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The sperm associated antigen 6 interactome and its role in spermatogenesis.

Abstract

Mammalian SPAG6, the orthologue of Chlamydomonas reinhardtii PF16, is a component of the central apparatus of the "9+2" axoneme that controls ciliary/flagellar motility, including sperm motility. Recent studies revealed that SPAG6 has functions beyond its role in the central apparatus. Hence, we re-examined the role of SPAG6 in male fertility. In wild-type mice, SPAG6 was present in cytoplasmic vesicles in spermatocytes, the acrosome of round and elongating spermatids, and the manchette of elongating spermatids. Spag6-deficient testes showed abnormal spermatogenesis, with abnormalities in male germ cell morphology consistent with the multi-compartment pattern of SPAG6 localization. The armadillo repeat domain of mouse SPAG6 was used as a bait in a yeast two-hybrid screen, and several proteins with diverse functions appeared multiple times, including Snapin, SPINK2, and COPS5. Snapin has a similar localization to SPAG6 in male germ cells, and SPINK2, a key protein in acrosome biogenesis, was dramatically reduced in Spag6-deficient mice which have defective acrosomes. SPAG16L, another SPAG6 binding partner, lost its localization to the manchette in Spag6-deficient mice. Our findings demonstrate that SPAG6 is a multi-functional protein that not only regulates sperm motility, but also plays roles in spermatogenesis in multiple cellular compartments involving multiple protein partners.

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  • Authors+Show Affiliations

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    Y Liu, School of Public Health, Wuhan University of Science and Technology, Wuhan, 430081, China.

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    L Zhang, School of Public Health, Wuhan University of Science and Technology, Wuhan, China.

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    W Li, Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, United States.

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    Q Huang, School of Public Health, Wuhan University of Science and Technology, Wuhan, China.

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    S Yuan, School of Public Health, Wuhan University of Science and Technology, Wuhan, China.

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    Y Li, School of Public Health, Wuhan University of Science and Technology, Wuhan, China.

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    J Liu, School of Public Health, Wuhan University of Science and Technology, Wuhan, China.

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    S Zhang, School of Public Health, Wuhan University of Science and Technology, Wuhan, China.

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    G Pin, School of Public Health, Wuhan University of Science and Technology, Wuhan, China.

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    S Song, School of Public Health, Wuhan University of Science and Technology, Wuhan, China.

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    P Ray, GETI, Institute for advanced Biosciences, LA TRONCHE, France.

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    C Arnoult, Genetic Epigenetic and Therapies of Infertility, Institute for advanced Biosciences, Grenoble, France.

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    C Cho, School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Korea (the Republic of).

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    B Garcia-Reyes, Department of General and Visceral Surgery, Ulm University, Ulm, Germany.

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    U Knippschild, Department of General and Visceral Surgery, Ulm University, Ulm, Germany.

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    J Strauss III, Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, United States.

    Z Zhang, Department of Physiology, Wayne State University, Detroit, United States.

    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31146259

    Citation

    Liu, Yunhao, et al. "The Sperm Associated Antigen 6 Interactome and Its Role in Spermatogenesis." Reproduction (Cambridge, England), 2019.
    Liu Y, Zhang L, Li W, et al. The sperm associated antigen 6 interactome and its role in spermatogenesis. Reproduction. 2019.
    Liu, Y., Zhang, L., Li, W., Huang, Q., Yuan, S., Li, Y., ... Zhang, Z. (2019). The sperm associated antigen 6 interactome and its role in spermatogenesis. Reproduction (Cambridge, England), doi:10.1530/REP-18-0522.
    Liu Y, et al. The Sperm Associated Antigen 6 Interactome and Its Role in Spermatogenesis. Reproduction. 2019 May 1; PubMed PMID: 31146259.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - The sperm associated antigen 6 interactome and its role in spermatogenesis. AU - Liu,Yunhao, AU - Zhang,Ling, AU - Li,Wei, AU - Huang,Qian, AU - Yuan,Shuo, AU - Li,Yuhong, AU - Liu,Junpin, AU - Zhang,Shiyang, AU - Pin,Guanglun, AU - Song,Shizhen, AU - Ray,Pierre F, AU - Arnoult,Christophe, AU - Cho,Chunghee, AU - Garcia-Reyes,Balbina, AU - Knippschild,Uwe, AU - Strauss Iii,Jerome F, AU - Zhang,Zhibing, Y1 - 2019/05/01/ PY - 2018/10/04/received PY - 2019/05/30/accepted PY - 2019/5/31/pubmed PY - 2019/5/31/medline PY - 2019/5/31/entrez JF - Reproduction (Cambridge, England) JO - Reproduction N2 - Mammalian SPAG6, the orthologue of Chlamydomonas reinhardtii PF16, is a component of the central apparatus of the "9+2" axoneme that controls ciliary/flagellar motility, including sperm motility. Recent studies revealed that SPAG6 has functions beyond its role in the central apparatus. Hence, we re-examined the role of SPAG6 in male fertility. In wild-type mice, SPAG6 was present in cytoplasmic vesicles in spermatocytes, the acrosome of round and elongating spermatids, and the manchette of elongating spermatids. Spag6-deficient testes showed abnormal spermatogenesis, with abnormalities in male germ cell morphology consistent with the multi-compartment pattern of SPAG6 localization. The armadillo repeat domain of mouse SPAG6 was used as a bait in a yeast two-hybrid screen, and several proteins with diverse functions appeared multiple times, including Snapin, SPINK2, and COPS5. Snapin has a similar localization to SPAG6 in male germ cells, and SPINK2, a key protein in acrosome biogenesis, was dramatically reduced in Spag6-deficient mice which have defective acrosomes. SPAG16L, another SPAG6 binding partner, lost its localization to the manchette in Spag6-deficient mice. Our findings demonstrate that SPAG6 is a multi-functional protein that not only regulates sperm motility, but also plays roles in spermatogenesis in multiple cellular compartments involving multiple protein partners. SN - 1741-7899 UR - https://www.unboundmedicine.com/medline/citation/31146259/The_sperm_associated_antigen_6_interactome_and_its_role_in_spermatogenesis L2 - https://rep.bioscientifica.com/doi/10.1530/REP-18-0522 DB - PRIME DP - Unbound Medicine ER -