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The effect of nutrient restriction and syndecan-4 or glypican-1 knockdown on the differentiation of turkey pectoralis major satellite cells differing in age and growth selection.
Poult Sci. 2019 Nov 01; 98(11):6078-6090.PS

Abstract

Skeletal muscle growth is mediated by the proliferation and differentiation of satellite cells, whose activity is affected by both nutrition and the expression of syndecan-4 and glypican-1. Previous research has not addressed if there is an interactive effect of nutrition with the expression of syndecan-4 and glypican-1. Thus, the objective of the current study was to determine if the response of satellite cells to nutrient restriction was altered by syndecan-4 or glypican-1 knockdown and if age and growth selection are factors. Satellite cells were isolated from pectoralis major muscle of 1-day, 7-wk, and 16-wk-old turkeys selected for increased 16-wk body weight (F line) and the randombred control (RBC2) line from which the F line was selected. Syndecan-4 or glypican-1 expression was knocked down in both lines using small interfering RNAs along with nutrient restriction of 0 or 20% of the standard cell culture medium either applied during proliferation with subsequent normal differentiation medium (RN) or during differentiation with preceding normal proliferation medium (NR). For both lines, nutrient restriction and syndecan-4 or glypican-1 knockdown had an independent and additive effect on satellite cell differentiation at 72 h of differentiation except for 1 d satellite cells. The 1 d satellite cell differentiation was increased by RN treatment, but when combined with syndecan-4 or glypican-1 knockdown, the increase in differentiation was negated. At 48 h of differentiation, syndecan-4 knockdown in 7 and 16 wk satellite cells and glypican-1 knockdown in 7 wk cells cancelled the effect of the RN treatment, but enhanced the effect of NR treatment at 24 h of differentiation. Growth selection had little effect on the interaction between nutrient restriction and syndecan-4 or glypican-1 knockdown. Taken together, these data demonstrate that the satellite cell response to nutrition is dependent on the expression of syndecan-4 and glypican-1 in an age-dependent manner with growth selection having little impact.

Authors+Show Affiliations

The Ohio State University/Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA.The Ohio State University/Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA.The Ohio State University/Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31180126

Citation

Velleman, Sandra G., et al. "The Effect of Nutrient Restriction and Syndecan-4 or Glypican-1 Knockdown On the Differentiation of Turkey Pectoralis Major Satellite Cells Differing in Age and Growth Selection." Poultry Science, vol. 98, no. 11, 2019, pp. 6078-6090.
Velleman SG, Clark DL, Tonniges JR. The effect of nutrient restriction and syndecan-4 or glypican-1 knockdown on the differentiation of turkey pectoralis major satellite cells differing in age and growth selection. Poult Sci. 2019;98(11):6078-6090.
Velleman, S. G., Clark, D. L., & Tonniges, J. R. (2019). The effect of nutrient restriction and syndecan-4 or glypican-1 knockdown on the differentiation of turkey pectoralis major satellite cells differing in age and growth selection. Poultry Science, 98(11), 6078-6090. https://doi.org/10.3382/ps/pez304
Velleman SG, Clark DL, Tonniges JR. The Effect of Nutrient Restriction and Syndecan-4 or Glypican-1 Knockdown On the Differentiation of Turkey Pectoralis Major Satellite Cells Differing in Age and Growth Selection. Poult Sci. 2019 Nov 1;98(11):6078-6090. PubMed PMID: 31180126.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of nutrient restriction and syndecan-4 or glypican-1 knockdown on the differentiation of turkey pectoralis major satellite cells differing in age and growth selection. AU - Velleman,Sandra G, AU - Clark,Daniel L, AU - Tonniges,Jeffrey R, PY - 2019/01/02/received PY - 2019/05/16/accepted PY - 2019/6/11/pubmed PY - 2020/1/9/medline PY - 2019/6/11/entrez KW - glypican-1 KW - nutrition KW - satellite cell KW - skeletal muscle KW - syndecan-4 SP - 6078 EP - 6090 JF - Poultry science JO - Poult Sci VL - 98 IS - 11 N2 - Skeletal muscle growth is mediated by the proliferation and differentiation of satellite cells, whose activity is affected by both nutrition and the expression of syndecan-4 and glypican-1. Previous research has not addressed if there is an interactive effect of nutrition with the expression of syndecan-4 and glypican-1. Thus, the objective of the current study was to determine if the response of satellite cells to nutrient restriction was altered by syndecan-4 or glypican-1 knockdown and if age and growth selection are factors. Satellite cells were isolated from pectoralis major muscle of 1-day, 7-wk, and 16-wk-old turkeys selected for increased 16-wk body weight (F line) and the randombred control (RBC2) line from which the F line was selected. Syndecan-4 or glypican-1 expression was knocked down in both lines using small interfering RNAs along with nutrient restriction of 0 or 20% of the standard cell culture medium either applied during proliferation with subsequent normal differentiation medium (RN) or during differentiation with preceding normal proliferation medium (NR). For both lines, nutrient restriction and syndecan-4 or glypican-1 knockdown had an independent and additive effect on satellite cell differentiation at 72 h of differentiation except for 1 d satellite cells. The 1 d satellite cell differentiation was increased by RN treatment, but when combined with syndecan-4 or glypican-1 knockdown, the increase in differentiation was negated. At 48 h of differentiation, syndecan-4 knockdown in 7 and 16 wk satellite cells and glypican-1 knockdown in 7 wk cells cancelled the effect of the RN treatment, but enhanced the effect of NR treatment at 24 h of differentiation. Growth selection had little effect on the interaction between nutrient restriction and syndecan-4 or glypican-1 knockdown. Taken together, these data demonstrate that the satellite cell response to nutrition is dependent on the expression of syndecan-4 and glypican-1 in an age-dependent manner with growth selection having little impact. SN - 1525-3171 UR - https://www.unboundmedicine.com/medline/citation/31180126/The_effect_of_nutrient_restriction_and_syndecan_4_or_glypican_1_knockdown_on_the_differentiation_of_turkey_pectoralis_major_satellite_cells_differing_in_age_and_growth_selection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0032-5791(19)45815-X DB - PRIME DP - Unbound Medicine ER -