Tags

Type your tag names separated by a space and hit enter

Molecular characterization of enterotoxigenic Escherichia coli isolated from neonatal calves diarrhea.
Vet Res Forum 2019; 10(1):73-78VR

Abstract

Enterotoxigenic Escherichia coli (ETEC) produces different virulence factors allowing the bacterium to colonize and develop watery diarrhea. Proteomics studies have also introduced new protein belonging to the secretion pathways, antigen 43 (Ag43), which plays important role in E. coli pathogenesis. The objective of this study was to investigate O-types and virulence factors of E. coli isolates from neonatal calves diarrhea. Total of 120 isolates from diarrheic calves were genotyped for their O groups and the presence of virulence genes K99, F41 and STa as well as Ag43. The predominant O-type was O101 (51.00%) and the prevalence of K99, F41 and STa was 7 (5.80%). The Ag43 was detected in all samples with three different allelic patterns. Our results indicated that K99 positive isolates certainly have one of each 2200 bp or 1800 bp or both copies of Ag43 passenger domain, while negative K99 isolates lack the Ag43. The results reported here provide informative data regarding the prevalence of E. coli O-types and their virulence factors in enteric colibacillosis. The Ag43 that was more found in K99 positive isolates might be associated with diarrhea-causing E. coli strains in neonatal calves.

Authors+Show Affiliations

Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.Department of Microbiology, Faculty of Veterinary Medicine, Branch of Karaj, Islamic Azad University, Karaj, Iran.Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31183019

Citation

Yadegari, Zahra, et al. "Molecular Characterization of Enterotoxigenic Escherichia Coli Isolated From Neonatal Calves Diarrhea." Veterinary Research Forum : an International Quarterly Journal, vol. 10, no. 1, 2019, pp. 73-78.
Yadegari Z, Nikbakht Brujeni G, Ghorbanpour R, et al. Molecular characterization of enterotoxigenic Escherichia coli isolated from neonatal calves diarrhea. Vet Res Forum. 2019;10(1):73-78.
Yadegari, Z., Nikbakht Brujeni, G., Ghorbanpour, R., Moosakhani, F., & Lotfollahzadeh, S. (2019). Molecular characterization of enterotoxigenic Escherichia coli isolated from neonatal calves diarrhea. Veterinary Research Forum : an International Quarterly Journal, 10(1), pp. 73-78. doi:10.30466/vrf.2019.34313.
Yadegari Z, et al. Molecular Characterization of Enterotoxigenic Escherichia Coli Isolated From Neonatal Calves Diarrhea. Vet Res Forum. 2019;10(1):73-78. PubMed PMID: 31183019.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of enterotoxigenic Escherichia coli isolated from neonatal calves diarrhea. AU - Yadegari,Zahra, AU - Nikbakht Brujeni,Gholamreza, AU - Ghorbanpour,Reyhaneh, AU - Moosakhani,Farhad, AU - Lotfollahzadeh,Samad, Y1 - 2019/03/15/ PY - 2018/02/13/received PY - 2018/07/30/accepted PY - 2019/6/12/entrez PY - 2019/6/12/pubmed PY - 2019/6/12/medline KW - Antigen 43 KW - Calf KW - Diarrhea KW - Entrotoxigenic Escherichia coli KW - O-type SP - 73 EP - 78 JF - Veterinary research forum : an international quarterly journal JO - Vet Res Forum VL - 10 IS - 1 N2 - Enterotoxigenic Escherichia coli (ETEC) produces different virulence factors allowing the bacterium to colonize and develop watery diarrhea. Proteomics studies have also introduced new protein belonging to the secretion pathways, antigen 43 (Ag43), which plays important role in E. coli pathogenesis. The objective of this study was to investigate O-types and virulence factors of E. coli isolates from neonatal calves diarrhea. Total of 120 isolates from diarrheic calves were genotyped for their O groups and the presence of virulence genes K99, F41 and STa as well as Ag43. The predominant O-type was O101 (51.00%) and the prevalence of K99, F41 and STa was 7 (5.80%). The Ag43 was detected in all samples with three different allelic patterns. Our results indicated that K99 positive isolates certainly have one of each 2200 bp or 1800 bp or both copies of Ag43 passenger domain, while negative K99 isolates lack the Ag43. The results reported here provide informative data regarding the prevalence of E. coli O-types and their virulence factors in enteric colibacillosis. The Ag43 that was more found in K99 positive isolates might be associated with diarrhea-causing E. coli strains in neonatal calves. SN - 2008-8140 UR - https://www.unboundmedicine.com/medline/citation/31183019/Molecular_characterization_of_enterotoxigenic_Escherichia_coli_isolated_from_neonatal_calves_diarrhea_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/31183019/ DB - PRIME DP - Unbound Medicine ER -