TY - JOUR T1 - Fiber optic surface plasmon resonance biosensor for detection of PDGF-BB in serum based on self-assembled aptamer and antifouling peptide monolayer. AU - Qian,Husun, AU - Huang,Yu, AU - Duan,Xiaolei, AU - Wei,Xiaotong, AU - Fan,Yunpeng, AU - Gan,Delu, AU - Yue,Shujun, AU - Cheng,Wei, AU - Chen,Tingmei, Y1 - 2019/05/25/ PY - 2019/03/21/received PY - 2019/05/21/revised PY - 2019/05/23/accepted PY - 2019/6/4/pubmed PY - 2019/12/18/medline PY - 2019/6/3/entrez KW - Antifouling peptide KW - Biosensor KW - Fiber optic KW - PDGF-BB KW - Surface plasmon resonance SP - 111350 EP - 111350 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 140 N2 - Herein, a home-build fiber optic surface plasmon resonance (FO-SPR) biosensing platform has been developed for highly sensitive detection of platelet-derived growth factor (PDGF-BB) based aptamer-functionalized AuNPs for signal enhancement. In this biosensor, the PDGF-BB aptamer was used to specifically capture PDGF-BB, and the antifouling peptide demonstrated great ability for resisting non-specific adsorption. After a sandwich reaction, the aptamer, PDGF-BB and aptamer-functionalized AuNPs complexes were formed on the fiber optic (FO) probe surface to significantly amplify FO-SPR signal. This method exhibited a broad detection range from 1 to 1000 pM of PDGF-BB and a low detection limit of 0.35 pM. Moreover, this biosensor was successfully applied to the detection of PDGF-BB in 10% human serum samples without suffering from serious interference owing to the excellent antifouling property of the peptide. Thus, this developed FO-SPR biosensor could be a potential alternative device for proteins determination, even as a point-of-care diagnostic tool (POCT) in clinical application. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/31154255/Fiber_optic_surface_plasmon_resonance_biosensor_for_detection_of_PDGF_BB_in_serum_based_on_self_assembled_aptamer_and_antifouling_peptide_monolayer_ DB - PRIME DP - Unbound Medicine ER -