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Protective effects of hydrogen inhalation during the warm ischemia phase against lung ischemia-reperfusion injury in rat donors after cardiac death.
Microvasc Res. 2019 09; 125:103885.MR

Abstract

BACKGROUND

Successful amelioration of long-term warm ischemia lung injury in donors after cardiac death (DCDs) can remarkably improve outcomes. Hydrogen gas provides potent anti-inflammatory and antioxidant effects against ischemia-reperfusion injury (IRI). This study observed the effects of hydrogen inhalation on lung grafts during the warm ischemia phase in cardiac death donors.

METHODS

After cardiac death, rat donor lungs (n = 8) underwent mechanical ventilation with 40% oxygen plus 60% nitrogen (control group) or 3% hydrogen and 40% oxygen plus 57% nitrogen (hydrogen group) for 2 h during the warm ischemia phase in situ. Then, lung transplantation was performed after 2 h of cold storage and 3 h of recipient reperfusion prior to lung graft assessment. Rats that underwent left thoracotomy without transplantation served as the sham group (n = 8). The results of static compliance and arterial blood gas analysis were assessed in the recipients. The wet-to-dry weight ratio (W/D), inflammation, oxidative stress, cell apoptosis and histologic changes were evaluated after 3 h of reperfusion. Nuclear factor kappa B (NF-κB) protein expression in the graft was analyzed by Western blotting.

RESULTS

Compared with the sham group, lung function, W/D, inflammatory reaction, oxidative stress and histological changes were decreased in both transplant groups (control and hydrogen groups). However, compared with the control group, exposure to 3% hydrogen significantly improved lung graft static compliance and oxygenation and remarkably decreased the wet-to-dry weight ratio, inflammatory reactions, and lipid peroxidation. Furthermore, hydrogen improved the lung graft histological changes, decreased the lung injury score and apoptotic index and reduced NF-κB nuclear accumulation in the lung grafts.

CONCLUSION

Lung inhalation with 3% hydrogen during the warm ischemia phase attenuated lung graft IRI via NF-κB-dependent anti-inflammatory and antioxidative effects in rat donors after cardiac death.

Authors+Show Affiliations

Department of Anesthesiology, The Second Affiliated Hospital of Harbin Medical University, The Hei Long jiang Province Key Lab of Research on Anesthesiology and Critical Care Medicine, Harbin, China.Department of Anesthesiology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, China.Department of Anesthesiology, The Second Affiliated Hospital of Harbin Medical University, The Hei Long jiang Province Key Lab of Research on Anesthesiology and Critical Care Medicine, Harbin, China.The Affiliated Hospital of Qingdao University, Qingdao, China.Department of Anesthesiology, The Third Affiliated Hospital of Harbin Medical University, Harbin, China.Department of Anesthesiology, The Second Affiliated Hospital of Harbin Medical University, The Hei Long jiang Province Key Lab of Research on Anesthesiology and Critical Care Medicine, Harbin, China. Electronic address: wenzhili9@126.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31175855

Citation

Zhang, Jiahang, et al. "Protective Effects of Hydrogen Inhalation During the Warm Ischemia Phase Against Lung Ischemia-reperfusion Injury in Rat Donors After Cardiac Death." Microvascular Research, vol. 125, 2019, p. 103885.
Zhang J, Zhou H, Liu J, et al. Protective effects of hydrogen inhalation during the warm ischemia phase against lung ischemia-reperfusion injury in rat donors after cardiac death. Microvasc Res. 2019;125:103885.
Zhang, J., Zhou, H., Liu, J., Meng, C., Deng, L., & Li, W. (2019). Protective effects of hydrogen inhalation during the warm ischemia phase against lung ischemia-reperfusion injury in rat donors after cardiac death. Microvascular Research, 125, 103885. https://doi.org/10.1016/j.mvr.2019.103885
Zhang J, et al. Protective Effects of Hydrogen Inhalation During the Warm Ischemia Phase Against Lung Ischemia-reperfusion Injury in Rat Donors After Cardiac Death. Microvasc Res. 2019;125:103885. PubMed PMID: 31175855.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of hydrogen inhalation during the warm ischemia phase against lung ischemia-reperfusion injury in rat donors after cardiac death. AU - Zhang,Jiahang, AU - Zhou,Huacheng, AU - Liu,Jinfeng, AU - Meng,Chao, AU - Deng,Lin, AU - Li,Wenzhi, Y1 - 2019/06/05/ PY - 2018/11/10/received PY - 2019/05/09/revised PY - 2019/06/03/accepted PY - 2019/6/9/pubmed PY - 2020/5/6/medline PY - 2019/6/9/entrez KW - Hydrogen KW - Ischemia-reperfusion injury KW - Warm ischemia phase SP - 103885 EP - 103885 JF - Microvascular research JO - Microvasc Res VL - 125 N2 - BACKGROUND: Successful amelioration of long-term warm ischemia lung injury in donors after cardiac death (DCDs) can remarkably improve outcomes. Hydrogen gas provides potent anti-inflammatory and antioxidant effects against ischemia-reperfusion injury (IRI). This study observed the effects of hydrogen inhalation on lung grafts during the warm ischemia phase in cardiac death donors. METHODS: After cardiac death, rat donor lungs (n = 8) underwent mechanical ventilation with 40% oxygen plus 60% nitrogen (control group) or 3% hydrogen and 40% oxygen plus 57% nitrogen (hydrogen group) for 2 h during the warm ischemia phase in situ. Then, lung transplantation was performed after 2 h of cold storage and 3 h of recipient reperfusion prior to lung graft assessment. Rats that underwent left thoracotomy without transplantation served as the sham group (n = 8). The results of static compliance and arterial blood gas analysis were assessed in the recipients. The wet-to-dry weight ratio (W/D), inflammation, oxidative stress, cell apoptosis and histologic changes were evaluated after 3 h of reperfusion. Nuclear factor kappa B (NF-κB) protein expression in the graft was analyzed by Western blotting. RESULTS: Compared with the sham group, lung function, W/D, inflammatory reaction, oxidative stress and histological changes were decreased in both transplant groups (control and hydrogen groups). However, compared with the control group, exposure to 3% hydrogen significantly improved lung graft static compliance and oxygenation and remarkably decreased the wet-to-dry weight ratio, inflammatory reactions, and lipid peroxidation. Furthermore, hydrogen improved the lung graft histological changes, decreased the lung injury score and apoptotic index and reduced NF-κB nuclear accumulation in the lung grafts. CONCLUSION: Lung inhalation with 3% hydrogen during the warm ischemia phase attenuated lung graft IRI via NF-κB-dependent anti-inflammatory and antioxidative effects in rat donors after cardiac death. SN - 1095-9319 UR - https://www.unboundmedicine.com/medline/citation/31175855/Protective_effects_of_hydrogen_inhalation_during_the_warm_ischemia_phase_against_lung_ischemia_reperfusion_injury_in_rat_donors_after_cardiac_death_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0026-2862(18)30278-4 DB - PRIME DP - Unbound Medicine ER -