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Inhibition of NADP-malic enzyme activity by H2 S and NO in sweet pepper (Capsicum annuum L.) fruits.
Physiol Plant. 2020 Feb; 168(2):278-288.PP

Abstract

NADPH is an essential cofactor in many physiological processes. Fruit ripening is caused by multiple biochemical pathways in which, reactive oxygen and nitrogen species (ROS/RNS) metabolism is involved. Previous studies have demonstrated the differential modulation of nitric oxide (NO) and hydrogen sulfide (H2 S) content during sweet pepper (Capsicum annuum L.) fruit ripening, both of which regulate NADP-isocitrate dehydrogenase activity. To gain a deeper understanding of the potential functions of other NADPH-generating components, we analyzed glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH), which are involved in the oxidative phase of the pentose phosphate pathway (OxPPP) and NADP-malic enzyme (NADP-ME). During fruit ripening, G6PDH activity diminished by 38%, while 6PGDH and NADP-ME activity increased 1.5- and 2.6-fold, respectively. To better understand the potential regulation of these NADP-dehydrogenases by H2 S, we obtained a 50-75% ammonium-sulfate-enriched protein fraction containing these proteins. With the aid of in vitro assays, in the presence of H2 S, we observed that, while NADP-ME activity was inhibited by up to 29-32% using 2 and 5 mM Na2 S as H2 S donor, G6PDH and 6PGDH activities were unaffected. On the other hand, NO donors, S-nitrosocyteine (CysNO) and DETA NONOate also inhibited NADP-ME activity by 35%. These findings suggest that both NADP-ME and 6PGDH play an important role in maintaining the supply of NADPH during pepper fruit ripening and that H2 S and NO partially modulate the NADPH-generating system.

Authors+Show Affiliations

Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), 18008, Granada, Spain.Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), 18008, Granada, Spain.Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), 18008, Granada, Spain.Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), 18008, Granada, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31152557

Citation

Muñoz-Vargas, María A., et al. "Inhibition of NADP-malic Enzyme Activity By H2 S and NO in Sweet Pepper (Capsicum Annuum L.) Fruits." Physiologia Plantarum, vol. 168, no. 2, 2020, pp. 278-288.
Muñoz-Vargas MA, González-Gordo S, Palma JM, et al. Inhibition of NADP-malic enzyme activity by H2 S and NO in sweet pepper (Capsicum annuum L.) fruits. Physiol Plant. 2020;168(2):278-288.
Muñoz-Vargas, M. A., González-Gordo, S., Palma, J. M., & Corpas, F. J. (2020). Inhibition of NADP-malic enzyme activity by H2 S and NO in sweet pepper (Capsicum annuum L.) fruits. Physiologia Plantarum, 168(2), 278-288. https://doi.org/10.1111/ppl.13000
Muñoz-Vargas MA, et al. Inhibition of NADP-malic Enzyme Activity By H2 S and NO in Sweet Pepper (Capsicum Annuum L.) Fruits. Physiol Plant. 2020;168(2):278-288. PubMed PMID: 31152557.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of NADP-malic enzyme activity by H2 S and NO in sweet pepper (Capsicum annuum L.) fruits. AU - Muñoz-Vargas,María A, AU - González-Gordo,Salvador, AU - Palma,José M, AU - Corpas,Francisco J, Y1 - 2019/06/25/ PY - 2019/04/20/received PY - 2019/05/21/revised PY - 2019/05/28/accepted PY - 2019/6/4/pubmed PY - 2020/3/12/medline PY - 2019/6/2/entrez SP - 278 EP - 288 JF - Physiologia plantarum JO - Physiol Plant VL - 168 IS - 2 N2 - NADPH is an essential cofactor in many physiological processes. Fruit ripening is caused by multiple biochemical pathways in which, reactive oxygen and nitrogen species (ROS/RNS) metabolism is involved. Previous studies have demonstrated the differential modulation of nitric oxide (NO) and hydrogen sulfide (H2 S) content during sweet pepper (Capsicum annuum L.) fruit ripening, both of which regulate NADP-isocitrate dehydrogenase activity. To gain a deeper understanding of the potential functions of other NADPH-generating components, we analyzed glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH), which are involved in the oxidative phase of the pentose phosphate pathway (OxPPP) and NADP-malic enzyme (NADP-ME). During fruit ripening, G6PDH activity diminished by 38%, while 6PGDH and NADP-ME activity increased 1.5- and 2.6-fold, respectively. To better understand the potential regulation of these NADP-dehydrogenases by H2 S, we obtained a 50-75% ammonium-sulfate-enriched protein fraction containing these proteins. With the aid of in vitro assays, in the presence of H2 S, we observed that, while NADP-ME activity was inhibited by up to 29-32% using 2 and 5 mM Na2 S as H2 S donor, G6PDH and 6PGDH activities were unaffected. On the other hand, NO donors, S-nitrosocyteine (CysNO) and DETA NONOate also inhibited NADP-ME activity by 35%. These findings suggest that both NADP-ME and 6PGDH play an important role in maintaining the supply of NADPH during pepper fruit ripening and that H2 S and NO partially modulate the NADPH-generating system. SN - 1399-3054 UR - https://www.unboundmedicine.com/medline/citation/31152557/Inhibition_of_NADP_malic_enzyme_activity_by_H2_S_and_NO_in_sweet_pepper__Capsicum_annuum_L___fruits_ L2 - https://doi.org/10.1111/ppl.13000 DB - PRIME DP - Unbound Medicine ER -