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Electrochemical detection of Salmonella using an invA genosensor on polypyrrole-reduced graphene oxide modified glassy carbon electrode and AuNPs-horseradish peroxidase-streptavidin as nanotag.
Anal Chim Acta. 2019 Oct 03; 1074:80-88.AC

Abstract

A rapid and sensitive electrochemical biosensor was constructed to detect Salmonella using invA gene biosensor. The biosensing was based on polyrrole-reduced graphene oxide (PPy-rGO) nanocomposite modified glassy carbon electrode (GCE) and signal amplification with horseradish peroxidase-streptavidin biofunctionalized gold nanoparticles (AuNPs-HRP-SA). PPy-rGO was prepared at 60 °C by chemical reduction of PPy-functionalized graphene oxide (PPy-GO) that was synthesized by in situ polymerization at room temperature. The detection signal was amplified via enzymatic reduction of H2O2 in the presence of hydroquinone (HQ) using AuNPs-HRP-SA as nanotag. Under optimal conditions, the differential pulse voltametric (DPV) signal from the biosensor was linearly related to the logarithm of target invA gene concentrations from 1.0 × 10-16 to 1.0 × 10-10 M, and the limit of detection (LOD) was 4.7 × 10-17 M. The biosensor can also detect Salmonella in the range of 9.6 to 9.6 × 104 CFU mL-1, with LOD of 8.07 CFU mL-1. The biosensor showed good regeneration ability, acceptable selectivity, repeatability and stability, which bode well as an alternative method for Salmonella screening.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Ye Y
School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China.
Yan W
School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China.
Liu Y
School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China.
He S
School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China.
Cao X
School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China. Electronic address: xiaodongcao@hfut.edu.cn.
Xu X
College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing, 211816, China.
Zheng H
Technology Center of Anhui Entry-Exit Inspection and Quarantine Bureau, Hefei, 230032, China.
Gunasekaran S
Department of Biological Systems Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA. Electronic address: guna@wisc.edu.

MeSH

Bacterial ProteinsBacterial Typing TechniquesBiosensing TechniquesCarbonDNA, BacterialElectrochemical TechniquesElectrodesEnzymes, ImmobilizedGoldGraphiteHorseradish PeroxidaseHydrogen PeroxideHydroquinonesLimit of DetectionMetal NanoparticlesNanocompositesNucleic Acid HybridizationOxidation-ReductionPolymersPyrrolesSalmonellaStreptavidin

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31159942

Citation

Ye, Yongkang, et al. "Electrochemical Detection of Salmonella Using an invA Genosensor On Polypyrrole-reduced Graphene Oxide Modified Glassy Carbon Electrode and AuNPs-horseradish Peroxidase-streptavidin as Nanotag." Analytica Chimica Acta, vol. 1074, 2019, pp. 80-88.
Ye Y, Yan W, Liu Y, et al. Electrochemical detection of Salmonella using an invA genosensor on polypyrrole-reduced graphene oxide modified glassy carbon electrode and AuNPs-horseradish peroxidase-streptavidin as nanotag. Anal Chim Acta. 2019;1074:80-88.
Ye, Y., Yan, W., Liu, Y., He, S., Cao, X., Xu, X., Zheng, H., & Gunasekaran, S. (2019). Electrochemical detection of Salmonella using an invA genosensor on polypyrrole-reduced graphene oxide modified glassy carbon electrode and AuNPs-horseradish peroxidase-streptavidin as nanotag. Analytica Chimica Acta, 1074, 80-88. https://doi.org/10.1016/j.aca.2019.05.012
Ye Y, et al. Electrochemical Detection of Salmonella Using an invA Genosensor On Polypyrrole-reduced Graphene Oxide Modified Glassy Carbon Electrode and AuNPs-horseradish Peroxidase-streptavidin as Nanotag. Anal Chim Acta. 2019 Oct 3;1074:80-88. PubMed PMID: 31159942.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemical detection of Salmonella using an invA genosensor on polypyrrole-reduced graphene oxide modified glassy carbon electrode and AuNPs-horseradish peroxidase-streptavidin as nanotag. AU - Ye,Yongkang, AU - Yan,Wuwen, AU - Liu,Yaqian, AU - He,Shudong, AU - Cao,Xiaodong, AU - Xu,Xuan, AU - Zheng,Haisong, AU - Gunasekaran,Sundaram, Y1 - 2019/05/08/ PY - 2019/04/15/received PY - 2019/05/03/revised PY - 2019/05/05/accepted PY - 2019/6/5/entrez PY - 2019/6/5/pubmed PY - 2019/9/14/medline KW - Electrochemical biosensor KW - Horseradish peroxidase-streptavidin biofunctionalized gold nanoparticles KW - Polypyrrole-reduced graphene oxide nanocomposite KW - Salmonella KW - invA gene SP - 80 EP - 88 JF - Analytica chimica acta JO - Anal Chim Acta VL - 1074 N2 - A rapid and sensitive electrochemical biosensor was constructed to detect Salmonella using invA gene biosensor. The biosensing was based on polyrrole-reduced graphene oxide (PPy-rGO) nanocomposite modified glassy carbon electrode (GCE) and signal amplification with horseradish peroxidase-streptavidin biofunctionalized gold nanoparticles (AuNPs-HRP-SA). PPy-rGO was prepared at 60 °C by chemical reduction of PPy-functionalized graphene oxide (PPy-GO) that was synthesized by in situ polymerization at room temperature. The detection signal was amplified via enzymatic reduction of H2O2 in the presence of hydroquinone (HQ) using AuNPs-HRP-SA as nanotag. Under optimal conditions, the differential pulse voltametric (DPV) signal from the biosensor was linearly related to the logarithm of target invA gene concentrations from 1.0 × 10-16 to 1.0 × 10-10 M, and the limit of detection (LOD) was 4.7 × 10-17 M. The biosensor can also detect Salmonella in the range of 9.6 to 9.6 × 104 CFU mL-1, with LOD of 8.07 CFU mL-1. The biosensor showed good regeneration ability, acceptable selectivity, repeatability and stability, which bode well as an alternative method for Salmonella screening. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/31159942/Electrochemical_detection_of_Salmonella_using_an_invA_genosensor_on_polypyrrole_reduced_graphene_oxide_modified_glassy_carbon_electrode_and_AuNPs_horseradish_peroxidase_streptavidin_as_nanotag_ DB - PRIME DP - Unbound Medicine ER -