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Stem cell passage affects directional migration of stem cells in electrotaxis.
Stem Cell Res 2019; 38:101475SC

Abstract

Stem cells can differentiate into various body tissues and organs and thus are considered as promising tools for cell therapy and tissue engineering. Early passage stem cells have high differentiation ability compared to late passage stem cells. Thus, it is important to use early passage stem cells in cell therapy. Here, we investigated whether cell migration could be used to compare young and senescent cells. We used 'electrotaxis' where cells under electric treatment move towards the anode or cathode. Without an electric stimulus, stem cells moved randomly. However, under a direct electric current, the cells moved with directionality. Under stimulation with a direct electric current, early passage stem cells moved towards the anode; when the cells became senescent with increasing passages, the percentage of cells migrating to the anode decreased. These results suggest that the behavior of stem cells under the influence of a direct electric current is also related to their passage number. Therefore, electrotaxis migration analysis can be used to distinguish between young cell and senescent cells.

Authors+Show Affiliations

Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea.Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea.Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea.Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea.Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea; Department of Medical Device Industry, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea.Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea.Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea; Department of Medical Device Industry, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea. Electronic address: parkjc@yuhs.ac.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31176110

Citation

Hong, Seung Hee, et al. "Stem Cell Passage Affects Directional Migration of Stem Cells in Electrotaxis." Stem Cell Research, vol. 38, 2019, p. 101475.
Hong SH, Lee MH, Koo MA, et al. Stem cell passage affects directional migration of stem cells in electrotaxis. Stem Cell Res. 2019;38:101475.
Hong, S. H., Lee, M. H., Koo, M. A., Seon, G. M., Park, Y. J., Kim, D., & Park, J. C. (2019). Stem cell passage affects directional migration of stem cells in electrotaxis. Stem Cell Research, 38, p. 101475. doi:10.1016/j.scr.2019.101475.
Hong SH, et al. Stem Cell Passage Affects Directional Migration of Stem Cells in Electrotaxis. Stem Cell Res. 2019;38:101475. PubMed PMID: 31176110.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stem cell passage affects directional migration of stem cells in electrotaxis. AU - Hong,Seung Hee, AU - Lee,Mi Hee, AU - Koo,Min-Ah, AU - Seon,Gyeung Mi, AU - Park,Ye Jin, AU - Kim,Dohyun, AU - Park,Jong-Chul, Y1 - 2019/05/30/ PY - 2019/01/29/received PY - 2019/05/20/revised PY - 2019/05/28/accepted PY - 2019/6/9/pubmed PY - 2019/6/9/medline PY - 2019/6/9/entrez KW - Directional migration KW - Electrotaxis KW - Senescence KW - Stem cell SP - 101475 EP - 101475 JF - Stem cell research JO - Stem Cell Res VL - 38 N2 - Stem cells can differentiate into various body tissues and organs and thus are considered as promising tools for cell therapy and tissue engineering. Early passage stem cells have high differentiation ability compared to late passage stem cells. Thus, it is important to use early passage stem cells in cell therapy. Here, we investigated whether cell migration could be used to compare young and senescent cells. We used 'electrotaxis' where cells under electric treatment move towards the anode or cathode. Without an electric stimulus, stem cells moved randomly. However, under a direct electric current, the cells moved with directionality. Under stimulation with a direct electric current, early passage stem cells moved towards the anode; when the cells became senescent with increasing passages, the percentage of cells migrating to the anode decreased. These results suggest that the behavior of stem cells under the influence of a direct electric current is also related to their passage number. Therefore, electrotaxis migration analysis can be used to distinguish between young cell and senescent cells. SN - 1876-7753 UR - https://www.unboundmedicine.com/medline/citation/31176110/Stem_cell_passage_affects_directional_migration_of_stem_cells_in_electrotaxis L2 - https://linkinghub.elsevier.com/retrieve/pii/S1873-5061(19)30105-9 DB - PRIME DP - Unbound Medicine ER -