibberellic acid (GA3) on rat testes as well as the possible protective role of pomegranate peel extract (PPE). We used 28 adult male rats divided into control, PPE treated, GS3 treated and GA3 + PPE treated groups. Testis specimens were analyzed for superoxide dismutase (SOD) and catalase (CAT) activity, and examined histologically. We also investigated the androgen receptor using immunohistochemistry. The GA3 treated group exhibited significantly decreased SOD and CAT levels and area percent of androgen receptor. Seminiferous tubules (ST) were widely separated and the germinal epithelium was separated from the basement membrane in some tubules. Areas of vacuolation, degenerated germ cells with pyknotic nuclei and large multinucleated cells were observed. Ultrastructurally, primary spermatocytes exhibited vacuolated cytoplasm, degenerated mitochondria and hyperchromatic nuclei. Degenerated early spermatids with a ruptured or hyperchromatic nucleus were found. Spermatozoa exhibited abnormalities of the head and tail. The cytoplasm of Sertoli and Leydig cells exhibited dilated smooth endoplasmic reticulum. A significant improvement of the biochemical, histological and immunohistochemical alterations was observed in the GA3 + PPE treated group compared to the GA3 treated group.

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  • Authors+Show Affiliations

    ,

    a Histology and Cell Biology Department, Faculty of Medicine , Mansoura University , Al Mansurah , Egypt.

    ,

    a Histology and Cell Biology Department, Faculty of Medicine , Mansoura University , Al Mansurah , Egypt.

    a Histology and Cell Biology Department, Faculty of Medicine , Mansoura University , Al Mansurah , Egypt.

    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31144534

    Citation

    Khalaf, H A., et al. "A Histological, Immunohistochemical and Biochemical Study of the Effects of Pomegranate Peel Extracts On Gibberellic Acid Induced Oxidative Stress in Adult Rat Testes." Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission, 2019, pp. 1-14.
    Khalaf HA, Arafat EA, Ghoneim FM. A histological, immunohistochemical and biochemical study of the effects of pomegranate peel extracts on gibberellic acid induced oxidative stress in adult rat testes. Biotech Histochem. 2019.
    Khalaf, H. A., Arafat, E. A., & Ghoneim, F. M. (2019). A histological, immunohistochemical and biochemical study of the effects of pomegranate peel extracts on gibberellic acid induced oxidative stress in adult rat testes. Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission, pp. 1-14. doi:10.1080/10520295.2019.1602884.
    Khalaf HA, Arafat EA, Ghoneim FM. A Histological, Immunohistochemical and Biochemical Study of the Effects of Pomegranate Peel Extracts On Gibberellic Acid Induced Oxidative Stress in Adult Rat Testes. Biotech Histochem. 2019 May 30;1-14. PubMed PMID: 31144534.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - A histological, immunohistochemical and biochemical study of the effects of pomegranate peel extracts on gibberellic acid induced oxidative stress in adult rat testes. AU - Khalaf,H A, AU - Arafat,E A, AU - Ghoneim,F M, Y1 - 2019/05/30/ PY - 2019/5/31/entrez KW - Electron microscopy KW - gibberellic acid KW - oxidative stress KW - pomegranate KW - rats KW - testis SP - 1 EP - 14 JF - Biotechnic & histochemistry : official publication of the Biological Stain Commission JO - Biotech Histochem N2 - Gibberellins are commonly used plant growth regulators that exhibit deleterious effects on various animal tissues. We investigated the histological, immunohistochemical and biochemical effects of gibberellic acid (GA3) on rat testes as well as the possible protective role of pomegranate peel extract (PPE). We used 28 adult male rats divided into control, PPE treated, GS3 treated and GA3 + PPE treated groups. Testis specimens were analyzed for superoxide dismutase (SOD) and catalase (CAT) activity, and examined histologically. We also investigated the androgen receptor using immunohistochemistry. The GA3 treated group exhibited significantly decreased SOD and CAT levels and area percent of androgen receptor. Seminiferous tubules (ST) were widely separated and the germinal epithelium was separated from the basement membrane in some tubules. Areas of vacuolation, degenerated germ cells with pyknotic nuclei and large multinucleated cells were observed. Ultrastructurally, primary spermatocytes exhibited vacuolated cytoplasm, degenerated mitochondria and hyperchromatic nuclei. Degenerated early spermatids with a ruptured or hyperchromatic nucleus were found. Spermatozoa exhibited abnormalities of the head and tail. The cytoplasm of Sertoli and Leydig cells exhibited dilated smooth endoplasmic reticulum. A significant improvement of the biochemical, histological and immunohistochemical alterations was observed in the GA3 + PPE treated group compared to the GA3 treated group. SN - 1473-7760 UR - https://www.unboundmedicine.com/medline/citation/31144534/A_histological,_immunohistochemical_and_biochemical_study_of_the_effects_of_pomegranate_peel_extracts_on_gibberellic_acid_induced_oxidative_stress_in_adult_rat_testes L2 - http://www.tandfonline.com/doi/full/10.1080/10520295.2019.1602884 DB - PRIME DP - Unbound Medicine ER -