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The salivary transcriptome of Limnobdella mexicana (Annelida: Clitellata: Praobdellidae) and orthology determination of major leech anticoagulants.
Parasitology. 2019 09; 146(10):1338-1346.P

Abstract

Bloodfeeding requires several adaptations that allow the parasite to feed efficiently. Leeches and other hematophagous animals have developed different mechanisms to inhibit hemostasis, one of the main barriers imposed by their hosts. Limnobdella mexicana is a member of the leech family Praobdellidae, a family of host generalists known for their preference to attach on mucosal membranes of mammals, such as those in nasopharyngeal cavities, bladders and ocular orbits. Previous studies have hypothesized a positive relationship between diversity of anticoagulants and diversity of hosts in bloodfeeding leeches. However, orthology determination of putative anticoagulants and the lack of standardization of sequencing effort and method hinder comparisons between publicly available transcriptomes generated in different laboratories. In the present study, we examine the first transcriptome of a praobdellid leech and identify 15 putative anticoagulants using a phylogeny-based inference approach, amino-acid conservation, Pfam domains and BLAST searches. Our phylogenetic analyses suggest that the ancestral leech was able to inhibit factor Xa and that some hirudins that have been reported in previous studies on leech anticoagulants may not be orthologous with the archetypal hirudin.

Authors+Show Affiliations

Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, ON M5S 2C6, Canada. Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks Street, Toronto, ON M5S 2B4, Canada.Laboratorio de Helmintología, Instituto de Biología, Universidad Nacional Autónoma de México, Circuito Zona Deportiva 53, Ciudad Universitaria, 04510 Coyoacan Ciudad de México, México.Museum of Comparative Zoology & Department of Organismic and Evolutionary Biology, Harvard University, Cambridge MA, 02138, USA.Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, ON M5S 2C6, Canada. Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks Street, Toronto, ON M5S 2B4, Canada.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31148528

Citation

Iwama, Rafael, et al. "The Salivary Transcriptome of Limnobdella Mexicana (Annelida: Clitellata: Praobdellidae) and Orthology Determination of Major Leech Anticoagulants." Parasitology, vol. 146, no. 10, 2019, pp. 1338-1346.
Iwama R, Oceguera-Figueroa A, Giribet G, et al. The salivary transcriptome of Limnobdella mexicana (Annelida: Clitellata: Praobdellidae) and orthology determination of major leech anticoagulants. Parasitology. 2019;146(10):1338-1346.
Iwama, R., Oceguera-Figueroa, A., Giribet, G., & Kvist, S. (2019). The salivary transcriptome of Limnobdella mexicana (Annelida: Clitellata: Praobdellidae) and orthology determination of major leech anticoagulants. Parasitology, 146(10), 1338-1346. https://doi.org/10.1017/S0031182019000593
Iwama R, et al. The Salivary Transcriptome of Limnobdella Mexicana (Annelida: Clitellata: Praobdellidae) and Orthology Determination of Major Leech Anticoagulants. Parasitology. 2019;146(10):1338-1346. PubMed PMID: 31148528.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The salivary transcriptome of Limnobdella mexicana (Annelida: Clitellata: Praobdellidae) and orthology determination of major leech anticoagulants. AU - Iwama,Rafael, AU - Oceguera-Figueroa,Alejandro, AU - Giribet,Gonzalo, AU - Kvist,Sebastian, Y1 - 2019/06/27/ PY - 2019/6/1/pubmed PY - 2020/4/29/medline PY - 2019/6/1/entrez KW - Anticoagulants KW - Praobdellidae KW - antistasin KW - bloodfeeding KW - hirudin KW - transcriptomics SP - 1338 EP - 1346 JF - Parasitology JO - Parasitology VL - 146 IS - 10 N2 - Bloodfeeding requires several adaptations that allow the parasite to feed efficiently. Leeches and other hematophagous animals have developed different mechanisms to inhibit hemostasis, one of the main barriers imposed by their hosts. Limnobdella mexicana is a member of the leech family Praobdellidae, a family of host generalists known for their preference to attach on mucosal membranes of mammals, such as those in nasopharyngeal cavities, bladders and ocular orbits. Previous studies have hypothesized a positive relationship between diversity of anticoagulants and diversity of hosts in bloodfeeding leeches. However, orthology determination of putative anticoagulants and the lack of standardization of sequencing effort and method hinder comparisons between publicly available transcriptomes generated in different laboratories. In the present study, we examine the first transcriptome of a praobdellid leech and identify 15 putative anticoagulants using a phylogeny-based inference approach, amino-acid conservation, Pfam domains and BLAST searches. Our phylogenetic analyses suggest that the ancestral leech was able to inhibit factor Xa and that some hirudins that have been reported in previous studies on leech anticoagulants may not be orthologous with the archetypal hirudin. SN - 1469-8161 UR - https://www.unboundmedicine.com/medline/citation/31148528/The_salivary_transcriptome_of_Limnobdella_mexicana__Annelida:_Clitellata:_Praobdellidae__and_orthology_determination_of_major_leech_anticoagulants_ L2 - https://www.cambridge.org/core/product/identifier/S0031182019000593/type/journal_article DB - PRIME DP - Unbound Medicine ER -