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Corticosterone Upregulates Gene and Protein Expression of Catecholamine Markers in Organotypic Brainstem Cultures.

Abstract

Glucocorticoids are produced by the adrenal cortex and regulate cell metabolism in a variety of organs. This occurs either directly, by acting on specific receptors in a variety of cells, or by stimulating catecholamine expression within neighbor cells of the adrenal medulla. In this way, the whole adrenal gland may support specific metabolic requirements to cope with stressful conditions from external environment or internal organs. In addition, glucocorticoid levels may increase significantly in the presence of inappropriate secretion from adrenal cortex or may be administered at high doses to treat inflammatory disorders. In these conditions, metabolic alterations and increased blood pressure may occur, although altered sleep-waking cycle, anxiety, and mood disorders are frequent. These latter symptoms remain unexplained at the molecular level, although they overlap remarkably with disorders affecting catecholamine nuclei of the brainstem reticular formation. In fact, the present study indicates that various doses of glucocorticoids alter the expression of genes and proteins, which are specific for reticular catecholamine neurons. In detail, corticosterone administration to organotypic mouse brainstem cultures significantly increases Tyrosine hydroxylase (TH) and Dopamine transporter (DAT), while Phenylethanolamine N-methyltransferase (PNMT) is not affected. On the other hand, Dopamine Beta-Hydroxylase (DBH) increases only after very high doses of corticosterone.

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  • Authors+Show Affiliations

    ,

    I.R.C.C.S. Neuromed, 86077 Pozzilli, Italy. carla.busceti@neuromed.it.

    ,

    I.R.C.C.S. Neuromed, 86077 Pozzilli, Italy. rosangela.ferese@neuromed.it.

    ,

    I.R.C.C.S. Neuromed, 86077 Pozzilli, Italy. domenico.bucci@neuromed.it.

    ,

    Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56126 Pisa, Italy. larisa.ryskalin@unipi.it.

    ,

    I.R.C.C.S. Neuromed, 86077 Pozzilli, Italy. stefano.gambardella@neuromed.it.

    ,

    I.R.C.C.S. Neuromed, 86077 Pozzilli, Italy. stabulario@neuromed.it.

    ,

    I.R.C.C.S. Neuromed, 86077 Pozzilli, Italy. nicoletti@neuromed.it. Department of Physiology and Pharmacology, University Sapienza, 00185 Roma, Italy. nicoletti@neuromed.it.

    I.R.C.C.S. Neuromed, 86077 Pozzilli, Italy. francesco.fornai@neuromed.it. Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56126 Pisa, Italy. francesco.fornai@neuromed.it.

    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31197099

    Citation

    Busceti, Carla L., et al. "Corticosterone Upregulates Gene and Protein Expression of Catecholamine Markers in Organotypic Brainstem Cultures." International Journal of Molecular Sciences, vol. 20, no. 12, 2019.
    Busceti CL, Ferese R, Bucci D, et al. Corticosterone Upregulates Gene and Protein Expression of Catecholamine Markers in Organotypic Brainstem Cultures. Int J Mol Sci. 2019;20(12).
    Busceti, C. L., Ferese, R., Bucci, D., Ryskalin, L., Gambardella, S., Madonna, M., ... Fornai, F. (2019). Corticosterone Upregulates Gene and Protein Expression of Catecholamine Markers in Organotypic Brainstem Cultures. International Journal of Molecular Sciences, 20(12), doi:10.3390/ijms20122901.
    Busceti CL, et al. Corticosterone Upregulates Gene and Protein Expression of Catecholamine Markers in Organotypic Brainstem Cultures. Int J Mol Sci. 2019 Jun 14;20(12) PubMed PMID: 31197099.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Corticosterone Upregulates Gene and Protein Expression of Catecholamine Markers in Organotypic Brainstem Cultures. AU - Busceti,Carla L, AU - Ferese,Rosangela, AU - Bucci,Domenico, AU - Ryskalin,Larisa, AU - Gambardella,Stefano, AU - Madonna,Michele, AU - Nicoletti,Ferdinando, AU - Fornai,Francesco, Y1 - 2019/06/14/ PY - 2019/04/09/received PY - 2019/06/04/revised PY - 2019/06/12/accepted PY - 2019/6/15/entrez KW - dopamine KW - dopamine transporter KW - glucocorticoids KW - noradrenaline KW - reticular formation KW - tyrosine hydroxylase JF - International journal of molecular sciences JO - Int J Mol Sci VL - 20 IS - 12 N2 - Glucocorticoids are produced by the adrenal cortex and regulate cell metabolism in a variety of organs. This occurs either directly, by acting on specific receptors in a variety of cells, or by stimulating catecholamine expression within neighbor cells of the adrenal medulla. In this way, the whole adrenal gland may support specific metabolic requirements to cope with stressful conditions from external environment or internal organs. In addition, glucocorticoid levels may increase significantly in the presence of inappropriate secretion from adrenal cortex or may be administered at high doses to treat inflammatory disorders. In these conditions, metabolic alterations and increased blood pressure may occur, although altered sleep-waking cycle, anxiety, and mood disorders are frequent. These latter symptoms remain unexplained at the molecular level, although they overlap remarkably with disorders affecting catecholamine nuclei of the brainstem reticular formation. In fact, the present study indicates that various doses of glucocorticoids alter the expression of genes and proteins, which are specific for reticular catecholamine neurons. In detail, corticosterone administration to organotypic mouse brainstem cultures significantly increases Tyrosine hydroxylase (TH) and Dopamine transporter (DAT), while Phenylethanolamine N-methyltransferase (PNMT) is not affected. On the other hand, Dopamine Beta-Hydroxylase (DBH) increases only after very high doses of corticosterone. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/31197099/Corticosterone_Upregulates_Gene_and_Protein_Expression_of_Catecholamine_Markers_in_Organotypic_Brainstem_Cultures L2 - http://www.mdpi.com/resolver?pii=ijms20122901 DB - PRIME DP - Unbound Medicine ER -