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Lack of association between methylenetetrahydrofolate reductase C677T polymorphism, HPV infection and cervical intraepithelial neoplasia in Brazilian women.
BMC Med Genet 2019; 20(1):100BM

Abstract

BACKGROUND

Cervical cancer has high prevalence and mortality rates in worldwide female population. Persistent infection by high-risk Human Papillomavirus (hr-HPV) is the main cause of this cancer. However, many environmental, genetical, and epigenetical cofactors can modulate viral infection and cervical carcinogenesis. Methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism is a genetic factor that has been associated with many pathologies, including cancer. Nevertheless, studies with cervical cancer presented controversial results, and varied according to ethnicity. Thus, the aim of this study was to determine association between MTHFR C677T polymorphism, Human Papillomavirus (HPV) infection and cervical cancer.

METHODS

A case-control study was performed with 150 histological cervical samples. Case group were divided in Cervical Intraepithelial Neoplasia (CIN) grade I (n = 30), CIN II (n = 30), CIN III (n = 30), and Squamous Cervical Carcinoma (SCC) (n = 30). Control group was composed by 30 samples without lesion, presenting cervicitis. HPV detection was performed by conventional Polymerase Chain Reaction (PCR) with SPF primers set, and by real-time PCR specific for HPV 16 and hr-HPV. MTHFR C677T polymorphism was analyzed by PCR followed by Restriction Fragment Length Polymorphism (RFLP).

RESULTS

Frequency of MTHFR CC genotype was 72.7% (n = 109), CT 23.3% (n = 35) and TT 4.0% (n = 6). Polymorphic T allele frequency was 15.7%. No statistically significant association was observed between MTHFR C677T polymorphism and presence of pre-neoplastic or neoplastic cervical lesions. Similar frequencies of T allele was observed in control (23.3%) and cases (13.3%) groups (p = 0.174). In addition, there was no statistically significant association between MTHFR C677T polymorphism and viral infection, even considering hr-HPV or HPV 16 positivity.

CONCLUSION

MTHFR C677T polymorphism was not associated with cervical cancer and HPV infection.

Authors+Show Affiliations

Programa de Pós-Graduação em Ciências Farmacêuticas, Departamento de Análises Clínicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, 35400-000, Brazil. nayarants@gmail.com.Departamento de Análises Clínicas e Toxicológicas, Universidade Federal de Minas Gerais, President Antônio Carlos Avenue, 6627, Belo Horizonte, Minas Gerais, 31270-901, Brazil.Laboratório Tafuri, São Paulo Street, 893, Belo Horizonte, Minas Gerais, 30170-131, Brazil.Programa de Pós-Graduação em Ciências Farmacêuticas, Departamento de Análises Clínicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, 35400-000, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31170928

Citation

Silva, Nayara Nascimento Toledo, et al. "Lack of Association Between Methylenetetrahydrofolate Reductase C677T Polymorphism, HPV Infection and Cervical Intraepithelial Neoplasia in Brazilian Women." BMC Medical Genetics, vol. 20, no. 1, 2019, p. 100.
Silva NNT, Sabino AP, Tafuri A, et al. Lack of association between methylenetetrahydrofolate reductase C677T polymorphism, HPV infection and cervical intraepithelial neoplasia in Brazilian women. BMC Med Genet. 2019;20(1):100.
Silva, N. N. T., Sabino, A. P., Tafuri, A., & Lima, A. A. (2019). Lack of association between methylenetetrahydrofolate reductase C677T polymorphism, HPV infection and cervical intraepithelial neoplasia in Brazilian women. BMC Medical Genetics, 20(1), p. 100. doi:10.1186/s12881-019-0831-x.
Silva NNT, et al. Lack of Association Between Methylenetetrahydrofolate Reductase C677T Polymorphism, HPV Infection and Cervical Intraepithelial Neoplasia in Brazilian Women. BMC Med Genet. 2019 06 6;20(1):100. PubMed PMID: 31170928.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lack of association between methylenetetrahydrofolate reductase C677T polymorphism, HPV infection and cervical intraepithelial neoplasia in Brazilian women. AU - Silva,Nayara Nascimento Toledo, AU - Sabino,Adriano de Paula, AU - Tafuri,Alexandre, AU - Lima,Angélica Alves, Y1 - 2019/06/06/ PY - 2018/08/31/received PY - 2019/05/21/accepted PY - 2019/6/8/entrez PY - 2019/6/7/pubmed PY - 2019/10/1/medline KW - Cervical Cancer KW - Folate metabolism KW - HPV KW - MTHFR C677T polymorphism KW - SNP SP - 100 EP - 100 JF - BMC medical genetics JO - BMC Med. Genet. VL - 20 IS - 1 N2 - BACKGROUND: Cervical cancer has high prevalence and mortality rates in worldwide female population. Persistent infection by high-risk Human Papillomavirus (hr-HPV) is the main cause of this cancer. However, many environmental, genetical, and epigenetical cofactors can modulate viral infection and cervical carcinogenesis. Methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism is a genetic factor that has been associated with many pathologies, including cancer. Nevertheless, studies with cervical cancer presented controversial results, and varied according to ethnicity. Thus, the aim of this study was to determine association between MTHFR C677T polymorphism, Human Papillomavirus (HPV) infection and cervical cancer. METHODS: A case-control study was performed with 150 histological cervical samples. Case group were divided in Cervical Intraepithelial Neoplasia (CIN) grade I (n = 30), CIN II (n = 30), CIN III (n = 30), and Squamous Cervical Carcinoma (SCC) (n = 30). Control group was composed by 30 samples without lesion, presenting cervicitis. HPV detection was performed by conventional Polymerase Chain Reaction (PCR) with SPF primers set, and by real-time PCR specific for HPV 16 and hr-HPV. MTHFR C677T polymorphism was analyzed by PCR followed by Restriction Fragment Length Polymorphism (RFLP). RESULTS: Frequency of MTHFR CC genotype was 72.7% (n = 109), CT 23.3% (n = 35) and TT 4.0% (n = 6). Polymorphic T allele frequency was 15.7%. No statistically significant association was observed between MTHFR C677T polymorphism and presence of pre-neoplastic or neoplastic cervical lesions. Similar frequencies of T allele was observed in control (23.3%) and cases (13.3%) groups (p = 0.174). In addition, there was no statistically significant association between MTHFR C677T polymorphism and viral infection, even considering hr-HPV or HPV 16 positivity. CONCLUSION: MTHFR C677T polymorphism was not associated with cervical cancer and HPV infection. SN - 1471-2350 UR - https://www.unboundmedicine.com/medline/citation/31170928/Lack_of_association_between_methylenetetrahydrofolate_reductase_C677T_polymorphism_HPV_infection_and_cervical_intraepithelial_neoplasia_in_Brazilian_women_ L2 - https://bmcmedgenet.biomedcentral.com/articles/10.1186/s12881-019-0831-x DB - PRIME DP - Unbound Medicine ER -