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Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite scaffolds via suspension, two-nozzle and multilayer electrospinning for skin tissue engineering.
Int J Biol Macromol 2019; 135:530-543IJ

Abstract

This work investigates the incorporation of the Calendula officinalis (C. officinalis) extract in electrospun fiber scaffolds composed of poly (ε-caprolactone) (PCL), Zein and gum arabic (GA). Three methods of electrospinning were used: suspension electrospinning, in which C. officinalis extract was directly added in the PCL/Zein/GA solution, two-nozzle electrospinning, in which hybrid PCL/Zein/GA and PCL/C. officinalis nanofibrous layers were prepared by two syringes and multilayer electrospinning, in which layer-by-layer scaffold was fabricated of PCL/Zein/GA and PCL/C.officinalis nanofibrous mats. SEM micrographs of fabricated scaffolds depicted beadless nanofibers with interconnected pores. The PCL/Zein/GA/C.officinalis scaffolds possess good hydrophilicity with high porosity (about 80%) and also exhibited desirable mechanical properties and suitable degradability for skin tissue engineering. Multilayer produced scaffold showed more tensile strength than other C. officinalis-loaded PCL/Zein/GA scaffolds. In vitro C.officinalis release exposed gradual and sustained release behavior for fabricated scaffold by multilayer electrospinning. The results of MTT analysis and SEM images confirmed that PCL/Zein/GA/C.officinalis nanocomposite scaffold had favorable proliferation and adhesion against fibroblast cell as compared to PCL/Zein/GA scaffold for regenerating skin. The C. officinalis-loaded PCL/Zein/GA scaffold indicated better antibacterial properties and biocompatibility than PCL/Zein/GA scaffold. The results confirmed that C. officinalis-loaded PCL/Zein/GA nanocomposite scaffolds would be desirable biomaterial for skin regeneration.

Authors+Show Affiliations

Department of Textile Engineering, Faculty of Engineering, University of Guilan, Rasht, Iran.Department of Textile Engineering, Faculty of Engineering, University of Guilan, Rasht, Iran. Electronic address: j.mokhtari@guilan.ac.ir.Department of Textile Engineering, Faculty of Engineering, University of Guilan, Rasht, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31152839

Citation

Pedram Rad, Zahra, et al. "Calendula Officinalis extract/PCL/Zein/Gum Arabic Nanofibrous Bio-composite Scaffolds Via Suspension, Two-nozzle and Multilayer Electrospinning for Skin Tissue Engineering." International Journal of Biological Macromolecules, vol. 135, 2019, pp. 530-543.
Pedram Rad Z, Mokhtari J, Abbasi M. Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite scaffolds via suspension, two-nozzle and multilayer electrospinning for skin tissue engineering. Int J Biol Macromol. 2019;135:530-543.
Pedram Rad, Z., Mokhtari, J., & Abbasi, M. (2019). Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite scaffolds via suspension, two-nozzle and multilayer electrospinning for skin tissue engineering. International Journal of Biological Macromolecules, 135, pp. 530-543. doi:10.1016/j.ijbiomac.2019.05.204.
Pedram Rad Z, Mokhtari J, Abbasi M. Calendula Officinalis extract/PCL/Zein/Gum Arabic Nanofibrous Bio-composite Scaffolds Via Suspension, Two-nozzle and Multilayer Electrospinning for Skin Tissue Engineering. Int J Biol Macromol. 2019 Aug 15;135:530-543. PubMed PMID: 31152839.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite scaffolds via suspension, two-nozzle and multilayer electrospinning for skin tissue engineering. AU - Pedram Rad,Zahra, AU - Mokhtari,Javad, AU - Abbasi,Marjan, Y1 - 2019/05/29/ PY - 2019/05/01/received PY - 2019/05/27/revised PY - 2019/05/27/accepted PY - 2019/6/4/pubmed PY - 2019/12/20/medline PY - 2019/6/2/entrez KW - Calendula officinalis KW - Electrospinning KW - Gum Arabic KW - Polycaprolactone KW - Skin tissue engineering KW - Zein SP - 530 EP - 543 JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. VL - 135 N2 - This work investigates the incorporation of the Calendula officinalis (C. officinalis) extract in electrospun fiber scaffolds composed of poly (ε-caprolactone) (PCL), Zein and gum arabic (GA). Three methods of electrospinning were used: suspension electrospinning, in which C. officinalis extract was directly added in the PCL/Zein/GA solution, two-nozzle electrospinning, in which hybrid PCL/Zein/GA and PCL/C. officinalis nanofibrous layers were prepared by two syringes and multilayer electrospinning, in which layer-by-layer scaffold was fabricated of PCL/Zein/GA and PCL/C.officinalis nanofibrous mats. SEM micrographs of fabricated scaffolds depicted beadless nanofibers with interconnected pores. The PCL/Zein/GA/C.officinalis scaffolds possess good hydrophilicity with high porosity (about 80%) and also exhibited desirable mechanical properties and suitable degradability for skin tissue engineering. Multilayer produced scaffold showed more tensile strength than other C. officinalis-loaded PCL/Zein/GA scaffolds. In vitro C.officinalis release exposed gradual and sustained release behavior for fabricated scaffold by multilayer electrospinning. The results of MTT analysis and SEM images confirmed that PCL/Zein/GA/C.officinalis nanocomposite scaffold had favorable proliferation and adhesion against fibroblast cell as compared to PCL/Zein/GA scaffold for regenerating skin. The C. officinalis-loaded PCL/Zein/GA scaffold indicated better antibacterial properties and biocompatibility than PCL/Zein/GA scaffold. The results confirmed that C. officinalis-loaded PCL/Zein/GA nanocomposite scaffolds would be desirable biomaterial for skin regeneration. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/31152839/Calendula_officinalis_extract/PCL/Zein/Gum_arabic_nanofibrous_bio_composite_scaffolds_via_suspension_two_nozzle_and_multilayer_electrospinning_for_skin_tissue_engineering_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(19)33227-1 DB - PRIME DP - Unbound Medicine ER -