Tags

Type your tag names separated by a space and hit enter

Evaluation of the effect of experimentally induced cartilage defect and intra-articular hyaluronan on synovial fluid biomarkers in intercarpal joints of horses.
Acta Vet Scand. 2019 May 30; 61(1):24.AV

Abstract

BACKGROUND

Inflammatory and degenerative activity inside the joint can be studied in vivo by analysis of synovial fluid biomarkers. In addition to pro-inflammatory mediators, several anabolic and anti-inflammatory substances are produced during the disease process. They counteract the catabolic effects of the pro-inflammatory cytokines and thus diminish the cartilage damage. The response of synovial fluid biomarkers after intra-articular hyaluronan injection, alone or in combination with other substances, has been examined only in a few equine studies. The effects of hyaluronan on some pro-inflammatory mediators, such as prostaglandin E2, have been documented but especially the effects on synovial fluid anti-inflammatory mediators are less studied. In animal models hyaluronan has been demonstrated to reduce pain via protecting nociceptive nerve endings and by blocking pain receptor channels. However, the results obtained for pain-relief of human osteoarthritis are contradictory. The aim of the study was to measure the synovial fluid IL-1ra, PDGF-BB, TGF-β1 and TNF-α concentrations before and after surgically induced cartilage defect, and following intra-articular hyaluronan injection in horses. Eight Standardbred horses underwent bilateral arthroscopic surgeries of their intercarpal joints under general anaesthesia, and cartilage defect was created on the dorsal edge of the third carpal bone of one randomly selected intercarpal joint of each horse. Five days post-surgery, one randomly selected intercarpal joint was injected intra-articular with 3 mL HA (20 mg/mL).

RESULTS

Operation type had no significant effect on the synovial fluid IL-1ra, PDGF-BB, TGF-β1 and TNF-α concentrations but compared with baseline, synovial fluid IL-1ra and TNF-α concentrations increased. Intra-articular hyaluronan had no significant effect on the biomarker concentrations but a trend of mild improvement in the clinical signs of intra-articular inflammation was seen.

CONCLUSIONS

Creation of the cartilage defect and sham-operation lead to an increase of synovial fluid IL-1ra and TNF-α concentrations but changes in concentrations of anabolic growth factors TGF-β1 and PDGF-BB could not be documented 5 days after the arthroscopy. Intra-articular hyaluronan was well tolerated. Further research is needed to document possible treatment effects of intra-articular hyaluronan on the synovial fluid biomarkers of inflammation and cartilage metabolism.

Authors+Show Affiliations

Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 57, 00014, Helsinki, Finland. tytti.niemela@helsinki.fi.Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 57, 00014, Helsinki, Finland.Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Calle 65 No26-10, Manizales, Caldas, Colombia.Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Calle 65 No26-10, Manizales, Caldas, Colombia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31146775

Citation

Niemelä, Tytti Maaria, et al. "Evaluation of the Effect of Experimentally Induced Cartilage Defect and Intra-articular Hyaluronan On Synovial Fluid Biomarkers in Intercarpal Joints of Horses." Acta Veterinaria Scandinavica, vol. 61, no. 1, 2019, p. 24.
Niemelä TM, Tulamo RM, Carmona JU, et al. Evaluation of the effect of experimentally induced cartilage defect and intra-articular hyaluronan on synovial fluid biomarkers in intercarpal joints of horses. Acta Vet Scand. 2019;61(1):24.
Niemelä, T. M., Tulamo, R. M., Carmona, J. U., & López, C. (2019). Evaluation of the effect of experimentally induced cartilage defect and intra-articular hyaluronan on synovial fluid biomarkers in intercarpal joints of horses. Acta Veterinaria Scandinavica, 61(1), 24. https://doi.org/10.1186/s13028-019-0460-6
Niemelä TM, et al. Evaluation of the Effect of Experimentally Induced Cartilage Defect and Intra-articular Hyaluronan On Synovial Fluid Biomarkers in Intercarpal Joints of Horses. Acta Vet Scand. 2019 May 30;61(1):24. PubMed PMID: 31146775.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the effect of experimentally induced cartilage defect and intra-articular hyaluronan on synovial fluid biomarkers in intercarpal joints of horses. AU - Niemelä,Tytti Maaria, AU - Tulamo,Riitta-Mari, AU - Carmona,Jorge Uriel, AU - López,Catalina, Y1 - 2019/05/30/ PY - 2018/12/29/received PY - 2019/05/27/accepted PY - 2019/6/1/entrez PY - 2019/5/31/pubmed PY - 2019/7/11/medline KW - Cartilage defect KW - Experimental study KW - Hyaluronan KW - Interleukin-1 receptor antagonist KW - Non-animal stabilized hyaluronic acid (NASHA) KW - Platelet-derived growth factor BB KW - Synovitis KW - Transforming growth factor β1 KW - Tumour necrosis factor α SP - 24 EP - 24 JF - Acta veterinaria Scandinavica JO - Acta Vet. Scand. VL - 61 IS - 1 N2 - BACKGROUND: Inflammatory and degenerative activity inside the joint can be studied in vivo by analysis of synovial fluid biomarkers. In addition to pro-inflammatory mediators, several anabolic and anti-inflammatory substances are produced during the disease process. They counteract the catabolic effects of the pro-inflammatory cytokines and thus diminish the cartilage damage. The response of synovial fluid biomarkers after intra-articular hyaluronan injection, alone or in combination with other substances, has been examined only in a few equine studies. The effects of hyaluronan on some pro-inflammatory mediators, such as prostaglandin E2, have been documented but especially the effects on synovial fluid anti-inflammatory mediators are less studied. In animal models hyaluronan has been demonstrated to reduce pain via protecting nociceptive nerve endings and by blocking pain receptor channels. However, the results obtained for pain-relief of human osteoarthritis are contradictory. The aim of the study was to measure the synovial fluid IL-1ra, PDGF-BB, TGF-β1 and TNF-α concentrations before and after surgically induced cartilage defect, and following intra-articular hyaluronan injection in horses. Eight Standardbred horses underwent bilateral arthroscopic surgeries of their intercarpal joints under general anaesthesia, and cartilage defect was created on the dorsal edge of the third carpal bone of one randomly selected intercarpal joint of each horse. Five days post-surgery, one randomly selected intercarpal joint was injected intra-articular with 3 mL HA (20 mg/mL). RESULTS: Operation type had no significant effect on the synovial fluid IL-1ra, PDGF-BB, TGF-β1 and TNF-α concentrations but compared with baseline, synovial fluid IL-1ra and TNF-α concentrations increased. Intra-articular hyaluronan had no significant effect on the biomarker concentrations but a trend of mild improvement in the clinical signs of intra-articular inflammation was seen. CONCLUSIONS: Creation of the cartilage defect and sham-operation lead to an increase of synovial fluid IL-1ra and TNF-α concentrations but changes in concentrations of anabolic growth factors TGF-β1 and PDGF-BB could not be documented 5 days after the arthroscopy. Intra-articular hyaluronan was well tolerated. Further research is needed to document possible treatment effects of intra-articular hyaluronan on the synovial fluid biomarkers of inflammation and cartilage metabolism. SN - 1751-0147 UR - https://www.unboundmedicine.com/medline/citation/31146775/Evaluation_of_the_effect_of_experimentally_induced_cartilage_defect_and_intra_articular_hyaluronan_on_synovial_fluid_biomarkers_in_intercarpal_joints_of_horses_ L2 - https://actavetscand.biomedcentral.com/articles/10.1186/s13028-019-0460-6 DB - PRIME DP - Unbound Medicine ER -