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Anti-hyperuricemic effect of Alpinia oxyphylla seed extract by enhancing uric acid excretion in the kidney.
Phytomedicine. 2019 Sep; 62:152975.P

Abstract

BACKGROUND

Alpinia oxyphylla is a well-known traditional medicine used in China and Korea to treat intestinal disorders, urosis, diuresis, and chronic glomerulonephritis.

PURPOSE

We investigated the anti-hyperuricemic effects of Alpinia oxyphylla seed extract (AE), and the underlying mechanisms of action through in vitro and in vivo studies.

METHODS

We evaluated levels of uric acid in the serum and urine, the expression of renal urate transport proteins, and levels of inflammatory cytokines in potassium oxonate (PO)-induced hyperuricemic rats. Xanthine oxidase activity was analyzed in vitro, while cellular uric acid uptake was assessed in oocytes expressing the human urate transporter 1 (hURAT1). Moreover, the main components of AE were analyzed using UPLC.

RESULTS

In PO-induced hyperuricemic rats, 200 and 400 mg/kg of AE significantly decreased levels of uric acid in serum, while 400 mg/kg of AE increased uric acid levels in urine. AE did not inhibit xanthine oxidase in vitro; however, 1, 10, and 100 μg/ml of AE significantly decreased uric acid uptake into oocytes expressing hURAT1. Furthermore, 400 mg/kg of AE increased levels of organic anion transporter (OAT) 1 protein, while 200 and 400 mg/kg of AE decreased the protein content of urate transporter, URAT1 and inflammatory cytokines in the kidneys. Nootkatone was identified as one the main chemical components in AE from UPLC analysis.

CONCLUSIONS

These findings suggest that AE exerts anti-hyperuricemic and uricosuric effects, which are related to the promotion of uric acid excretion via enhanced secretion and inhibition of uric acid reabsorption in the kidneys. Thus, AE may be a potential treatment for hyperuricemia and gout.

Authors+Show Affiliations

Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Dajeon 34054, Republic of Korea.Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Dajeon 34054, Republic of Korea.Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Dajeon 34054, Republic of Korea.Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Dajeon 34054, Republic of Korea.R&D Research innovation Team, Kwangdong Pharm Co., Ltd. 271, Digital-ro, Guro-gu, Seoul 08387, Republic of Korea.R&D Research innovation Team, Kwangdong Pharm Co., Ltd. 271, Digital-ro, Guro-gu, Seoul 08387, Republic of Korea.Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Dajeon 34054, Republic of Korea. Electronic address: dskim@kiom.re.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31181404

Citation

Lee, Young-Sil, et al. "Anti-hyperuricemic Effect of Alpinia Oxyphylla Seed Extract By Enhancing Uric Acid Excretion in the Kidney." Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, vol. 62, 2019, p. 152975.
Lee YS, Sung YY, Yuk HJ, et al. Anti-hyperuricemic effect of Alpinia oxyphylla seed extract by enhancing uric acid excretion in the kidney. Phytomedicine. 2019;62:152975.
Lee, Y. S., Sung, Y. Y., Yuk, H. J., Son, E., Lee, S., Kim, J. S., & Kim, D. S. (2019). Anti-hyperuricemic effect of Alpinia oxyphylla seed extract by enhancing uric acid excretion in the kidney. Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 62, 152975. https://doi.org/10.1016/j.phymed.2019.152975
Lee YS, et al. Anti-hyperuricemic Effect of Alpinia Oxyphylla Seed Extract By Enhancing Uric Acid Excretion in the Kidney. Phytomedicine. 2019;62:152975. PubMed PMID: 31181404.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-hyperuricemic effect of Alpinia oxyphylla seed extract by enhancing uric acid excretion in the kidney. AU - Lee,Young-Sil, AU - Sung,Yoon-Young, AU - Yuk,Heung Joo, AU - Son,Eunjung, AU - Lee,SeonJoo, AU - Kim,Jin Soo, AU - Kim,Dong-Seon, Y1 - 2019/05/31/ PY - 2018/10/19/received PY - 2019/05/15/revised PY - 2019/05/30/accepted PY - 2019/6/11/pubmed PY - 2020/3/25/medline PY - 2019/6/11/entrez KW - Alpinia oxyphylla Miquel KW - Hyperuricemia KW - Organic anion transporter 1 (OAT1) KW - Potassium oxonate-induced hyperuricemic rat KW - Urate transporter 1 (URAT1) KW - Uric acid excretion SP - 152975 EP - 152975 JF - Phytomedicine : international journal of phytotherapy and phytopharmacology JO - Phytomedicine VL - 62 N2 - BACKGROUND: Alpinia oxyphylla is a well-known traditional medicine used in China and Korea to treat intestinal disorders, urosis, diuresis, and chronic glomerulonephritis. PURPOSE: We investigated the anti-hyperuricemic effects of Alpinia oxyphylla seed extract (AE), and the underlying mechanisms of action through in vitro and in vivo studies. METHODS: We evaluated levels of uric acid in the serum and urine, the expression of renal urate transport proteins, and levels of inflammatory cytokines in potassium oxonate (PO)-induced hyperuricemic rats. Xanthine oxidase activity was analyzed in vitro, while cellular uric acid uptake was assessed in oocytes expressing the human urate transporter 1 (hURAT1). Moreover, the main components of AE were analyzed using UPLC. RESULTS: In PO-induced hyperuricemic rats, 200 and 400 mg/kg of AE significantly decreased levels of uric acid in serum, while 400 mg/kg of AE increased uric acid levels in urine. AE did not inhibit xanthine oxidase in vitro; however, 1, 10, and 100 μg/ml of AE significantly decreased uric acid uptake into oocytes expressing hURAT1. Furthermore, 400 mg/kg of AE increased levels of organic anion transporter (OAT) 1 protein, while 200 and 400 mg/kg of AE decreased the protein content of urate transporter, URAT1 and inflammatory cytokines in the kidneys. Nootkatone was identified as one the main chemical components in AE from UPLC analysis. CONCLUSIONS: These findings suggest that AE exerts anti-hyperuricemic and uricosuric effects, which are related to the promotion of uric acid excretion via enhanced secretion and inhibition of uric acid reabsorption in the kidneys. Thus, AE may be a potential treatment for hyperuricemia and gout. SN - 1618-095X UR - https://www.unboundmedicine.com/medline/citation/31181404/Anti_hyperuricemic_effect_of_Alpinia_oxyphylla_seed_extract_by_enhancing_uric_acid_excretion_in_the_kidney_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-7113(19)30142-4 DB - PRIME DP - Unbound Medicine ER -