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Identification and expression of the 11β-steroid hydroxylase from Cochliobolus lunatus in Corynebacterium glutamicum.

Abstract

Hydroxylation of steroids has acquired special relevance for the pharmaceutical industries. Particularly, the 11β-hydroxylation of steroids is a reaction of biotechnological importance currently carried out at industrial scale by the fungus Cochliobolus lunatus. In this work, we have identified the genes encoding the cytochrome CYP103168 and the reductase CPR64795 of C. lunatus responsible for the 11β-hydroxylase activity in this fungus, which is the key step for the preparative synthesis of cortisol in industry. A recombinant Corynebacterium glutamicum strain harbouring a plasmid expressing both genes forming a synthetic bacterial operon was able to 11β-hydroxylate several steroids as substrates. This is a new example to show that the industrial strain C. glutamicum can be used as a suitable chassis to perform steroid biotransformation expressing eukaryotic cytochromes.

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  • Authors+Show Affiliations

    ,

    Department of Environmental Biology, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.

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    Department of Environmental Biology, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.

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    Department of Molecular Biology, University of León, León, Spain.

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    Department of Molecular Biology, University of León, León, Spain.

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    Department of Environmental Biology, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.

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    Department of Chemical and Physical Biology, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.

    Department of Environmental Biology, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain. Department of Applied Biotechnology, Institute for Integrative Systems Biology (I2SysBio), (Universidad de Valencia-CSIC), Valencia, Spain.

    Source

    Microbial biotechnology : 2019 Jun 14 pg

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31197939

    Citation

    Felpeto-Santero, Carmen, et al. "Identification and Expression of the 11β-steroid Hydroxylase From Cochliobolus Lunatus in Corynebacterium Glutamicum." Microbial Biotechnology, 2019.
    Felpeto-Santero C, Galán B, Luengo JM, et al. Identification and expression of the 11β-steroid hydroxylase from Cochliobolus lunatus in Corynebacterium glutamicum. Microb Biotechnol. 2019.
    Felpeto-Santero, C., Galán, B., Luengo, J. M., Fernández-Cañon, J. M., Del Cerro, C., Medrano, F. J., & García, J. L. (2019). Identification and expression of the 11β-steroid hydroxylase from Cochliobolus lunatus in Corynebacterium glutamicum. Microbial Biotechnology, doi:10.1111/1751-7915.13428.
    Felpeto-Santero C, et al. Identification and Expression of the 11β-steroid Hydroxylase From Cochliobolus Lunatus in Corynebacterium Glutamicum. Microb Biotechnol. 2019 Jun 14; PubMed PMID: 31197939.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Identification and expression of the 11β-steroid hydroxylase from Cochliobolus lunatus in Corynebacterium glutamicum. AU - Felpeto-Santero,Carmen, AU - Galán,Beatriz, AU - Luengo,José M, AU - Fernández-Cañon,José M, AU - Del Cerro,Carlos, AU - Medrano,Francisco J, AU - García,José L, Y1 - 2019/06/14/ PY - 2019/02/18/received PY - 2019/04/26/revised PY - 2019/04/30/accepted PY - 2019/6/15/entrez JF - Microbial biotechnology JO - Microb Biotechnol N2 - Hydroxylation of steroids has acquired special relevance for the pharmaceutical industries. Particularly, the 11β-hydroxylation of steroids is a reaction of biotechnological importance currently carried out at industrial scale by the fungus Cochliobolus lunatus. In this work, we have identified the genes encoding the cytochrome CYP103168 and the reductase CPR64795 of C. lunatus responsible for the 11β-hydroxylase activity in this fungus, which is the key step for the preparative synthesis of cortisol in industry. A recombinant Corynebacterium glutamicum strain harbouring a plasmid expressing both genes forming a synthetic bacterial operon was able to 11β-hydroxylate several steroids as substrates. This is a new example to show that the industrial strain C. glutamicum can be used as a suitable chassis to perform steroid biotransformation expressing eukaryotic cytochromes. SN - 1751-7915 UR - https://www.unboundmedicine.com/medline/citation/31197939/Identification_and_expression_of_the_11β-steroid_hydroxylase_from_Cochliobolus_lunatus_in_Corynebacterium_glutamicum L2 - https://doi.org/10.1111/1751-7915.13428 DB - PRIME DP - Unbound Medicine ER -