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The sperm associated antigen 6 interactome and its role in spermatogenesis.

Abstract

Mammalian SPAG6, the orthologue of Chlamydomonas reinhardtii PF16, is a component of the central apparatus of the "9+2" axoneme that controls ciliary/flagellar motility, including sperm motility. Recent studies revealed that SPAG6 has functions beyond its role in the central apparatus. Hence, we re-examined the role of SPAG6 in male fertility. In wild-type mice, SPAG6 was present in cytoplasmic vesicles in spermatocytes, the acrosome of round and elongating spermatids, and the manchette of elongating spermatids. Spag6-deficient testes showed abnormal spermatogenesis, with abnormalities in male germ cell morphology consistent with the multi-compartment pattern of SPAG6 localization. The armadillo repeat domain of mouse SPAG6 was used as a bait in a yeast two-hybrid screen, and several proteins with diverse functions appeared multiple times, including Snapin, SPINK2, and COPS5. Snapin has a similar localization to SPAG6 in male germ cells, and SPINK2, a key protein in acrosome biogenesis, was dramatically reduced in Spag6-deficient mice which have defective acrosomes. SPAG16L, another SPAG6 binding partner, lost its localization to the manchette in Spag6-deficient mice. Our findings demonstrate that SPAG6 is a multi-functional protein that not only regulates sperm motility, but also plays roles in spermatogenesis in multiple cellular compartments involving multiple protein partners.

Authors+Show Affiliations

Y Liu, School of Public Health, Wuhan University of Science and Technology, Wuhan, 430081, China.L Zhang, School of Public Health, Wuhan University of Science and Technology, Wuhan, China.W Li, Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, United States.Q Huang, School of Public Health, Wuhan University of Science and Technology, Wuhan, China.S Yuan, School of Public Health, Wuhan University of Science and Technology, Wuhan, China.Y Li, School of Public Health, Wuhan University of Science and Technology, Wuhan, China.J Liu, School of Public Health, Wuhan University of Science and Technology, Wuhan, China.S Zhang, School of Public Health, Wuhan University of Science and Technology, Wuhan, China.G Pin, School of Public Health, Wuhan University of Science and Technology, Wuhan, China.S Song, School of Public Health, Wuhan University of Science and Technology, Wuhan, China.P Ray, GETI, Institute for advanced Biosciences, LA TRONCHE, France.C Arnoult, Genetic Epigenetic and Therapies of Infertility, Institute for advanced Biosciences, Grenoble, France.C Cho, School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Korea (the Republic of).B Garcia-Reyes, Department of General and Visceral Surgery, Ulm University, Ulm, Germany.U Knippschild, Department of General and Visceral Surgery, Ulm University, Ulm, Germany.J Strauss III, Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, United States.Z Zhang, Department of Physiology, Wayne State University, Detroit, United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31146259

Citation

Liu, Yunhao, et al. "The Sperm Associated Antigen 6 Interactome and Its Role in Spermatogenesis." Reproduction (Cambridge, England), 2019.
Liu Y, Zhang L, Li W, et al. The sperm associated antigen 6 interactome and its role in spermatogenesis. Reproduction. 2019.
Liu, Y., Zhang, L., Li, W., Huang, Q., Yuan, S., Li, Y., ... Zhang, Z. (2019). The sperm associated antigen 6 interactome and its role in spermatogenesis. Reproduction (Cambridge, England), doi:10.1530/REP-18-0522.
Liu Y, et al. The Sperm Associated Antigen 6 Interactome and Its Role in Spermatogenesis. Reproduction. 2019 May 1; PubMed PMID: 31146259.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The sperm associated antigen 6 interactome and its role in spermatogenesis. AU - Liu,Yunhao, AU - Zhang,Ling, AU - Li,Wei, AU - Huang,Qian, AU - Yuan,Shuo, AU - Li,Yuhong, AU - Liu,Junpin, AU - Zhang,Shiyang, AU - Pin,Guanglun, AU - Song,Shizhen, AU - Ray,Pierre F, AU - Arnoult,Christophe, AU - Cho,Chunghee, AU - Garcia-Reyes,Balbina, AU - Knippschild,Uwe, AU - Strauss Iii,Jerome F, AU - Zhang,Zhibing, Y1 - 2019/05/01/ PY - 2018/10/04/received PY - 2019/05/30/accepted PY - 2019/5/31/pubmed PY - 2019/5/31/medline PY - 2019/5/31/entrez JF - Reproduction (Cambridge, England) JO - Reproduction N2 - Mammalian SPAG6, the orthologue of Chlamydomonas reinhardtii PF16, is a component of the central apparatus of the "9+2" axoneme that controls ciliary/flagellar motility, including sperm motility. Recent studies revealed that SPAG6 has functions beyond its role in the central apparatus. Hence, we re-examined the role of SPAG6 in male fertility. In wild-type mice, SPAG6 was present in cytoplasmic vesicles in spermatocytes, the acrosome of round and elongating spermatids, and the manchette of elongating spermatids. Spag6-deficient testes showed abnormal spermatogenesis, with abnormalities in male germ cell morphology consistent with the multi-compartment pattern of SPAG6 localization. The armadillo repeat domain of mouse SPAG6 was used as a bait in a yeast two-hybrid screen, and several proteins with diverse functions appeared multiple times, including Snapin, SPINK2, and COPS5. Snapin has a similar localization to SPAG6 in male germ cells, and SPINK2, a key protein in acrosome biogenesis, was dramatically reduced in Spag6-deficient mice which have defective acrosomes. SPAG16L, another SPAG6 binding partner, lost its localization to the manchette in Spag6-deficient mice. Our findings demonstrate that SPAG6 is a multi-functional protein that not only regulates sperm motility, but also plays roles in spermatogenesis in multiple cellular compartments involving multiple protein partners. SN - 1741-7899 UR - https://www.unboundmedicine.com/medline/citation/31146259/The_sperm_associated_antigen_6_interactome_and_its_role_in_spermatogenesis L2 - https://rep.bioscientifica.com/doi/10.1530/REP-18-0522 DB - PRIME DP - Unbound Medicine ER -