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Differential gene expression in articular cartilage between rheumatoid arthritis and endemic Kashin-Beck disease.
Biosci Rep. 2019 06 28; 39(6)BR

Abstract

Kashin-beck disease (KBD) is endemic chronic osteoarthrosis and its pathogenesis is still unclear. The present study aimed to explore differential gene expression in articular cartilage between patients with rheumatoid arthritis (RA) and KBD. Articular cartilages were collected from KBD and RA patients, and differentially expressed genes (DEGs) were analyzed by RNA-seq. The signaling pathway and biological process (BP) of the DEGs were identified by enrichment analysis. The protein-protein interaction (PPI) network of DEGs and the key genes of KBD were identified by network analysis with STRING and cytoscape software. We identified 167 immune-related DEGs in articular cartilage samples from KBD patients compared with RA. The up-regulation of MAPK signaling pathway and the down-regulation of signaling pathways such as toll-like receptor, janus kinase-signal transducers and activators of transcription, leukocyte migration, T-cell receptor and chemokine, and antigen processing and presentation were involved in KBD. We identified 137 genes nodes related with immune and mapped the PPI network diagram. BP analysis revealed that immune response, calcium ion homeostasis, blood vessel morphogenesis, inflammatory response, lymphocyte proliferation, and MAPK activation were involved in KBD. In conclusion, gene expression profiling can be used to identify the different mechanism of pathogenesis between KBD and RA.

Authors+Show Affiliations

Orthopedic Department of The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.Institute of Endemic Diseases, School of Public Health of Health Science Center, Xi'an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an 710061, China duancmail@xjtu.edu.cn.Institute of Endemic Diseases, School of Public Health of Health Science Center, Xi'an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an 710061, China.Institute of Endemic Diseases, School of Public Health of Health Science Center, Xi'an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an 710061, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31196963

Citation

Gao, Zongqiang, et al. "Differential Gene Expression in Articular Cartilage Between Rheumatoid Arthritis and Endemic Kashin-Beck Disease." Bioscience Reports, vol. 39, no. 6, 2019.
Gao Z, Duan C, Yu FF, et al. Differential gene expression in articular cartilage between rheumatoid arthritis and endemic Kashin-Beck disease. Biosci Rep. 2019;39(6).
Gao, Z., Duan, C., Yu, F. F., & Guo, X. (2019). Differential gene expression in articular cartilage between rheumatoid arthritis and endemic Kashin-Beck disease. Bioscience Reports, 39(6). https://doi.org/10.1042/BSR20190188
Gao Z, et al. Differential Gene Expression in Articular Cartilage Between Rheumatoid Arthritis and Endemic Kashin-Beck Disease. Biosci Rep. 2019 06 28;39(6) PubMed PMID: 31196963.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential gene expression in articular cartilage between rheumatoid arthritis and endemic Kashin-Beck disease. AU - Gao,Zongqiang, AU - Duan,Chen, AU - Yu,Fang-Fang, AU - Guo,Xiong, Y1 - 2019/06/28/ PY - 2019/01/21/received PY - 2019/05/15/revised PY - 2019/06/11/accepted PY - 2019/6/15/pubmed PY - 2020/8/7/medline PY - 2019/6/15/entrez KW - Kashin-Beck disease KW - RNA-seq KW - Rheumatoid arthritis KW - gene expression KW - immune system JF - Bioscience reports JO - Biosci. Rep. VL - 39 IS - 6 N2 - Kashin-beck disease (KBD) is endemic chronic osteoarthrosis and its pathogenesis is still unclear. The present study aimed to explore differential gene expression in articular cartilage between patients with rheumatoid arthritis (RA) and KBD. Articular cartilages were collected from KBD and RA patients, and differentially expressed genes (DEGs) were analyzed by RNA-seq. The signaling pathway and biological process (BP) of the DEGs were identified by enrichment analysis. The protein-protein interaction (PPI) network of DEGs and the key genes of KBD were identified by network analysis with STRING and cytoscape software. We identified 167 immune-related DEGs in articular cartilage samples from KBD patients compared with RA. The up-regulation of MAPK signaling pathway and the down-regulation of signaling pathways such as toll-like receptor, janus kinase-signal transducers and activators of transcription, leukocyte migration, T-cell receptor and chemokine, and antigen processing and presentation were involved in KBD. We identified 137 genes nodes related with immune and mapped the PPI network diagram. BP analysis revealed that immune response, calcium ion homeostasis, blood vessel morphogenesis, inflammatory response, lymphocyte proliferation, and MAPK activation were involved in KBD. In conclusion, gene expression profiling can be used to identify the different mechanism of pathogenesis between KBD and RA. SN - 1573-4935 UR - https://www.unboundmedicine.com/medline/citation/31196963/Differential_gene_expression_in_articular_cartilage_between_rheumatoid_arthritis_and_endemic_Kashin-Beck_disease L2 - https://portlandpress.com/bioscirep/article-lookup/doi/10.1042/BSR20190188 DB - PRIME DP - Unbound Medicine ER -