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The application of Compont gel in chronic obstructive jaundice rats model.
Acta Cir Bras 2019; 34(5):e201900504AC

Abstract

PURPOSE

To establish a new rat model, the pathogenesis of which is closer to the clinical occurrence of chronic obstructive jaundice with liver fibrosis.

METHODS

90 SD rats were randomly divided into 3 groups. Group A common bile duct ligation, group B common bile duct injection compont and group C injection saline. The serum of three groups was extracted, and the liver function was detected by ELISA. HE staining, Masson staining and immunohistochemistry were used to detect liver pathology.

RESULTS

Group B showed a fluctuant development of jaundice, obstructive degree reached a peak at 2 weeks, and decreased from 3 weeks. HA, LA and PCIII were significantly higher than control group. 3 weeks after surgery, liver tissue fibrosis occurred in group B, and a wide range of fiber spacing was formed at 5 weeks. Immunohistochemistry showed that hepatic stellate cells were more active than the control group.

CONCLUSION

Intra-biliary injection of Compont gel is different from the classic obstructive jaundice animal model caused by classic bile duct ligation, which can provide an ideal rat model of chronic obstructive jaundice with liver fibrosis.

Authors+Show Affiliations

Guangxi Key Laboratory of Metabolic Diseases Research, Nephrology Department, Guilin NO. 924 Hospital, Guilin, Guangxi, China.Department of Hepatobiliary Surgery, First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi, China.Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei, China.Gulin Key Laboratory of Kidney Diseases Research, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin NO. 924 Hospital, Guilin, Guangxi, China.Department of General Surgery, The Affiliated Xingtai People's Hospital, Hebei Medical University, Xingtai, Hebei, China.Laboratory of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital, Guilin Medical University, Guilin, Guangxi, China.Department of Hepatobiliary Surgery, First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi, China.

Pub Type(s)

Evaluation Studies
Journal Article

Language

eng

PubMed ID

31166460

Citation

Luo, Wei-Wei, et al. "The Application of Compont Gel in Chronic Obstructive Jaundice Rats Model." Acta Cirurgica Brasileira, vol. 34, no. 5, 2019, pp. e201900504.
Luo WW, Zhou XL, Wang QQ, et al. The application of Compont gel in chronic obstructive jaundice rats model. Acta Cir Bras. 2019;34(5):e201900504.
Luo, W. W., Zhou, X. L., Wang, Q. Q., Shao, Y. J., Li, Z. M., Zhao, D. K., & Yu, S. P. (2019). The application of Compont gel in chronic obstructive jaundice rats model. Acta Cirurgica Brasileira, 34(5), pp. e201900504. doi:10.1590/s0102-865020190050000004.
Luo WW, et al. The Application of Compont Gel in Chronic Obstructive Jaundice Rats Model. Acta Cir Bras. 2019;34(5):e201900504. PubMed PMID: 31166460.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The application of Compont gel in chronic obstructive jaundice rats model. AU - Luo,Wei-Wei, AU - Zhou,Xue-Ling, AU - Wang,Qing-Qing, AU - Shao,Yan-Jing, AU - Li,Ze-Ming, AU - Zhao,Dong-Kang, AU - Yu,Shui-Ping, Y1 - 2019/06/03/ PY - 2019/01/12/received PY - 2019/04/09/accepted PY - 2019/6/6/entrez PY - 2019/6/6/pubmed PY - 2019/6/6/medline SP - e201900504 EP - e201900504 JF - Acta cirurgica brasileira JO - Acta Cir Bras VL - 34 IS - 5 N2 - PURPOSE: To establish a new rat model, the pathogenesis of which is closer to the clinical occurrence of chronic obstructive jaundice with liver fibrosis. METHODS: 90 SD rats were randomly divided into 3 groups. Group A common bile duct ligation, group B common bile duct injection compont and group C injection saline. The serum of three groups was extracted, and the liver function was detected by ELISA. HE staining, Masson staining and immunohistochemistry were used to detect liver pathology. RESULTS: Group B showed a fluctuant development of jaundice, obstructive degree reached a peak at 2 weeks, and decreased from 3 weeks. HA, LA and PCIII were significantly higher than control group. 3 weeks after surgery, liver tissue fibrosis occurred in group B, and a wide range of fiber spacing was formed at 5 weeks. Immunohistochemistry showed that hepatic stellate cells were more active than the control group. CONCLUSION: Intra-biliary injection of Compont gel is different from the classic obstructive jaundice animal model caused by classic bile duct ligation, which can provide an ideal rat model of chronic obstructive jaundice with liver fibrosis. SN - 1678-2674 UR - https://www.unboundmedicine.com/medline/citation/31166460/The_application_of_Compont_gel_in_chronic_obstructive_jaundice_rats_model L2 - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502019000500203&lng=en&nrm=iso&tlng=en DB - PRIME DP - Unbound Medicine ER -