Tags

Type your tag names separated by a space and hit enter

Unveiling early cortical and subcortical neuronal degeneration in ALS mice by ultra-high field diffusion MRI.

Abstract

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease primarily characterized by the progressive impairment of motor functions. However, a significant portion of affected patients develops severe cognitive dysfunction, developing a widespread white (WM) and gray matter (GM) microstructural impairment. The objective of this study is to determine if Gaussian and non-Gaussian diffusion models gathered by ultra-high field diffusion MRI (UHFD-MRI) are an appropriate tool to detect early structural changes in brain white and gray matter in a preclinical model of ALS. ALS brains (G93A-SOD1mice) were scanned in a 16.7 T magnet. Diffusion tensor imaging (DTI) and neurite orientation dispersion and density imaging (NODDI) have shown presymptomatic decrease in axonal organization by Fractional Anisotropy (FA) and neurite content by Intracellular Volume Fraction (ICVF) across deep WM (corpus callosum) as well as superficial (cortex) and deep (hippocampus) GM. Additional diffusion kurtosis imaging (DKI) analysis demonstrated broader and earlier GM reductions in mean kurtosis (MK), possibly related to the decrease in neuronal complexity. Histological validation was obtained by an ALS fluorescent mice reporter (YFP, G93A-SOD1 mice). The combination of DTI, NODDI, and DKI models have proved to provide a more complete assessment of the early microstructural changes in the ALS brain, particularly in areas associated with high cognitive functions. This comprehensive approach should be considered as a valuable tool for the early detection of neuroimaging markers.

Authors+Show Affiliations

Department of Bioengineering, University of Illinois at Chicago , Chicago , IL , USA.Department of Biochemistry and Molecular Biology, National High Magnetic Field Laboratory, University of Florida , Gainesville , FL , USA.Department of Pathology, Feinberg School of Medicine, Northwestern University , Chicago , IL , USA.Institute for Physiology, Molecular Biology and Neurosciences (IFIBYNE CONICET-UBA), Buenos Aires , Argentina.Department of Biomedical Engineering, University of Florida , Gainesville , FL , USA , and.Department of Microbiology, University of Florida , Gainesville , FL , USA.Institute for Physiology, Molecular Biology and Neurosciences (IFIBYNE CONICET-UBA), Buenos Aires , Argentina.Department of Pathology, Feinberg School of Medicine, Northwestern University , Chicago , IL , USA.Department of Biochemistry and Molecular Biology, National High Magnetic Field Laboratory, University of Florida , Gainesville , FL , USA.Department of Bioengineering, University of Illinois at Chicago , Chicago , IL , USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

31159586

Citation

Gatto, Rodolfo G., et al. "Unveiling Early Cortical and Subcortical Neuronal Degeneration in ALS Mice By Ultra-high Field Diffusion MRI." Amyotrophic Lateral Sclerosis & Frontotemporal Degeneration, vol. 20, no. 7-8, 2019, pp. 549-561.
Gatto RG, Amin M, Finkielsztein A, et al. Unveiling early cortical and subcortical neuronal degeneration in ALS mice by ultra-high field diffusion MRI. Amyotroph Lateral Scler Frontotemporal Degener. 2019;20(7-8):549-561.
Gatto, R. G., Amin, M., Finkielsztein, A., Weissmann, C., Barrett, T., Lamoutte, C., Uchitel, O., Sumagin, R., Mareci, T. H., & Magin, R. L. (2019). Unveiling early cortical and subcortical neuronal degeneration in ALS mice by ultra-high field diffusion MRI. Amyotrophic Lateral Sclerosis & Frontotemporal Degeneration, 20(7-8), 549-561. https://doi.org/10.1080/21678421.2019.1620285
Gatto RG, et al. Unveiling Early Cortical and Subcortical Neuronal Degeneration in ALS Mice By Ultra-high Field Diffusion MRI. Amyotroph Lateral Scler Frontotemporal Degener. 2019;20(7-8):549-561. PubMed PMID: 31159586.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Unveiling early cortical and subcortical neuronal degeneration in ALS mice by ultra-high field diffusion MRI. AU - Gatto,Rodolfo G, AU - Amin,Manish, AU - Finkielsztein,Ariel, AU - Weissmann,Carina, AU - Barrett,Thomas, AU - Lamoutte,Caroline, AU - Uchitel,Osvaldo, AU - Sumagin,Ronen, AU - Mareci,Thomas H, AU - Magin,Richard L, Y1 - 2019/06/03/ PY - 2019/6/5/pubmed PY - 2020/7/31/medline PY - 2019/6/5/entrez KW - SP - 549 EP - 561 JF - Amyotrophic lateral sclerosis & frontotemporal degeneration JO - Amyotroph Lateral Scler Frontotemporal Degener VL - 20 IS - 7-8 N2 - Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease primarily characterized by the progressive impairment of motor functions. However, a significant portion of affected patients develops severe cognitive dysfunction, developing a widespread white (WM) and gray matter (GM) microstructural impairment. The objective of this study is to determine if Gaussian and non-Gaussian diffusion models gathered by ultra-high field diffusion MRI (UHFD-MRI) are an appropriate tool to detect early structural changes in brain white and gray matter in a preclinical model of ALS. ALS brains (G93A-SOD1mice) were scanned in a 16.7 T magnet. Diffusion tensor imaging (DTI) and neurite orientation dispersion and density imaging (NODDI) have shown presymptomatic decrease in axonal organization by Fractional Anisotropy (FA) and neurite content by Intracellular Volume Fraction (ICVF) across deep WM (corpus callosum) as well as superficial (cortex) and deep (hippocampus) GM. Additional diffusion kurtosis imaging (DKI) analysis demonstrated broader and earlier GM reductions in mean kurtosis (MK), possibly related to the decrease in neuronal complexity. Histological validation was obtained by an ALS fluorescent mice reporter (YFP, G93A-SOD1 mice). The combination of DTI, NODDI, and DKI models have proved to provide a more complete assessment of the early microstructural changes in the ALS brain, particularly in areas associated with high cognitive functions. This comprehensive approach should be considered as a valuable tool for the early detection of neuroimaging markers. SN - 2167-9223 UR - https://www.unboundmedicine.com/medline/citation/31159586/Unveiling_early_cortical_and_subcortical_neuronal_degeneration_in_ALS_mice_by_ultra_high_field_diffusion_MRI_ L2 - http://www.tandfonline.com/doi/full/10.1080/21678421.2019.1620285 DB - PRIME DP - Unbound Medicine ER -