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In Silico Genome-Wide Analysis of Respiratory Burst Oxidase Homolog (RBOH) Family Genes in Five Fruit-Producing Trees, and Potential Functional Analysis on Lignification of Stone Cells in Chinese White Pear.
Cells. 2019 05 29; 8(6)C

Abstract

: The accumulation of lignin in fruit has a significant negative impact on the quality of fruit-producing trees, and in particular the lignin formation stimulates the development of stone cells in pear fruit. Reactive oxygen species (ROS) are essential for lignin polymerization. However, knowledge of the RBOH family, a key enzyme in ROS metabolism, remains unknown in most fruit trees. In this study, a total of 40 RBOHs were identified from five fruit-producing trees (Pyrus bretschneideri, Prunus persica, Citrus sinensis, Vitis vinifera, and Prunus mume), and 10 of these sequences came from Pyrus bretschneideri. Multiple sequence alignments revealed that all 10 PbRBOHs contained the NADPH_Ox domain and the six alpha-helical transmembrane domains (TM-I to TM-VI). Chromosome localization and interspecies phylogenetic tree analysis showed that 10 PbRBOHs irregularly distributed on 8 chromosomes and 3 PbRBOHs (PbRBOHA, PbRBOHB, and PbRBOHD) are closely related to known lignification-related RBOHs. Furthermore, hormone response pattern analysis showed that the transcription of PbRBOHs is regulated by SA, ABA and MeJA. Reverse transcription-quantitative real-time polymerase chain reaction (qRT-PCR) and transcriptome sequencing analysis showed that PbRBOHA, PbRBOHB, and PbRBOHD accumulated high transcript abundance in pear fruit, and the transcriptional trends of PbRBOHA and PbRBOHD was consistent with the change of stone cell content during fruit development. In addition, subcellular localization revealed that PbRBOHA and PbRBOHD are distributed on the plasma membrane. Combining the changes of apoplastic superoxide (O2.-) content and spatio-temporal expression analysis, these results indicate that PbRBOHA and PbRBOHD, which are candidate genes, may play an important role in ROS metabolism during the lignification of pear stone cells. This study not only provided insight into the molecular characteristics of the RBOH family in fruit-producing trees, but also lays the foundation for studying the role of ROS in plant lignification.

Authors+Show Affiliations

School of Life Science, Anhui Agricultural University, No. 130, Changjiang West Road, Hefei 230036, China. chengxi90@ahau.edu.cn.School of Life Science, Anhui Agricultural University, No. 130, Changjiang West Road, Hefei 230036, China. xh16720349@ahau.edu.cn.School of Life Science, Anhui Agricultural University, No. 130, Changjiang West Road, Hefei 230036, China. alamsher@ahau.edu.cn.School of Life Science, Anhui Agricultural University, No. 130, Changjiang West Road, Hefei 230036, China. skywh@ahau.edu.cn.School of Life Science, Anhui Agricultural University, No. 130, Changjiang West Road, Hefei 230036, China. abdullahpadana@ahau.edu.cn.School of Life Science, Anhui Agricultural University, No. 130, Changjiang West Road, Hefei 230036, China. 15003011@ahau.edu.cn.Horticultural Institute, Anhui Academy of Agricultural Sciences, Hefei, Anhui 230031, China. 16112378@ahau.edu.cn.School of Life Science, Anhui Agricultural University, No. 130, Changjiang West Road, Hefei 230036, China. jts2015@ahau.edu.cn.School of Life Science, Anhui Agricultural University, No. 130, Changjiang West Road, Hefei 230036, China. qingjin@ahau.edu.cn.School of Life Science, Anhui Agricultural University, No. 130, Changjiang West Road, Hefei 230036, China. ypcaiah@163.com.School of Life Science, Anhui Agricultural University, No. 130, Changjiang West Road, Hefei 230036, China. yjsc01@ahau.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31146469

Citation

Cheng, Xi, et al. "In Silico Genome-Wide Analysis of Respiratory Burst Oxidase Homolog (RBOH) Family Genes in Five Fruit-Producing Trees, and Potential Functional Analysis On Lignification of Stone Cells in Chinese White Pear." Cells, vol. 8, no. 6, 2019.
Cheng X, Li G, Manzoor MA, et al. In Silico Genome-Wide Analysis of Respiratory Burst Oxidase Homolog (RBOH) Family Genes in Five Fruit-Producing Trees, and Potential Functional Analysis on Lignification of Stone Cells in Chinese White Pear. Cells. 2019;8(6).
Cheng, X., Li, G., Manzoor, M. A., Wang, H., Abdullah, M., Su, X., Zhang, J., Jiang, T., Jin, Q., Cai, Y., & Lin, Y. (2019). In Silico Genome-Wide Analysis of Respiratory Burst Oxidase Homolog (RBOH) Family Genes in Five Fruit-Producing Trees, and Potential Functional Analysis on Lignification of Stone Cells in Chinese White Pear. Cells, 8(6). https://doi.org/10.3390/cells8060520
Cheng X, et al. In Silico Genome-Wide Analysis of Respiratory Burst Oxidase Homolog (RBOH) Family Genes in Five Fruit-Producing Trees, and Potential Functional Analysis On Lignification of Stone Cells in Chinese White Pear. Cells. 2019 05 29;8(6) PubMed PMID: 31146469.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In Silico Genome-Wide Analysis of Respiratory Burst Oxidase Homolog (RBOH) Family Genes in Five Fruit-Producing Trees, and Potential Functional Analysis on Lignification of Stone Cells in Chinese White Pear. AU - Cheng,Xi, AU - Li,Guohui, AU - Manzoor,Muhammad Aamir, AU - Wang,Han, AU - Abdullah,Muhammad, AU - Su,Xueqiang, AU - Zhang,Jingyun, AU - Jiang,Taoshan, AU - Jin,Qing, AU - Cai,Yongping, AU - Lin,Yi, Y1 - 2019/05/29/ PY - 2019/04/08/received PY - 2019/05/22/revised PY - 2019/05/28/accepted PY - 2019/6/1/entrez PY - 2019/5/31/pubmed PY - 2019/5/31/medline KW - ROS KW - lignification KW - pear KW - phylogenetic and expression analysis KW - respiratory burst oxidase homolog family KW - subcellular localization JF - Cells JO - Cells VL - 8 IS - 6 N2 - : The accumulation of lignin in fruit has a significant negative impact on the quality of fruit-producing trees, and in particular the lignin formation stimulates the development of stone cells in pear fruit. Reactive oxygen species (ROS) are essential for lignin polymerization. However, knowledge of the RBOH family, a key enzyme in ROS metabolism, remains unknown in most fruit trees. In this study, a total of 40 RBOHs were identified from five fruit-producing trees (Pyrus bretschneideri, Prunus persica, Citrus sinensis, Vitis vinifera, and Prunus mume), and 10 of these sequences came from Pyrus bretschneideri. Multiple sequence alignments revealed that all 10 PbRBOHs contained the NADPH_Ox domain and the six alpha-helical transmembrane domains (TM-I to TM-VI). Chromosome localization and interspecies phylogenetic tree analysis showed that 10 PbRBOHs irregularly distributed on 8 chromosomes and 3 PbRBOHs (PbRBOHA, PbRBOHB, and PbRBOHD) are closely related to known lignification-related RBOHs. Furthermore, hormone response pattern analysis showed that the transcription of PbRBOHs is regulated by SA, ABA and MeJA. Reverse transcription-quantitative real-time polymerase chain reaction (qRT-PCR) and transcriptome sequencing analysis showed that PbRBOHA, PbRBOHB, and PbRBOHD accumulated high transcript abundance in pear fruit, and the transcriptional trends of PbRBOHA and PbRBOHD was consistent with the change of stone cell content during fruit development. In addition, subcellular localization revealed that PbRBOHA and PbRBOHD are distributed on the plasma membrane. Combining the changes of apoplastic superoxide (O2.-) content and spatio-temporal expression analysis, these results indicate that PbRBOHA and PbRBOHD, which are candidate genes, may play an important role in ROS metabolism during the lignification of pear stone cells. This study not only provided insight into the molecular characteristics of the RBOH family in fruit-producing trees, but also lays the foundation for studying the role of ROS in plant lignification. SN - 2073-4409 UR - https://www.unboundmedicine.com/medline/citation/31146469/In_Silico_Genome_Wide_Analysis_of_Respiratory_Burst_Oxidase_Homolog__RBOH__Family_Genes_in_Five_Fruit_Producing_Trees_and_Potential_Functional_Analysis_on_Lignification_of_Stone_Cells_in_Chinese_White_Pear_ L2 - https://www.mdpi.com/resolver?pii=cells8060520 DB - PRIME DP - Unbound Medicine ER -