Tags

Type your tag names separated by a space and hit enter

Dynamics of Plasmodium falciparum genetic diversity among asymptomatic and symptomatic children in three epidemiological areas in Cote d'Ivoire.
Pathog Glob Health. 2019 05; 113(3):133-142.PG

Abstract

Asymptomatic carriers of Plasmodium are considered a reservoir of the parasite in humans. Therefore, in order to be effective, new malaria elimination strategies must take these targets into account. The aim of this study was to analyse genetic diversity of Plasmodium falciparum among schoolchildren in three epidemiological areas in Côte d'Ivoire. This was a cross-sectional study carried out from May 2015 to April 2016 in a primary school in rural and urban areas of San Pedro, Grand-Bassam and Abengourou, during the rainy season and the dry season. A total of 282 Plasmodium falciparum isolates were genotyped using Nested PCR of Pfmsp1 and Pfmsp2 genes. The overall frequency of K1, Mad20 and RO33 alleles was 81.6%, 53.4% and 57% for Pfmsp1 respectively. For Pfmsp2, this frequency was 84.3% and 72.2% for 3D7 and FC27. K1, Mad20 and FC27 Frequencies were significantly higher in Abengourou compared to other sites. Overall, the frequency of MIs was significantly higher in Abengourou for Pfmsp1 and Pfmsp2. However, Mad20 and RO33 alleles were significantly higher in the rainy season. No significant difference was observed between Pfmsp2 alleles in both seasons. Frequency of the 3D7 allele was significantly higher in symptomatic patients. MIs and COI increased with parasitemia for Pfmsp1and Pfmsp2. The data can be added to that available for monitoring and control of P. falciparum malaria. Further studies combining the entomological inoculation rate and the genetic diversity of P. falciparum will allow us to shed light on our understanding of the epidemiology of this parasite.

Authors+Show Affiliations

a Centre de Recherche et de Lutte contre le Paludisme , Institut National de Santé Publique , Abidjan , Côte d'Ivoire. b Laboratoire de Génétique , Université Félix Houphouët Boigny , Abidjan , Côte d'Ivoire.a Centre de Recherche et de Lutte contre le Paludisme , Institut National de Santé Publique , Abidjan , Côte d'Ivoire. c Département de Parasitologie-Mycologie , UFR Sciences Pharmaceutiques et Biologique , Abidjan , Côte d'Ivoire.a Centre de Recherche et de Lutte contre le Paludisme , Institut National de Santé Publique , Abidjan , Côte d'Ivoire. c Département de Parasitologie-Mycologie , UFR Sciences Pharmaceutiques et Biologique , Abidjan , Côte d'Ivoire.a Centre de Recherche et de Lutte contre le Paludisme , Institut National de Santé Publique , Abidjan , Côte d'Ivoire. b Laboratoire de Génétique , Université Félix Houphouët Boigny , Abidjan , Côte d'Ivoire.c Département de Parasitologie-Mycologie , UFR Sciences Pharmaceutiques et Biologique , Abidjan , Côte d'Ivoire.b Laboratoire de Génétique , Université Félix Houphouët Boigny , Abidjan , Côte d'Ivoire.a Centre de Recherche et de Lutte contre le Paludisme , Institut National de Santé Publique , Abidjan , Côte d'Ivoire. c Département de Parasitologie-Mycologie , UFR Sciences Pharmaceutiques et Biologique , Abidjan , Côte d'Ivoire.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31144611

Citation

Gnagne, Akpa Paterne, et al. "Dynamics of Plasmodium Falciparum Genetic Diversity Among Asymptomatic and Symptomatic Children in Three Epidemiological Areas in Cote D'Ivoire." Pathogens and Global Health, vol. 113, no. 3, 2019, pp. 133-142.
Gnagne AP, Konate A, Bedia-Tanoh AV, et al. Dynamics of Plasmodium falciparum genetic diversity among asymptomatic and symptomatic children in three epidemiological areas in Cote d'Ivoire. Pathog Glob Health. 2019;113(3):133-142.
Gnagne, A. P., Konate, A., Bedia-Tanoh, A. V., Amiah-Droh, M., Menan, H. I. E., N'Guetta, A. S., & Yavo, W. (2019). Dynamics of Plasmodium falciparum genetic diversity among asymptomatic and symptomatic children in three epidemiological areas in Cote d'Ivoire. Pathogens and Global Health, 113(3), 133-142. https://doi.org/10.1080/20477724.2019.1624076
Gnagne AP, et al. Dynamics of Plasmodium Falciparum Genetic Diversity Among Asymptomatic and Symptomatic Children in Three Epidemiological Areas in Cote D'Ivoire. Pathog Glob Health. 2019;113(3):133-142. PubMed PMID: 31144611.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dynamics of Plasmodium falciparum genetic diversity among asymptomatic and symptomatic children in three epidemiological areas in Cote d'Ivoire. AU - Gnagne,Akpa Paterne, AU - Konate,Abibatou, AU - Bedia-Tanoh,Akoua Valérie, AU - Amiah-Droh,Mireille, AU - Menan,Hervé Ignace Eby, AU - N'Guetta,Assanvo Simon-Pierre, AU - Yavo,William, Y1 - 2019/05/30/ PY - 2019/5/31/pubmed PY - 2020/1/10/medline PY - 2019/5/31/entrez KW - KW - Côte d’Ivoire KW - asymptomatic carrier KW - genetic diversity SP - 133 EP - 142 JF - Pathogens and global health JO - Pathog Glob Health VL - 113 IS - 3 N2 - Asymptomatic carriers of Plasmodium are considered a reservoir of the parasite in humans. Therefore, in order to be effective, new malaria elimination strategies must take these targets into account. The aim of this study was to analyse genetic diversity of Plasmodium falciparum among schoolchildren in three epidemiological areas in Côte d'Ivoire. This was a cross-sectional study carried out from May 2015 to April 2016 in a primary school in rural and urban areas of San Pedro, Grand-Bassam and Abengourou, during the rainy season and the dry season. A total of 282 Plasmodium falciparum isolates were genotyped using Nested PCR of Pfmsp1 and Pfmsp2 genes. The overall frequency of K1, Mad20 and RO33 alleles was 81.6%, 53.4% and 57% for Pfmsp1 respectively. For Pfmsp2, this frequency was 84.3% and 72.2% for 3D7 and FC27. K1, Mad20 and FC27 Frequencies were significantly higher in Abengourou compared to other sites. Overall, the frequency of MIs was significantly higher in Abengourou for Pfmsp1 and Pfmsp2. However, Mad20 and RO33 alleles were significantly higher in the rainy season. No significant difference was observed between Pfmsp2 alleles in both seasons. Frequency of the 3D7 allele was significantly higher in symptomatic patients. MIs and COI increased with parasitemia for Pfmsp1and Pfmsp2. The data can be added to that available for monitoring and control of P. falciparum malaria. Further studies combining the entomological inoculation rate and the genetic diversity of P. falciparum will allow us to shed light on our understanding of the epidemiology of this parasite. SN - 2047-7732 UR - https://www.unboundmedicine.com/medline/citation/31144611/Dynamics_of_Plasmodium_falciparum_genetic_diversity_among_asymptomatic_and_symptomatic_children_in_three_epidemiological_areas_in_Cote_d'Ivoire_ L2 - https://www.tandfonline.com/doi/full/10.1080/20477724.2019.1624076 DB - PRIME DP - Unbound Medicine ER -