Tags

Type your tag names separated by a space and hit enter

Fifteen Marseilleviruses Newly Isolated From Three Water Samples in Japan Reveal Local Diversity of Marseilleviridae.
Front Microbiol. 2019; 10:1152.FM

Abstract

The family Marseilleviridae, defined as a group of icosahedral double-stranded DNA viruses with particle size of approximately 250 nm and genome size of 350-380 kbp, belongs to the nucleo-cytoplasmic family of large DNA viruses. The family Marseilleviridae is currently classified into lineages A-E. In this study, we isolated 12 or 15 new members of the family Marseilleviridae from three sampling locations in Japan. Molecular phylogenetic analysis of the MCP genes showed that the new viruses could be further classified into three groups, hokutoviruses, kashiwazakiviruses, and kyotoviruses. Hokutoviruses were closely related to lineage B, kyotoviruses were related to lineage A, and kashiwazakiviruses were also classified into lineage B but a new putative subgroup of lineage B, revealing the diversity of this lineage. Interestingly, more than two viruses with slightly different MCP genes were isolated from a single water sample from a single location, i.e., two hokutoviruses and one kashiwazakivirus were isolated from a small reservoir, five kashiwazakiviruses from the mouth of a river, and five kyotoviruses from fresh water of a river, suggesting that several milliliters of water samples contain several types of giant viruses. Amoeba cells infected with hokutoviruses or kashiwazakiviruses exhibited a "bunch" formation consisting of normal and infected cells similarly to a tupanvirus, whereas cells infected with kyotoviruses or tokyovirus did not. These results suggest the previously unrecognized local diversity of the family Marseilleviridae in aquatic environments.

Authors+Show Affiliations

Laboratory of Biology, Graduate School of Mathematics and Science Education, Tokyo University of Science, Tokyo, Japan.Laboratory of Biology, Department of Liberal Arts, Faculty of Science, Tokyo University of Science, Tokyo, Japan.Laboratory of Biology, Department of Liberal Arts, Faculty of Science, Tokyo University of Science, Tokyo, Japan.Laboratory of Biology, Department of Liberal Arts, Faculty of Science, Tokyo University of Science, Tokyo, Japan.National Institute for Physiological Sciences, Okazaki, Japan.Institute for Chemical Research, Kyoto University, Gokasho, Japan.Laboratory of Biology, Graduate School of Mathematics and Science Education, Tokyo University of Science, Tokyo, Japan. Laboratory of Biology, Department of Liberal Arts, Faculty of Science, Tokyo University of Science, Tokyo, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31178850

Citation

Aoki, Keita, et al. "Fifteen Marseilleviruses Newly Isolated From Three Water Samples in Japan Reveal Local Diversity of Marseilleviridae." Frontiers in Microbiology, vol. 10, 2019, p. 1152.
Aoki K, Hagiwara R, Akashi M, et al. Fifteen Marseilleviruses Newly Isolated From Three Water Samples in Japan Reveal Local Diversity of Marseilleviridae. Front Microbiol. 2019;10:1152.
Aoki, K., Hagiwara, R., Akashi, M., Sasaki, K., Murata, K., Ogata, H., & Takemura, M. (2019). Fifteen Marseilleviruses Newly Isolated From Three Water Samples in Japan Reveal Local Diversity of Marseilleviridae. Frontiers in Microbiology, 10, 1152. https://doi.org/10.3389/fmicb.2019.01152
Aoki K, et al. Fifteen Marseilleviruses Newly Isolated From Three Water Samples in Japan Reveal Local Diversity of Marseilleviridae. Front Microbiol. 2019;10:1152. PubMed PMID: 31178850.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fifteen Marseilleviruses Newly Isolated From Three Water Samples in Japan Reveal Local Diversity of Marseilleviridae. AU - Aoki,Keita, AU - Hagiwara,Reika, AU - Akashi,Motohiro, AU - Sasaki,Kenta, AU - Murata,Kazuyoshi, AU - Ogata,Hiroyuki, AU - Takemura,Masaharu, Y1 - 2019/05/24/ PY - 2019/03/07/received PY - 2019/05/06/accepted PY - 2019/6/11/entrez PY - 2019/6/11/pubmed PY - 2019/6/11/medline KW - family Marseilleviridae KW - giant viruses KW - isolation KW - major capsid protein KW - nucleo-cytoplasmic large DNA virus KW - phylogenetic analysis SP - 1152 EP - 1152 JF - Frontiers in microbiology JO - Front Microbiol VL - 10 N2 - The family Marseilleviridae, defined as a group of icosahedral double-stranded DNA viruses with particle size of approximately 250 nm and genome size of 350-380 kbp, belongs to the nucleo-cytoplasmic family of large DNA viruses. The family Marseilleviridae is currently classified into lineages A-E. In this study, we isolated 12 or 15 new members of the family Marseilleviridae from three sampling locations in Japan. Molecular phylogenetic analysis of the MCP genes showed that the new viruses could be further classified into three groups, hokutoviruses, kashiwazakiviruses, and kyotoviruses. Hokutoviruses were closely related to lineage B, kyotoviruses were related to lineage A, and kashiwazakiviruses were also classified into lineage B but a new putative subgroup of lineage B, revealing the diversity of this lineage. Interestingly, more than two viruses with slightly different MCP genes were isolated from a single water sample from a single location, i.e., two hokutoviruses and one kashiwazakivirus were isolated from a small reservoir, five kashiwazakiviruses from the mouth of a river, and five kyotoviruses from fresh water of a river, suggesting that several milliliters of water samples contain several types of giant viruses. Amoeba cells infected with hokutoviruses or kashiwazakiviruses exhibited a "bunch" formation consisting of normal and infected cells similarly to a tupanvirus, whereas cells infected with kyotoviruses or tokyovirus did not. These results suggest the previously unrecognized local diversity of the family Marseilleviridae in aquatic environments. SN - 1664-302X UR - https://www.unboundmedicine.com/medline/citation/31178850/Fifteen_Marseilleviruses_Newly_Isolated_From_Three_Water_Samples_in_Japan_Reveal_Local_Diversity_of_Marseilleviridae_ L2 - https://doi.org/10.3389/fmicb.2019.01152 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.