Tags

Type your tag names separated by a space and hit enter

Regulation of the Serotonergic System by Kainate in the Avian Retina.
Cell Mol Neurobiol 2019; 39(7):1039-1049CM

Abstract

Serotonin (5-HT) has been recognized as a neurotransmitter in the vertebrate retina, restricted mainly to amacrine and bipolar cells. It is involved with synaptic processing and possibly as a mitogenic factor. We confirm that chick retina amacrine and bipolar cells are, respectively, heavily and faintly immunolabeled for 5-HT. Amacrine serotonergic cells also co-express tyrosine hydroxylase (TH), a marker of dopaminergic cells in the retina. Previous reports demonstrated that serotonin transport can be modulated by neurotransmitter receptor activation. As 5-HT is diffusely released as a neuromodulator and co-localized with other transmitters, we evaluated if 5-HT uptake or release is modulated by several mediators in the avian retina. The role of different glutamate receptors on serotonin transport and release in vitro and in vivo was also studied. We show that L-glutamate induces an inhibitory effect on [3H]5-HT uptake and this effect was specific to kainate receptor activation. Kainate-induced decrease in [3H]5-HT uptake was blocked by CNQX, an AMPA/kainate receptor antagonist, but not by MK-801, a NMDA receptor antagonist. [3H]5-HT uptake was not observed in the presence of AMPA, thus suggesting that the decrease in serotonin uptake is mediated by kainate. 5-HT (10-50 μM) had no intrinsic activity in raising intracellular Ca2+, but addition of 10 μM 5-HT decreased Ca2+ shifts induced by KCl in retinal neurons. Moreover, kainate decreased the number of bipolar and amacrine cells labeled to serotonin in chick retina. In conclusion, our data suggest a highly selective effect of kainate receptors in the regulation of serotonin functions in the retinal cells.

Authors+Show Affiliations

Laboratório de Neuroquímica Molecular e Celular, Instituto de Ciências Biológicas, Universidade Federal do Pará, Campus Universitário do Guamá, Rua Augusto Correa 01, Belém-PA, 66075-110, Brazil.Laboratório de Neuroquímica Molecular e Celular, Instituto de Ciências Biológicas, Universidade Federal do Pará, Campus Universitário do Guamá, Rua Augusto Correa 01, Belém-PA, 66075-110, Brazil.Laboratório de Neuroquímica Molecular e Celular, Instituto de Ciências Biológicas, Universidade Federal do Pará, Campus Universitário do Guamá, Rua Augusto Correa 01, Belém-PA, 66075-110, Brazil.Lab de Neurobiologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém-PA, Brazil.Lab de Neurobiologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém-PA, Brazil.Laboratório de Neuroquímica, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio De Janeiro-RJ, Brazil. Escola de Ciências da Saúde, Centro Universitário IBMR, Rio De Janeiro-RJ, Brazil.Laboratório de Neuroquímica, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio De Janeiro-RJ, Brazil.Laboratório de Neuroquímica, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio De Janeiro-RJ, Brazil.Lab Neurobiologia da Retina, Programa de Pós-graduação em Neurociências, Universidade Federal Fluminense, Rio De Janeiro-RJ, Brazil.Laboratório de Neuroquímica, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio De Janeiro-RJ, Brazil.Laboratório de Neuroquímica Molecular e Celular, Instituto de Ciências Biológicas, Universidade Federal do Pará, Campus Universitário do Guamá, Rua Augusto Correa 01, Belém-PA, 66075-110, Brazil. jlmn@ufpa.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31197744

Citation

Passos, Adelaide da Conceição Fonseca, et al. "Regulation of the Serotonergic System By Kainate in the Avian Retina." Cellular and Molecular Neurobiology, vol. 39, no. 7, 2019, pp. 1039-1049.
Passos ADCF, Herculano AM, Oliveira KRHM, et al. Regulation of the Serotonergic System by Kainate in the Avian Retina. Cell Mol Neurobiol. 2019;39(7):1039-1049.
Passos, A. D. C. F., Herculano, A. M., Oliveira, K. R. H. M., de Lima, S. M. A., Rocha, F. A. F., Freitas, H. R., ... do Nascimento, J. L. M. (2019). Regulation of the Serotonergic System by Kainate in the Avian Retina. Cellular and Molecular Neurobiology, 39(7), pp. 1039-1049. doi:10.1007/s10571-019-00701-8.
Passos ADCF, et al. Regulation of the Serotonergic System By Kainate in the Avian Retina. Cell Mol Neurobiol. 2019;39(7):1039-1049. PubMed PMID: 31197744.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of the Serotonergic System by Kainate in the Avian Retina. AU - Passos,Adelaide da Conceição Fonseca, AU - Herculano,Anderson Manoel, AU - Oliveira,Karen R H M, AU - de Lima,Silene Maria A, AU - Rocha,Fernando A F, AU - Freitas,Hércules Rezende, AU - da Silva Sampaio,Luzia, AU - Figueiredo,Danniel Pereira, AU - da Costa Calaza,Karin, AU - de Melo Reis,Ricardo Augusto, AU - do Nascimento,José Luiz Martins, Y1 - 2019/06/13/ PY - 2019/02/05/received PY - 2019/06/07/accepted PY - 2019/6/15/pubmed PY - 2019/6/15/medline PY - 2019/6/15/entrez KW - Glutamate receptors KW - Kainate KW - Retina KW - Serotonin SP - 1039 EP - 1049 JF - Cellular and molecular neurobiology JO - Cell. Mol. Neurobiol. VL - 39 IS - 7 N2 - Serotonin (5-HT) has been recognized as a neurotransmitter in the vertebrate retina, restricted mainly to amacrine and bipolar cells. It is involved with synaptic processing and possibly as a mitogenic factor. We confirm that chick retina amacrine and bipolar cells are, respectively, heavily and faintly immunolabeled for 5-HT. Amacrine serotonergic cells also co-express tyrosine hydroxylase (TH), a marker of dopaminergic cells in the retina. Previous reports demonstrated that serotonin transport can be modulated by neurotransmitter receptor activation. As 5-HT is diffusely released as a neuromodulator and co-localized with other transmitters, we evaluated if 5-HT uptake or release is modulated by several mediators in the avian retina. The role of different glutamate receptors on serotonin transport and release in vitro and in vivo was also studied. We show that L-glutamate induces an inhibitory effect on [3H]5-HT uptake and this effect was specific to kainate receptor activation. Kainate-induced decrease in [3H]5-HT uptake was blocked by CNQX, an AMPA/kainate receptor antagonist, but not by MK-801, a NMDA receptor antagonist. [3H]5-HT uptake was not observed in the presence of AMPA, thus suggesting that the decrease in serotonin uptake is mediated by kainate. 5-HT (10-50 μM) had no intrinsic activity in raising intracellular Ca2+, but addition of 10 μM 5-HT decreased Ca2+ shifts induced by KCl in retinal neurons. Moreover, kainate decreased the number of bipolar and amacrine cells labeled to serotonin in chick retina. In conclusion, our data suggest a highly selective effect of kainate receptors in the regulation of serotonin functions in the retinal cells. SN - 1573-6830 UR - https://www.unboundmedicine.com/medline/citation/31197744/Regulation_of_the_Serotonergic_System_by_Kainate_in_the_Avian_Retina L2 - https://doi.org/10.1007/s10571-019-00701-8 DB - PRIME DP - Unbound Medicine ER -