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cVEMP correlated with imbalance in a mouse model of vestibular disorder.

Abstract

BACKGROUND

Cervical vestibular evoked myogenic potential (cVEMP) testing is a strong tool that enables objective determination of balance functions in humans. However, it remains unknown whether cVEMP correctly expresses vestibular disorder in mice.

OBJECTIVE

In this study, correlations of cVEMP with scores for balance-related behavior tests including rotarod, beam, and air-righting reflex tests were determined in ICR mice with vestibular disorder induced by 3,3'-iminodipropiontrile (IDPN) as a mouse model of vestibular disorder.

METHODS

Male ICR mice at 4 weeks of age were orally administered IDPN in saline (28 mmol/kg body weight) once. Rotarod, beam crossing, and air-righting reflex tests were performed before and 3-4 days after oral exposure one time to IDPN to determine balance functions. The saccule and utricles were labeled with fluorescein phalloidin. cVEMP measurements were performed for mice in the control and IDPN groups. Finally, the correlations between the scores of behavior tests and the amplitude or latency of cVEMP were determined with Spearman's rank correlation coefficient. Two-tailed Student's t test and Welch's t test were used to determine a significant difference between the two groups. A difference with p < 0.05 was considered to indicate statistical significance.

RESULTS

After oral administration of IDPN at 28 mmol/kg, scores of the rotarod, beam, and air-righting reflex tests in the IDPN group were significantly lower than those in the control group. The numbers of hair cells in the saccule, utricle, and cupula were decreased in the IDPN group. cVEMP in the IDPN group was significantly decreased in amplitude and increased in latency compared to those in the control group. cVEMP amplitude had significant correlations with the numbers of hair cells as well as scores for all of the behavior tests in mice.

CONCLUSIONS

This study demonstrated impaired cVEMP and correlations of cVEMP with imbalance determined by behavior tests in a mouse model of vestibular disorder.

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  • Authors+Show Affiliations

    ,

    Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi, 466-8550, Japan.

    ,

    Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi, 466-8550, Japan.

    ,

    Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi, 466-8550, Japan.

    ,

    Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi, 466-8550, Japan.

    ,

    Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi, 466-8550, Japan.

    Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi, 466-8550, Japan. katomasa@med.nagoya-u.ac.jp.

    Source

    MeSH

    Animals
    Behavior, Animal
    Disease Models, Animal
    Hair Cells, Vestibular
    Male
    Mice
    Mice, Inbred ICR
    Nitriles
    Postural Balance
    Saccule and Utricle
    Sensation Disorders
    Vestibular Diseases
    Vestibular Evoked Myogenic Potentials
    Vestibular Function Tests

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31153359

    Citation

    Negishi-Oshino, Reina, et al. "CVEMP Correlated With Imbalance in a Mouse Model of Vestibular Disorder." Environmental Health and Preventive Medicine, vol. 24, no. 1, 2019, p. 39.
    Negishi-Oshino R, Ohgami N, He T, et al. CVEMP correlated with imbalance in a mouse model of vestibular disorder. Environ Health Prev Med. 2019;24(1):39.
    Negishi-Oshino, R., Ohgami, N., He, T., Ohgami, K., Li, X., & Kato, M. (2019). CVEMP correlated with imbalance in a mouse model of vestibular disorder. Environmental Health and Preventive Medicine, 24(1), p. 39. doi:10.1186/s12199-019-0794-8.
    Negishi-Oshino R, et al. CVEMP Correlated With Imbalance in a Mouse Model of Vestibular Disorder. Environ Health Prev Med. 2019 Jun 1;24(1):39. PubMed PMID: 31153359.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - cVEMP correlated with imbalance in a mouse model of vestibular disorder. AU - Negishi-Oshino,Reina, AU - Ohgami,Nobutaka, AU - He,Tingchao, AU - Ohgami,Kyoko, AU - Li,Xiang, AU - Kato,Masashi, Y1 - 2019/06/01/ PY - 2019/02/18/received PY - 2019/05/14/accepted PY - 2019/6/3/entrez PY - 2019/6/4/pubmed PY - 2019/6/15/medline KW - Balance KW - Hair cells KW - IDPN KW - Vestibule KW - cVEMP SP - 39 EP - 39 JF - Environmental health and preventive medicine JO - Environ Health Prev Med VL - 24 IS - 1 N2 - BACKGROUND: Cervical vestibular evoked myogenic potential (cVEMP) testing is a strong tool that enables objective determination of balance functions in humans. However, it remains unknown whether cVEMP correctly expresses vestibular disorder in mice. OBJECTIVE: In this study, correlations of cVEMP with scores for balance-related behavior tests including rotarod, beam, and air-righting reflex tests were determined in ICR mice with vestibular disorder induced by 3,3'-iminodipropiontrile (IDPN) as a mouse model of vestibular disorder. METHODS: Male ICR mice at 4 weeks of age were orally administered IDPN in saline (28 mmol/kg body weight) once. Rotarod, beam crossing, and air-righting reflex tests were performed before and 3-4 days after oral exposure one time to IDPN to determine balance functions. The saccule and utricles were labeled with fluorescein phalloidin. cVEMP measurements were performed for mice in the control and IDPN groups. Finally, the correlations between the scores of behavior tests and the amplitude or latency of cVEMP were determined with Spearman's rank correlation coefficient. Two-tailed Student's t test and Welch's t test were used to determine a significant difference between the two groups. A difference with p < 0.05 was considered to indicate statistical significance. RESULTS: After oral administration of IDPN at 28 mmol/kg, scores of the rotarod, beam, and air-righting reflex tests in the IDPN group were significantly lower than those in the control group. The numbers of hair cells in the saccule, utricle, and cupula were decreased in the IDPN group. cVEMP in the IDPN group was significantly decreased in amplitude and increased in latency compared to those in the control group. cVEMP amplitude had significant correlations with the numbers of hair cells as well as scores for all of the behavior tests in mice. CONCLUSIONS: This study demonstrated impaired cVEMP and correlations of cVEMP with imbalance determined by behavior tests in a mouse model of vestibular disorder. SN - 1347-4715 UR - https://www.unboundmedicine.com/medline/citation/31153359/cVEMP_correlated_with_imbalance_in_a_mouse_model_of_vestibular_disorder L2 - https://environhealthprevmed.biomedcentral.com/articles/10.1186/s12199-019-0794-8 DB - PRIME DP - Unbound Medicine ER -