Tags

Type your tag names separated by a space and hit enter

Metal bioaccumulation, oxidative stress and antioxidant responses in oysters Crassostrea gasar transplanted to an estuary in southern Brazil.
Sci Total Environ. 2019 Oct 01; 685:332-344.ST

Abstract

The present study assessed the spatial and temporal variations on metal bioaccumulation and biochemical biomarker responses in oysters Crassostrea gasar transplanted to two different sites (S1 and S2) at the Laguna Estuarine System (LES), southern Brazil, over a 45-days period. A multi-biomarker approach was used, including the evaluation of lipid peroxidation (MDA) levels, and antioxidant defense enzymes (CAT, GPx, GR and G6PDH) and phase II biotransformation enzyme (GST) in the gills and digestive gland of oysters in combination with the quantification of Al, Cd, Cu, Pb, Fe, Ni and Zn in both tissues. The exposed oysters bioaccumulated metals, especially Al, Cd and Zn in gills and digestive gland, with most prominent biomarker responses in the gills. Results showed that GPx, GR and G6PDH enzymes offered an increased and coordinated response possibly against metal (Zn, Ni, Cd and Cu) contamination in gills. GST was inversely correlated to Cd levels, being its activity significantly lowered over the 45-d exposure periods at S2. On contrary, in digestive gland GST was slightly positively correlated to Cd, revealing a compensatory mechanism between tissues to protect oysters' cells against oxidative damages, since MDA levels also decreased. CAT also appeared to be involved in the cellular protection against oxidative stress, being increased in gills. However, CAT was negatively correlated to Al levels, which might suggest a possible inhibitory effect of this metal in the gills of C. gasar. Differences between tissues were evident by the Integrative Biomarker Responses version 2 (IBRv2) indexes, which showed different pattern between tissues when studying the sites and exposure periods separately. This study provided evidence for the effectiveness of using a multi-biomarker approach in oyster C. gasar to monitor estuarine metal pollution.

Authors+Show Affiliations

Fishery Engineering Department, Santa Catarina State University, Laguna 88790-000, Brazil.Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Federal University of Santa Catarina, Florianópolis 88034-257, Brazil.Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Federal University of Santa Catarina, Florianópolis 88034-257, Brazil.Fishery Engineering Department, Santa Catarina State University, Laguna 88790-000, Brazil.Aquaculture Pathology Research Center, Federal University of Santa Catarina, Florianópolis 88034-257, Brazil.Department of Natural Sciences, Regional University of Blumenau, Blumenau 89012-170, Brazil.Department of Natural Sciences, Regional University of Blumenau, Blumenau 89012-170, Brazil.Department of Natural Sciences, Regional University of Blumenau, Blumenau 89012-170, Brazil.Oceanography and Limnology Department, Federal University of Maranhão, São Luís 65080-805, Brazil.Oceanography and Limnology Department, Federal University of Maranhão, São Luís 65080-805, Brazil.Animal and Food Production Department, Santa Catarina State University, Lages 88520-000, Brazil.Animal and Food Production Department, Santa Catarina State University, Lages 88520-000, Brazil.Laboratory of Marine Mollusks, Federal University of Santa Catarina, Florianópolis 88034-257, Brazil.Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Federal University of Santa Catarina, Florianópolis 88034-257, Brazil.Department of Scientific and Technological Education, Santa Catarina State University, Florianópolis 88035-001, Brazil. Electronic address: karim.luchmann@udesc.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31176220

Citation

Ferreira, Clarissa P., et al. "Metal Bioaccumulation, Oxidative Stress and Antioxidant Responses in Oysters Crassostrea Gasar Transplanted to an Estuary in Southern Brazil." The Science of the Total Environment, vol. 685, 2019, pp. 332-344.
Ferreira CP, Lima D, Paiva R, et al. Metal bioaccumulation, oxidative stress and antioxidant responses in oysters Crassostrea gasar transplanted to an estuary in southern Brazil. Sci Total Environ. 2019;685:332-344.
Ferreira, C. P., Lima, D., Paiva, R., Vilke, J. M., Mattos, J. J., Almeida, E. A., Grott, S. C., Alves, T. C., Corrêa, J. N., Jorge, M. B., Uczay, M., Vogel, C. I. G., Gomes, C. H. A. M., Bainy, A. C. D., & Lüchmann, K. H. (2019). Metal bioaccumulation, oxidative stress and antioxidant responses in oysters Crassostrea gasar transplanted to an estuary in southern Brazil. The Science of the Total Environment, 685, 332-344. https://doi.org/10.1016/j.scitotenv.2019.05.384
Ferreira CP, et al. Metal Bioaccumulation, Oxidative Stress and Antioxidant Responses in Oysters Crassostrea Gasar Transplanted to an Estuary in Southern Brazil. Sci Total Environ. 2019 Oct 1;685:332-344. PubMed PMID: 31176220.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metal bioaccumulation, oxidative stress and antioxidant responses in oysters Crassostrea gasar transplanted to an estuary in southern Brazil. AU - Ferreira,Clarissa P, AU - Lima,Daína, AU - Paiva,Raphaella, AU - Vilke,Juliano M, AU - Mattos,Jacó J, AU - Almeida,Eduardo A, AU - Grott,Suelen C, AU - Alves,Thiago C, AU - Corrêa,Jacyara N, AU - Jorge,Marianna B, AU - Uczay,Mariana, AU - Vogel,Carla I G, AU - Gomes,Carlos H A M, AU - Bainy,Afonso C D, AU - Lüchmann,Karim H, Y1 - 2019/05/28/ PY - 2018/12/22/received PY - 2019/05/07/revised PY - 2019/05/24/accepted PY - 2019/6/9/pubmed PY - 2019/9/13/medline PY - 2019/6/9/entrez KW - Biochemical biomarkers KW - Estuarine pollution KW - Metal KW - Oysters SP - 332 EP - 344 JF - The Science of the total environment JO - Sci Total Environ VL - 685 N2 - The present study assessed the spatial and temporal variations on metal bioaccumulation and biochemical biomarker responses in oysters Crassostrea gasar transplanted to two different sites (S1 and S2) at the Laguna Estuarine System (LES), southern Brazil, over a 45-days period. A multi-biomarker approach was used, including the evaluation of lipid peroxidation (MDA) levels, and antioxidant defense enzymes (CAT, GPx, GR and G6PDH) and phase II biotransformation enzyme (GST) in the gills and digestive gland of oysters in combination with the quantification of Al, Cd, Cu, Pb, Fe, Ni and Zn in both tissues. The exposed oysters bioaccumulated metals, especially Al, Cd and Zn in gills and digestive gland, with most prominent biomarker responses in the gills. Results showed that GPx, GR and G6PDH enzymes offered an increased and coordinated response possibly against metal (Zn, Ni, Cd and Cu) contamination in gills. GST was inversely correlated to Cd levels, being its activity significantly lowered over the 45-d exposure periods at S2. On contrary, in digestive gland GST was slightly positively correlated to Cd, revealing a compensatory mechanism between tissues to protect oysters' cells against oxidative damages, since MDA levels also decreased. CAT also appeared to be involved in the cellular protection against oxidative stress, being increased in gills. However, CAT was negatively correlated to Al levels, which might suggest a possible inhibitory effect of this metal in the gills of C. gasar. Differences between tissues were evident by the Integrative Biomarker Responses version 2 (IBRv2) indexes, which showed different pattern between tissues when studying the sites and exposure periods separately. This study provided evidence for the effectiveness of using a multi-biomarker approach in oyster C. gasar to monitor estuarine metal pollution. SN - 1879-1026 UR - https://www.unboundmedicine.com/medline/citation/31176220/Metal_bioaccumulation_oxidative_stress_and_antioxidant_responses_in_oysters_Crassostrea_gasar_transplanted_to_an_estuary_in_southern_Brazil_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-9697(19)32437-4 DB - PRIME DP - Unbound Medicine ER -