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CircRNA-9119 suppresses poly I:C induced inflammation in Leydig and Sertoli cells via TLR3 and RIG-I signal pathways.
Mol Med. 2019 06 13; 25(1):28.MM

Abstract

BACKGROUND

Circular RNAs (circRNAs) contribute to the epigenetic modulation of pathological and physiological conditions. The understanding of the impact of circRNAs on generation of testicular inflammatory reactions is insufficient.

METHODS

Our research adopted a poly I:C-triggered testicular inflammation murine model and cell assays.

RESULTS

Microarray data and quantitative evaluation revealed the elevation in the concentrations of Toll-like receptor 3 (TLR3), circRNA-9119, and retinoic acid inducible gene-I (RIG-I) and repression in the levels of miR-136 and miR-26a. Inhibition of circRNA-9119 expression impaired the inflammatory reactions in the separated Leydig and Sertoli cells subjected to poly I:C treatment. CircRNA-9119 suppressed the expression of miR-136 and miR-26a by acting as a microRNA sponge. miR-136 and miR-26a repressed the expression of RIG-I and TLR3 through the expected target region in Leydig and Sertoli cells in vitro. Inhibition of miR-136 and miR-26a expression, at least in part, restored the expression of inflammatory cytokines, which were inhibited upon circRNA-9119 expression silencing. Furthermore, the expression of circRNA-9119 was positively associated with RIG-I and TLR3 mRNA and protein levels. The expression of inflammatory genes triggered by poly I:C treatment was noticeably suppressed after RIG-I and TLR3 knockout.

CONCLUSIONS

Our results suggest that circRNA-9119 may serve as a competing endogenous RNA that insulated miR-136 and miR-26a and consequently defended RIG-I and TLR3 mRNAs against miR-26a/miR-136-mediated inhibition of testicular cells. Moreover, RIG-I and TLR3 contributed to the modulation of poly I:C-triggered inflammatory cytokine generation during orchitis in testicular cells.

Authors+Show Affiliations

Department of Pediatric Surgery, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Zhejiang, Wenzhou, China.Department of Pediatric Surgery, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Zhejiang, Wenzhou, China.Department of Radiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, No. 109, West College Road, Lucheng District, Wenzhou, 325027, Zhejiang, China. xiaoxiaoxieyx@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31195953

Citation

Qin, Le, et al. "CircRNA-9119 Suppresses Poly I:C Induced Inflammation in Leydig and Sertoli Cells Via TLR3 and RIG-I Signal Pathways." Molecular Medicine (Cambridge, Mass.), vol. 25, no. 1, 2019, p. 28.
Qin L, Lin J, Xie X. CircRNA-9119 suppresses poly I:C induced inflammation in Leydig and Sertoli cells via TLR3 and RIG-I signal pathways. Mol Med. 2019;25(1):28.
Qin, L., Lin, J., & Xie, X. (2019). CircRNA-9119 suppresses poly I:C induced inflammation in Leydig and Sertoli cells via TLR3 and RIG-I signal pathways. Molecular Medicine (Cambridge, Mass.), 25(1), 28. https://doi.org/10.1186/s10020-019-0094-1
Qin L, Lin J, Xie X. CircRNA-9119 Suppresses Poly I:C Induced Inflammation in Leydig and Sertoli Cells Via TLR3 and RIG-I Signal Pathways. Mol Med. 2019 06 13;25(1):28. PubMed PMID: 31195953.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CircRNA-9119 suppresses poly I:C induced inflammation in Leydig and Sertoli cells via TLR3 and RIG-I signal pathways. AU - Qin,Le, AU - Lin,Jie, AU - Xie,Xiaoxiao, Y1 - 2019/06/13/ PY - 2019/01/14/received PY - 2019/05/21/accepted PY - 2019/6/15/entrez PY - 2019/6/15/pubmed PY - 2020/6/23/medline KW - Inflammation KW - Orchitis KW - RIG-I KW - TLR3 KW - circRNA-9119 KW - miR-136 KW - miR-26a SP - 28 EP - 28 JF - Molecular medicine (Cambridge, Mass.) JO - Mol. Med. VL - 25 IS - 1 N2 - BACKGROUND: Circular RNAs (circRNAs) contribute to the epigenetic modulation of pathological and physiological conditions. The understanding of the impact of circRNAs on generation of testicular inflammatory reactions is insufficient. METHODS: Our research adopted a poly I:C-triggered testicular inflammation murine model and cell assays. RESULTS: Microarray data and quantitative evaluation revealed the elevation in the concentrations of Toll-like receptor 3 (TLR3), circRNA-9119, and retinoic acid inducible gene-I (RIG-I) and repression in the levels of miR-136 and miR-26a. Inhibition of circRNA-9119 expression impaired the inflammatory reactions in the separated Leydig and Sertoli cells subjected to poly I:C treatment. CircRNA-9119 suppressed the expression of miR-136 and miR-26a by acting as a microRNA sponge. miR-136 and miR-26a repressed the expression of RIG-I and TLR3 through the expected target region in Leydig and Sertoli cells in vitro. Inhibition of miR-136 and miR-26a expression, at least in part, restored the expression of inflammatory cytokines, which were inhibited upon circRNA-9119 expression silencing. Furthermore, the expression of circRNA-9119 was positively associated with RIG-I and TLR3 mRNA and protein levels. The expression of inflammatory genes triggered by poly I:C treatment was noticeably suppressed after RIG-I and TLR3 knockout. CONCLUSIONS: Our results suggest that circRNA-9119 may serve as a competing endogenous RNA that insulated miR-136 and miR-26a and consequently defended RIG-I and TLR3 mRNAs against miR-26a/miR-136-mediated inhibition of testicular cells. Moreover, RIG-I and TLR3 contributed to the modulation of poly I:C-triggered inflammatory cytokine generation during orchitis in testicular cells. SN - 1528-3658 UR - https://www.unboundmedicine.com/medline/citation/31195953/CircRNA_9119_suppresses_poly_I:C_induced_inflammation_in_Leydig_and_Sertoli_cells_via_TLR3_and_RIG_I_signal_pathways_ L2 - https://doi.org/10.1186/s10020-019-0094-1 DB - PRIME DP - Unbound Medicine ER -