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Comparison of volatile profiles and bioactive components of sun-dried Pu-erh tea leaves from ancient tea plants on Bulang Mountain measured by GC-MS and HPLC.
J Zhejiang Univ Sci B. 2019 Jul; 20(7):563-575.JZ

Abstract

To explore the volatile profiles and the contents of ten bioactive components (polyphenols and caffeine) of sun-dried Pu-erh tea leaves from ancient tea plants on Bulang Mountain, 17 samples of three tea varieties were analyzed by headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) and high-performance liquid chromatography (HPLC). A total of 75 volatile components were tentatively identified. Laomaner (LME), Laobanzhang (LBZ), and other teas on Bulang Mountain (BL) contained 70, 53, and 71 volatile compounds, respectively. Among the volatile compounds, alcohols (30.2%-45.8%), hydrocarbons (13.7%-17.5%), and ketones (12.4%-23.4%) were qualitatively the most dominant volatile compounds in the different tea varieties. The average content of polyphenol was highest in LME (102.1 mg/g), followed by BL (98.7 mg/g) and LBZ (88.0 mg/g), while caffeine showed the opposite trend, 27.3 mg/g in LME, 33.5 mg/g in BL, and 38.1 mg/g in LBZ. Principal component analysis applied to both the volatile compounds and ten bioactive components showed a poor separation of samples according to varieties, while partial least squares-discriminant analysis (PLS-DA) showed satisfactory discrimination. Thirty-four volatile components and five bioactive compounds were selected as major discriminators (variable importance in projection (VIP) >1) among the tea varieties. These results suggest that chromatographic data combined with multivariate analysis could provide a useful technique to characterize and distinguish the sun-dried Pu-erh tea leaves from ancient tea varieties on Bulang Mountain.

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Authors+Show Affiliations

College of Tea (Pu'er), West Yunnan University of Applied Sciences, Pu'er 665000, China. Pu'er Institute of Pu-erh Tea, Pu'er 665000, China.

College of Tea (Pu'er), West Yunnan University of Applied Sciences, Pu'er 665000, China. Pu'er Institute of Pu-erh Tea, Pu'er 665000, China.

College of Tea (Pu'er), West Yunnan University of Applied Sciences, Pu'er 665000, China. Pu'er Institute of Pu-erh Tea, Pu'er 665000, China.

College of Tea (Pu'er), West Yunnan University of Applied Sciences, Pu'er 665000, China. Pu'er Institute of Pu-erh Tea, Pu'er 665000, China.

College of Tea (Pu'er), West Yunnan University of Applied Sciences, Pu'er 665000, China. Pu'er Institute of Pu-erh Tea, Pu'er 665000, China.

College of Tea (Pu'er), West Yunnan University of Applied Sciences, Pu'er 665000, China. Pu'er Institute of Pu-erh Tea, Pu'er 665000, China.

College of Tea (Pu'er), West Yunnan University of Applied Sciences, Pu'er 665000, China. Pu'er Institute of Pu-erh Tea, Pu'er 665000, China.

MeSH

CaffeineCamellia sinensisChromatography, High Pressure LiquidDiscriminant AnalysisGas Chromatography-Mass SpectrometryLeast-Squares AnalysisMultivariate AnalysisPhytochemicalsPlant LeavesPolyphenolsPrincipal Component AnalysisSolid Phase MicroextractionTeaVolatile Organic Compounds

Pub Type(s)

Comparative Study

Journal Article

Language

eng

PubMed ID

31168970

Citation

Zhang, Wen-Jie, et al. "Comparison of Volatile Profiles and Bioactive Components of Sun-dried Pu-erh Tea Leaves From Ancient Tea Plants On Bulang Mountain Measured By GC-MS and HPLC." Journal of Zhejiang University. Science. B, vol. 20, no. 7, 2019, pp. 563-575.

Zhang WJ, Liu C, Yang RJ, et al. Comparison of volatile profiles and bioactive components of sun-dried Pu-erh tea leaves from ancient tea plants on Bulang Mountain measured by GC-MS and HPLC. J Zhejiang Univ Sci B. 2019;20(7):563-575.

Zhang, W. J., Liu, C., Yang, R. J., Zheng, T. T., Zhao, M. M., Ma, L., & Yan, L. (2019). Comparison of volatile profiles and bioactive components of sun-dried Pu-erh tea leaves from ancient tea plants on Bulang Mountain measured by GC-MS and HPLC. Journal of Zhejiang University. Science. B, 20(7), 563-575. https://doi.org/10.1631/jzus.B1800183

Zhang WJ, et al. Comparison of Volatile Profiles and Bioactive Components of Sun-dried Pu-erh Tea Leaves From Ancient Tea Plants On Bulang Mountain Measured By GC-MS and HPLC. J Zhejiang Univ Sci B. 2019;20(7):563-575. PubMed PMID: 31168970.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Comparison of volatile profiles and bioactive components of sun-dried Pu-erh tea leaves from ancient tea plants on Bulang Mountain measured by GC-MS and HPLC. AU - Zhang,Wen-Jie, AU - Liu,Cong, AU - Yang,Rui-Juan, AU - Zheng,Ting-Ting, AU - Zhao,Miao-Miao, AU - Ma,Li, AU - Yan,Liang, PY - 2019/6/7/entrez PY - 2019/6/7/pubmed PY - 2020/1/8/medline KW - Sun-dried Pu-erh tea; Ancient tea plant; Bulang Mountain; Volatile compound; Bioactive component SP - 563 EP - 575 JF - Journal of Zhejiang University. Science. B JO - J Zhejiang Univ Sci B VL - 20 IS - 7 N2 - To explore the volatile profiles and the contents of ten bioactive components (polyphenols and caffeine) of sun-dried Pu-erh tea leaves from ancient tea plants on Bulang Mountain, 17 samples of three tea varieties were analyzed by headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) and high-performance liquid chromatography (HPLC). A total of 75 volatile components were tentatively identified. Laomaner (LME), Laobanzhang (LBZ), and other teas on Bulang Mountain (BL) contained 70, 53, and 71 volatile compounds, respectively. Among the volatile compounds, alcohols (30.2%-45.8%), hydrocarbons (13.7%-17.5%), and ketones (12.4%-23.4%) were qualitatively the most dominant volatile compounds in the different tea varieties. The average content of polyphenol was highest in LME (102.1 mg/g), followed by BL (98.7 mg/g) and LBZ (88.0 mg/g), while caffeine showed the opposite trend, 27.3 mg/g in LME, 33.5 mg/g in BL, and 38.1 mg/g in LBZ. Principal component analysis applied to both the volatile compounds and ten bioactive components showed a poor separation of samples according to varieties, while partial least squares-discriminant analysis (PLS-DA) showed satisfactory discrimination. Thirty-four volatile components and five bioactive compounds were selected as major discriminators (variable importance in projection (VIP) >1) among the tea varieties. These results suggest that chromatographic data combined with multivariate analysis could provide a useful technique to characterize and distinguish the sun-dried Pu-erh tea leaves from ancient tea varieties on Bulang Mountain. SN - 1862-1783 UR - https://www.unboundmedicine.com/medline/citation/31168970/Comparison_of_volatile_profiles_and_bioactive_components_of_sun_dried_Pu_erh_tea_leaves_from_ancient_tea_plants_on_Bulang_Mountain_measured_by_GC_MS_and_HPLC_ DB - PRIME DP - Unbound Medicine ER -