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Diversity of staphylococcal species in food producing animals in Spain, with detection of PVL-positive MRSA ST8 (USA300).
Vet Microbiol. 2019 Jun; 233:5-10.VM

Abstract

This work aimed to determine the prevalence, diversity, antibiotic-resistance phenotype/genotype and virulence factors in staphylococci of farm-animals. Nasal samples of 117 farm-animals (calve: 72; lamb: 37; goat: 8) were collected from one slaughterhouse in La Rioja/Spain and cultured for staphylococci and methicillin-resistant Staphylococcus (MRS) recovery. Identification was performed by MALDI-TOF. Antimicrobial resistance phenotype/genotype was determined by susceptibility testing and specific PCRs. Molecular typing (spa-typing, multilocus-sequence-typing, agr-typing, SCCmec), and detection of 12 virulence genes and human Immune-evasive-cluster (IEC) genes were performed by PCR/sequencing in S. aureus. Two marker genes of arginine catabolic mobile element (ACME) were determined by PCR (USA300-MRSA detection). Staphylococci were identified in 50%, 54% and 21% of goat, lamb and calve samples, respectively. Among the 13 S. aureus isolates recovered, 11 were susceptible to all antimicrobials tested, and two were multidrug-resistant-MRSA [beta-lactams (blaZ, mecA), macrolides [(msr(A)/msr(B)] and fluoroquinolones]. The MSSA harboured either tst or enterotoxin genes, while the MRSA harboured the lukF/lukS-PV genes. Five sequence-types were detected. The two MRSA strains (from lamb and goat) were typed as t5173/ST8/agr-I/SCCmec-IVa/ACME-positive, corresponding to USA300 clone, and were IEC-B-positive. Among the 47 coagulase-negative staphylococci (CoNS), six species were identified, predominating S. simulans (n = 25) and S. sciuri (n = 11). Fifty-three percent of CoNS showed resistance to at least one antimicrobial agent (six multidrug-resistant strains), and the following resistance phenotypes/genotypes were detected: streptomycin [27.6%; ant(6)-Ia, str], tetracycline [23.4%; tet(M), tet(L), tet(K)], clindamycin [19.1%; lnu(A), vgaA], erythromycin [10.6%; erm(C), msr(A)/msr(B)], chloramphenicol (8.5%; fexA), tobramycin (6.4%), penicillin-cefoxitin (4.3%; blaZ, mecA), and SXT (2.1%). The detection of the MRSA-USA300 lineage in food animals is worrisome and should be further monitored.

Authors+Show Affiliations

Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.Laboratory of Food Microbiology, Institute of Food, Nutrition and Health, ETH Zurich, Zurich, Switzerland; Área de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR), Logroño, Spain.Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain. Electronic address: carmen.torres@unirioja.es.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31176412

Citation

Mama, Olouwafemi Mistourath, et al. "Diversity of Staphylococcal Species in Food Producing Animals in Spain, With Detection of PVL-positive MRSA ST8 (USA300)." Veterinary Microbiology, vol. 233, 2019, pp. 5-10.
Mama OM, Gómez-Sanz E, Ruiz-Ripa L, et al. Diversity of staphylococcal species in food producing animals in Spain, with detection of PVL-positive MRSA ST8 (USA300). Vet Microbiol. 2019;233:5-10.
Mama, O. M., Gómez-Sanz, E., Ruiz-Ripa, L., Gómez, P., & Torres, C. (2019). Diversity of staphylococcal species in food producing animals in Spain, with detection of PVL-positive MRSA ST8 (USA300). Veterinary Microbiology, 233, 5-10. https://doi.org/10.1016/j.vetmic.2019.04.013
Mama OM, et al. Diversity of Staphylococcal Species in Food Producing Animals in Spain, With Detection of PVL-positive MRSA ST8 (USA300). Vet Microbiol. 2019;233:5-10. PubMed PMID: 31176412.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diversity of staphylococcal species in food producing animals in Spain, with detection of PVL-positive MRSA ST8 (USA300). AU - Mama,Olouwafemi Mistourath, AU - Gómez-Sanz,Elena, AU - Ruiz-Ripa,Laura, AU - Gómez,Paula, AU - Torres,Carmen, Y1 - 2019/04/12/ PY - 2019/01/30/received PY - 2019/04/09/revised PY - 2019/04/10/accepted PY - 2019/6/10/entrez PY - 2019/6/10/pubmed PY - 2019/6/27/medline KW - Food producing animals KW - IEC KW - MRSA USA300 KW - PVL KW - Staphylococcus spp KW - Virulence genes SP - 5 EP - 10 JF - Veterinary microbiology JO - Vet Microbiol VL - 233 N2 - This work aimed to determine the prevalence, diversity, antibiotic-resistance phenotype/genotype and virulence factors in staphylococci of farm-animals. Nasal samples of 117 farm-animals (calve: 72; lamb: 37; goat: 8) were collected from one slaughterhouse in La Rioja/Spain and cultured for staphylococci and methicillin-resistant Staphylococcus (MRS) recovery. Identification was performed by MALDI-TOF. Antimicrobial resistance phenotype/genotype was determined by susceptibility testing and specific PCRs. Molecular typing (spa-typing, multilocus-sequence-typing, agr-typing, SCCmec), and detection of 12 virulence genes and human Immune-evasive-cluster (IEC) genes were performed by PCR/sequencing in S. aureus. Two marker genes of arginine catabolic mobile element (ACME) were determined by PCR (USA300-MRSA detection). Staphylococci were identified in 50%, 54% and 21% of goat, lamb and calve samples, respectively. Among the 13 S. aureus isolates recovered, 11 were susceptible to all antimicrobials tested, and two were multidrug-resistant-MRSA [beta-lactams (blaZ, mecA), macrolides [(msr(A)/msr(B)] and fluoroquinolones]. The MSSA harboured either tst or enterotoxin genes, while the MRSA harboured the lukF/lukS-PV genes. Five sequence-types were detected. The two MRSA strains (from lamb and goat) were typed as t5173/ST8/agr-I/SCCmec-IVa/ACME-positive, corresponding to USA300 clone, and were IEC-B-positive. Among the 47 coagulase-negative staphylococci (CoNS), six species were identified, predominating S. simulans (n = 25) and S. sciuri (n = 11). Fifty-three percent of CoNS showed resistance to at least one antimicrobial agent (six multidrug-resistant strains), and the following resistance phenotypes/genotypes were detected: streptomycin [27.6%; ant(6)-Ia, str], tetracycline [23.4%; tet(M), tet(L), tet(K)], clindamycin [19.1%; lnu(A), vgaA], erythromycin [10.6%; erm(C), msr(A)/msr(B)], chloramphenicol (8.5%; fexA), tobramycin (6.4%), penicillin-cefoxitin (4.3%; blaZ, mecA), and SXT (2.1%). The detection of the MRSA-USA300 lineage in food animals is worrisome and should be further monitored. SN - 1873-2542 UR - https://www.unboundmedicine.com/medline/citation/31176412/Diversity_of_staphylococcal_species_in_food_producing_animals_in_Spain_with_detection_of_PVL_positive_MRSA_ST8__USA300__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(19)30153-1 DB - PRIME DP - Unbound Medicine ER -