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Doxycycline inhibits electric field-induced migration of non-small cell lung cancer (NSCLC) cells.
Sci Rep 2019; 9(1):8094SR

Abstract

Adenocarcinoma, large cell carcinoma and squamous cell carcinoma are the most commonly diagnosed subtypes of non-small cell lung cancers (NSCLC). Numerous lung cancer cell types have exhibited electrotaxis under direct current electric fields (dcEF). Physiological electric fields (EF) play key roles in cancer cell migration. In this study, we investigated electrotaxis of NSCLC cells, including human large cell lung carcinoma NCI-H460 and human lung squamous cell carcinoma NCI-H520 cells. Non-cancerous MRC-5 lung fibroblasts were included as a control. After dcEF stimulation, NCI-H460 and NCI-H520 cells, which both exhibit epithelial-like morphology, migrated towards the cathode, while MRC-5 cells, which have fibroblast-like morphology, migrated towards the anode. The effect of doxycycline, a common antibiotic, on electrotaxis of MRC-5, NCI-H460 and NCI-H520 cells was examined. Doxycycline enhanced the tested cells' motility but inhibited electrotaxis in the NSCLC cells without inhibiting non-cancerous MRC-5 cells. Based on our finding, further in-vivo studies could be devised to investigate the metastasis inhibition effect of doxycycline in an organism level.

Authors+Show Affiliations

Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan.Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan.Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan. Department of Engineering and System Science, National Tsing Hua University, Hsinchu, Taiwan. Nano Science and Technology Program, Taiwan International Graduate Program, Academia Sinica and National Tsing Hua University, Hsinchu, Taiwan.Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan.Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan. jycheng@gate.sinica.edu.tw. Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, Keelung, Taiwan. jycheng@gate.sinica.edu.tw. Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan. jycheng@gate.sinica.edu.tw. College of Engineering, Chang Gung Engineering, Taoyuan, Taiwan. jycheng@gate.sinica.edu.tw.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31147570

Citation

Chang, Hui-Fang, et al. "Doxycycline Inhibits Electric Field-induced Migration of Non-small Cell Lung Cancer (NSCLC) Cells." Scientific Reports, vol. 9, no. 1, 2019, p. 8094.
Chang HF, Cheng HT, Chen HY, et al. Doxycycline inhibits electric field-induced migration of non-small cell lung cancer (NSCLC) cells. Sci Rep. 2019;9(1):8094.
Chang, H. F., Cheng, H. T., Chen, H. Y., Yeung, W. K., & Cheng, J. Y. (2019). Doxycycline inhibits electric field-induced migration of non-small cell lung cancer (NSCLC) cells. Scientific Reports, 9(1), p. 8094. doi:10.1038/s41598-019-44505-8.
Chang HF, et al. Doxycycline Inhibits Electric Field-induced Migration of Non-small Cell Lung Cancer (NSCLC) Cells. Sci Rep. 2019 May 30;9(1):8094. PubMed PMID: 31147570.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Doxycycline inhibits electric field-induced migration of non-small cell lung cancer (NSCLC) cells. AU - Chang,Hui-Fang, AU - Cheng,Hung-Tien, AU - Chen,Huai-Yi, AU - Yeung,Wing Kiu, AU - Cheng,Ji-Yen, Y1 - 2019/05/30/ PY - 2018/12/27/received PY - 2019/05/15/accepted PY - 2019/6/1/entrez PY - 2019/5/31/pubmed PY - 2019/5/31/medline SP - 8094 EP - 8094 JF - Scientific reports JO - Sci Rep VL - 9 IS - 1 N2 - Adenocarcinoma, large cell carcinoma and squamous cell carcinoma are the most commonly diagnosed subtypes of non-small cell lung cancers (NSCLC). Numerous lung cancer cell types have exhibited electrotaxis under direct current electric fields (dcEF). Physiological electric fields (EF) play key roles in cancer cell migration. In this study, we investigated electrotaxis of NSCLC cells, including human large cell lung carcinoma NCI-H460 and human lung squamous cell carcinoma NCI-H520 cells. Non-cancerous MRC-5 lung fibroblasts were included as a control. After dcEF stimulation, NCI-H460 and NCI-H520 cells, which both exhibit epithelial-like morphology, migrated towards the cathode, while MRC-5 cells, which have fibroblast-like morphology, migrated towards the anode. The effect of doxycycline, a common antibiotic, on electrotaxis of MRC-5, NCI-H460 and NCI-H520 cells was examined. Doxycycline enhanced the tested cells' motility but inhibited electrotaxis in the NSCLC cells without inhibiting non-cancerous MRC-5 cells. Based on our finding, further in-vivo studies could be devised to investigate the metastasis inhibition effect of doxycycline in an organism level. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/31147570/Doxycycline_inhibits_electric_field-induced_migration_of_non-small_cell_lung_cancer_(NSCLC)_cells L2 - http://dx.doi.org/10.1038/s41598-019-44505-8 DB - PRIME DP - Unbound Medicine ER -