Tags

Type your tag names separated by a space and hit enter

Blockade of spinal α5-GABAA receptors differentially reduces reserpine-induced fibromyalgia-type pain in female rats.
Eur J Pharmacol 2019; 858:172443EJ

Abstract

The role of spinal α5 subunit-containing GABAA (α5-GABAA) receptors in chronic pain is controversial. The purpose of this study was to investigate the participation of spinal α5-GABAA receptors in the reserpine-induced pain model. Reserpine administration induced tactile allodynia and muscle hyperalgesia in female and male rats. Intrathecal injection of L-655,708 and TB 21007 (7 days after the last reserpine injection) decreased tactile allodynia and, at a lesser extent, muscle hyperalgesia in female rats. The effects of these drugs produced a lower antiallodynic and antihyperalgesic effect in male than in female rats. Contrariwise, these drugs produced tactile allodynia and muscle hyperalgesia in naïve rats and these effects were lower in naïve male than female rats. Intrathecal L-838,417 prevented or reversed L-655,708-induced antiallodynia in reserpine-treated female rats. Repeated treatment with α5-GABAA receptor small interfering RNA (siRNA), but not scramble siRNA, reduced reserpine-induced allodynia in female rats. Accordingly, α5-GABAA receptor siRNA induced nociceptive hypersensitivity in naïve female rats. Reserpine enhanced α5-GABAA receptors expression in spinal cord and dorsal root ganglia (DRG), while it increased CD11b (OX-42) and glial fibrillary acidic protein (GFAP) fluorescence intensity in the lumbar spinal cord. In contrast, reserpine diminished K+-Cl- co-transporter 2 (KCC2) protein in the lumbar spinal cord. Data suggest that spinal α5-GABAA receptors play a sex-dependent proallodynic effect in reserpine-treated rats. In contrast, these receptors have a sex-dependent antiallodynic role in naïve rats.

Authors+Show Affiliations

Neurobiology of Pain Laboratory, Departamento de Farmacobiología, Cinvestav, Sede Sur, Mexico City, Mexico.Neurobiology of Pain Laboratory, Departamento de Farmacobiología, Cinvestav, Sede Sur, Mexico City, Mexico.Neurobiology of Pain Laboratory, Departamento de Farmacobiología, Cinvestav, Sede Sur, Mexico City, Mexico.Neurobiology of Pain Laboratory, Departamento de Farmacobiología, Cinvestav, Sede Sur, Mexico City, Mexico.Departamento de Fisiología, Biofísica y Neurociencias, Cinvestav, Mexico City, Mexico.Neurobiology of Pain Laboratory, Departamento de Farmacobiología, Cinvestav, Sede Sur, Mexico City, Mexico. Electronic address: vgranados@cinvestav.mx.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31181208

Citation

De la Luz-Cuellar, Yarim E., et al. "Blockade of Spinal α5-GABAA Receptors Differentially Reduces Reserpine-induced Fibromyalgia-type Pain in Female Rats." European Journal of Pharmacology, vol. 858, 2019, p. 172443.
De la Luz-Cuellar YE, Rodríguez-Palma EJ, Franco-Enzástiga Ú, et al. Blockade of spinal α5-GABAA receptors differentially reduces reserpine-induced fibromyalgia-type pain in female rats. Eur J Pharmacol. 2019;858:172443.
De la Luz-Cuellar, Y. E., Rodríguez-Palma, E. J., Franco-Enzástiga, Ú., Salinas-Abarca, A. B., Delgado-Lezama, R., & Granados-Soto, V. (2019). Blockade of spinal α5-GABAA receptors differentially reduces reserpine-induced fibromyalgia-type pain in female rats. European Journal of Pharmacology, 858, p. 172443. doi:10.1016/j.ejphar.2019.172443.
De la Luz-Cuellar YE, et al. Blockade of Spinal α5-GABAA Receptors Differentially Reduces Reserpine-induced Fibromyalgia-type Pain in Female Rats. Eur J Pharmacol. 2019 Sep 5;858:172443. PubMed PMID: 31181208.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Blockade of spinal α5-GABAA receptors differentially reduces reserpine-induced fibromyalgia-type pain in female rats. AU - De la Luz-Cuellar,Yarim E, AU - Rodríguez-Palma,Erick J, AU - Franco-Enzástiga,Úrzula, AU - Salinas-Abarca,Ana B, AU - Delgado-Lezama,Rodolfo, AU - Granados-Soto,Vinicio, Y1 - 2019/06/07/ PY - 2018/12/06/received PY - 2019/05/17/revised PY - 2019/06/06/accepted PY - 2019/6/11/pubmed PY - 2019/6/11/medline PY - 2019/6/11/entrez KW - Disinhibition KW - Fibromyalgia KW - Functional pain KW - α(5)-GABA(A) receptors SP - 172443 EP - 172443 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 858 N2 - The role of spinal α5 subunit-containing GABAA (α5-GABAA) receptors in chronic pain is controversial. The purpose of this study was to investigate the participation of spinal α5-GABAA receptors in the reserpine-induced pain model. Reserpine administration induced tactile allodynia and muscle hyperalgesia in female and male rats. Intrathecal injection of L-655,708 and TB 21007 (7 days after the last reserpine injection) decreased tactile allodynia and, at a lesser extent, muscle hyperalgesia in female rats. The effects of these drugs produced a lower antiallodynic and antihyperalgesic effect in male than in female rats. Contrariwise, these drugs produced tactile allodynia and muscle hyperalgesia in naïve rats and these effects were lower in naïve male than female rats. Intrathecal L-838,417 prevented or reversed L-655,708-induced antiallodynia in reserpine-treated female rats. Repeated treatment with α5-GABAA receptor small interfering RNA (siRNA), but not scramble siRNA, reduced reserpine-induced allodynia in female rats. Accordingly, α5-GABAA receptor siRNA induced nociceptive hypersensitivity in naïve female rats. Reserpine enhanced α5-GABAA receptors expression in spinal cord and dorsal root ganglia (DRG), while it increased CD11b (OX-42) and glial fibrillary acidic protein (GFAP) fluorescence intensity in the lumbar spinal cord. In contrast, reserpine diminished K+-Cl- co-transporter 2 (KCC2) protein in the lumbar spinal cord. Data suggest that spinal α5-GABAA receptors play a sex-dependent proallodynic effect in reserpine-treated rats. In contrast, these receptors have a sex-dependent antiallodynic role in naïve rats. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/31181208/Blockade_of_spinal_α5-GABAA_receptors_differentially_reduces_reserpine-induced_fibromyalgia-type_pain_in_female_rats L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(19)30394-2 DB - PRIME DP - Unbound Medicine ER -