Tags

Type your tag names separated by a space and hit enter

Broiler chicken myofiber morphometrics and myogenic stem cell population heterogeneity1,2.
Poult Sci. 2019 Sep 01; 98(9):4123-4130.PS

Abstract

Myogenic stem cells (MSC), also known as satellite cells, play a key role in mediating post-hatch skeletal muscle growth in broiler chickens. Information regarding the heterogeneity of MSC populations and myofiber size distributions in different muscles of modern broiler strains of both sexes is scarce. Thus, myofiber cross-sectional area (CSA) and myogenic regulatory factor expression by MSC populations in 2 functionally different muscles (Biceps femoris, BF and Pectoralis major, PM) from both sexes of broilers from 2 strains, Ross 708 x Ross 708 (ROSS; Aviagen Group, Huntsville, AL) and Red Ranger (RR; S&G Poultry, Clanton, AL) was evaluated. Female and male broilers from both strains (n = 80 birds per strain) were reared for 43 d on a common corn and soybean meal-based diet. At 43 d of age, 8 birds per sex per strain (n = 32 total) were euthanized and PM and BF muscle samples from each bird were analyzed using cryohistology, immunofluorescence (IF) staining, and fluorescence microscopy. Cryosections from each muscle from each bird were IF stained to detect Myf-5, MyoD, and Pax7-expressing MSC. The CSA of every fiber in each digital image was measured and MSC populations (Myf-5+, MyoD+, Pax7+, Myf-5+: MyoD+, Myf-5+: Pax7+, MyoD+: Pax7+, and Myf-5+: MyoD+: Pax7+) were enumerated. Myofiber CSA was similar among the 2 strains and 2 sexes (P > 0.05). As expected, mean myofiber CSA was greater in the predominantly fast-twitch PM than the predominantly slow-twitch BF muscle (P = 0.006). At 43 d of age, ROSS broilers had a 32% larger population of Myf-5+: MyoD+: Pax7+ MSC compared with RR (P = 0.04). The heterogeneity of MSC was similar among the sexes. While the total MSC population was larger in the BF muscle compared with the PM (P < 0.05), the Myf-5+ and Pax7+ MSC densities were similar among muscles (P > 0.05). The major differences in myofiber morphometrics and MSC heterogeneity were not among the different broiler strains or sexes but were instead, between the 2 functionally different muscles.

Authors+Show Affiliations

Department of Poultry Science, Auburn University, Auburn, AL 38649.Department of Poultry Science, Auburn University, Auburn, AL 38649.Department of Poultry Science, Auburn University, Auburn, AL 38649.Department of Poultry Science, Auburn University, Auburn, AL 38649.Department of Poultry Science, Auburn University, Auburn, AL 38649.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31144722

Citation

Tejeda, O J., et al. "Broiler Chicken Myofiber Morphometrics and Myogenic Stem Cell Population Heterogeneity1,2." Poultry Science, vol. 98, no. 9, 2019, pp. 4123-4130.
Tejeda OJ, Calderon AJ, Arana JA, et al. Broiler chicken myofiber morphometrics and myogenic stem cell population heterogeneity1,2. Poult Sci. 2019;98(9):4123-4130.
Tejeda, O. J., Calderon, A. J., Arana, J. A., Meloche, K. J., & Starkey, J. D. (2019). Broiler chicken myofiber morphometrics and myogenic stem cell population heterogeneity1,2. Poultry Science, 98(9), 4123-4130. https://doi.org/10.3382/ps/pez287
Tejeda OJ, et al. Broiler Chicken Myofiber Morphometrics and Myogenic Stem Cell Population Heterogeneity1,2. Poult Sci. 2019 Sep 1;98(9):4123-4130. PubMed PMID: 31144722.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Broiler chicken myofiber morphometrics and myogenic stem cell population heterogeneity1,2. AU - Tejeda,O J, AU - Calderon,A J, AU - Arana,J A, AU - Meloche,K J, AU - Starkey,J D, PY - 2019/02/02/received PY - 2019/05/07/accepted PY - 2019/5/31/pubmed PY - 2019/11/27/medline PY - 2019/5/31/entrez KW - broiler KW - myofiber KW - myogenic stem cell KW - satellite cell KW - skeletal muscle SP - 4123 EP - 4130 JF - Poultry science JO - Poult Sci VL - 98 IS - 9 N2 - Myogenic stem cells (MSC), also known as satellite cells, play a key role in mediating post-hatch skeletal muscle growth in broiler chickens. Information regarding the heterogeneity of MSC populations and myofiber size distributions in different muscles of modern broiler strains of both sexes is scarce. Thus, myofiber cross-sectional area (CSA) and myogenic regulatory factor expression by MSC populations in 2 functionally different muscles (Biceps femoris, BF and Pectoralis major, PM) from both sexes of broilers from 2 strains, Ross 708 x Ross 708 (ROSS; Aviagen Group, Huntsville, AL) and Red Ranger (RR; S&G Poultry, Clanton, AL) was evaluated. Female and male broilers from both strains (n = 80 birds per strain) were reared for 43 d on a common corn and soybean meal-based diet. At 43 d of age, 8 birds per sex per strain (n = 32 total) were euthanized and PM and BF muscle samples from each bird were analyzed using cryohistology, immunofluorescence (IF) staining, and fluorescence microscopy. Cryosections from each muscle from each bird were IF stained to detect Myf-5, MyoD, and Pax7-expressing MSC. The CSA of every fiber in each digital image was measured and MSC populations (Myf-5+, MyoD+, Pax7+, Myf-5+: MyoD+, Myf-5+: Pax7+, MyoD+: Pax7+, and Myf-5+: MyoD+: Pax7+) were enumerated. Myofiber CSA was similar among the 2 strains and 2 sexes (P > 0.05). As expected, mean myofiber CSA was greater in the predominantly fast-twitch PM than the predominantly slow-twitch BF muscle (P = 0.006). At 43 d of age, ROSS broilers had a 32% larger population of Myf-5+: MyoD+: Pax7+ MSC compared with RR (P = 0.04). The heterogeneity of MSC was similar among the sexes. While the total MSC population was larger in the BF muscle compared with the PM (P < 0.05), the Myf-5+ and Pax7+ MSC densities were similar among muscles (P > 0.05). The major differences in myofiber morphometrics and MSC heterogeneity were not among the different broiler strains or sexes but were instead, between the 2 functionally different muscles. SN - 1525-3171 UR - https://www.unboundmedicine.com/medline/citation/31144722/Broiler_chicken_myofiber_morphometrics_and_myogenic_stem_cell_population_heterogeneity12_ DB - PRIME DP - Unbound Medicine ER -