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Long noncoding RNA expression analysis reveals the regulatory effects of nitinol-based nanotubular coatings on human coronary artery endothelial cells.
Int J Nanomedicine. 2019; 14:3297-3309.IJ

Abstract

Background:

Cardiovascular disease (CVD) is the leading cause of mortality all over the world. Vascular stents are used to ameliorate vascular stenosis and recover vascular function. The application of nanotubular coatings has been confirmed to promote endothelial cell (EC) proliferation and function. However, the regulatory mechanisms involved in cellular responses to the nanotubular topography have not been defined. In the present study, a microarray analysis was performed to explore the expression patterns of long noncoding RNAs (lncRNAs) in human coronary artery endothelial cells (HCAECs) that were differentially expressed in response to nitinol-based nanotubular coatings. Materials and methods: First, anodization was performed to synthesize nitinol-based nanotubular coatings. Then, HCAECs were cultured on the samples for 24 h to evaluate cell cytoskeleton organization. Next, total RNA was extracted and synthesized into cRNA, which was hybridized onto the microarray. GO analysis and KEGG pathway analysis were performed to investigate the roles of differentially expressed messenger RNAs (mRNAs). Quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) was performed to validate the expression of randomly selected lncRNAs. Coexpression networks were created to identify the interactions among lncRNAs and the protein-coding genes involved in nanotubular topography-induced biological and molecular pathways. Independent Student's t-test was applied for comparisons between two groups with statistical significance set at p<0.05.

Results:

1085 lncRNAs and 227 mRNAs were significantly differentially expressed in the nitinol-based nanotubular coating group. Bioinformatics analysis revealed that extracellular matrix receptor interactions and cell adhesion molecules play critical roles in the sensing of nitinol-based nanotubular coatings by HCAECs. The TATA-binding protein (TBP) and TBP-associated transfactor 1 (TAF1) are important molecules in EC responses to substrate topography.

Conclusion:

This study suggests that nanotubular substrate topography regulates ECs by differentially expressed lncRNAs involved extracellular matrix receptor interactions and cell adhesion molecules.

Authors+Show Affiliations

Academy of Military Medical Sciences, Institute of Health Service and Transfusion Medicine, Beijing 100850, People's Republic of China.Academy of Military Medical Sciences, Institute of Health Service and Transfusion Medicine, Beijing 100850, People's Republic of China.Photoelectrochemical Research Group, School of Materials Science and Engineering, Beijing University of Technology, Beijing 100124, People's Republic of China.Academy of Military Medical Sciences, Institute of Health Service and Transfusion Medicine, Beijing 100850, People's Republic of China.Photoelectrochemical Research Group, School of Materials Science and Engineering, Beijing University of Technology, Beijing 100124, People's Republic of China.Academy of Military Medical Sciences, Institute of Health Service and Transfusion Medicine, Beijing 100850, People's Republic of China.Academy of Military Medical Sciences, Institute of Health Service and Transfusion Medicine, Beijing 100850, People's Republic of China.Photoelectrochemical Research Group, School of Materials Science and Engineering, Beijing University of Technology, Beijing 100124, People's Republic of China.Academy of Military Medical Sciences, Institute of Health Service and Transfusion Medicine, Beijing 100850, People's Republic of China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31190794

Citation

Shang, Pan, et al. "Long Noncoding RNA Expression Analysis Reveals the Regulatory Effects of Nitinol-based Nanotubular Coatings On Human Coronary Artery Endothelial Cells." International Journal of Nanomedicine, vol. 14, 2019, pp. 3297-3309.
Shang P, Chen G, Zu G, et al. Long noncoding RNA expression analysis reveals the regulatory effects of nitinol-based nanotubular coatings on human coronary artery endothelial cells. Int J Nanomedicine. 2019;14:3297-3309.
Shang, P., Chen, G., Zu, G., Song, X., Jiao, P., You, G., Zhao, J., Li, H., & Zhou, H. (2019). Long noncoding RNA expression analysis reveals the regulatory effects of nitinol-based nanotubular coatings on human coronary artery endothelial cells. International Journal of Nanomedicine, 14, 3297-3309. https://doi.org/10.2147/IJN.S204067
Shang P, et al. Long Noncoding RNA Expression Analysis Reveals the Regulatory Effects of Nitinol-based Nanotubular Coatings On Human Coronary Artery Endothelial Cells. Int J Nanomedicine. 2019;14:3297-3309. PubMed PMID: 31190794.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long noncoding RNA expression analysis reveals the regulatory effects of nitinol-based nanotubular coatings on human coronary artery endothelial cells. AU - Shang,Pan, AU - Chen,Gan, AU - Zu,Guannan, AU - Song,Xiang, AU - Jiao,Peng, AU - You,Guoxing, AU - Zhao,Jingxiang, AU - Li,Hongyi, AU - Zhou,Hong, Y1 - 2019/05/07/ PY - 2019/02/03/received PY - 2019/04/04/accepted PY - 2019/6/14/entrez PY - 2019/6/14/pubmed PY - 2019/8/3/medline KW - RNA sequencing KW - long noncoding RNA KW - molecular networks KW - nanotopography KW - nitinol titanium dioxide nanotubes SP - 3297 EP - 3309 JF - International journal of nanomedicine JO - Int J Nanomedicine VL - 14 N2 - Background: Cardiovascular disease (CVD) is the leading cause of mortality all over the world. Vascular stents are used to ameliorate vascular stenosis and recover vascular function. The application of nanotubular coatings has been confirmed to promote endothelial cell (EC) proliferation and function. However, the regulatory mechanisms involved in cellular responses to the nanotubular topography have not been defined. In the present study, a microarray analysis was performed to explore the expression patterns of long noncoding RNAs (lncRNAs) in human coronary artery endothelial cells (HCAECs) that were differentially expressed in response to nitinol-based nanotubular coatings. Materials and methods: First, anodization was performed to synthesize nitinol-based nanotubular coatings. Then, HCAECs were cultured on the samples for 24 h to evaluate cell cytoskeleton organization. Next, total RNA was extracted and synthesized into cRNA, which was hybridized onto the microarray. GO analysis and KEGG pathway analysis were performed to investigate the roles of differentially expressed messenger RNAs (mRNAs). Quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) was performed to validate the expression of randomly selected lncRNAs. Coexpression networks were created to identify the interactions among lncRNAs and the protein-coding genes involved in nanotubular topography-induced biological and molecular pathways. Independent Student's t-test was applied for comparisons between two groups with statistical significance set at p<0.05. Results: 1085 lncRNAs and 227 mRNAs were significantly differentially expressed in the nitinol-based nanotubular coating group. Bioinformatics analysis revealed that extracellular matrix receptor interactions and cell adhesion molecules play critical roles in the sensing of nitinol-based nanotubular coatings by HCAECs. The TATA-binding protein (TBP) and TBP-associated transfactor 1 (TAF1) are important molecules in EC responses to substrate topography. Conclusion: This study suggests that nanotubular substrate topography regulates ECs by differentially expressed lncRNAs involved extracellular matrix receptor interactions and cell adhesion molecules. SN - 1178-2013 UR - https://www.unboundmedicine.com/medline/citation/31190794/Long_noncoding_RNA_expression_analysis_reveals_the_regulatory_effects_of_nitinol_based_nanotubular_coatings_on_human_coronary_artery_endothelial_cells_ L2 - https://dx.doi.org/10.2147/IJN.S204067 DB - PRIME DP - Unbound Medicine ER -