Tags

Type your tag names separated by a space and hit enter

The protective effect of Korean Red Ginseng against rotenone-induced Parkinson's disease in rat model: modulation of nuclear factor-κβ and caspase-3.

Abstract

OBJECTIVE

Korean red ginseng was reported to have many biological effects like the antioxidant and the anti-inflammatory activities. Oxidative stress and neuro-inflammation play major roles in the pathogenesis of Parkinson's disease (PD). The current study aimed to investigate the protective effects of ginseng on rotenone-induced PD in rats.

METHODS

Rats were randomly allocated into 4 groups: normal rats, rotenone control, ginseng+rotenone and ginseng only treated rats. The severity of PD was evaluated through locomotor activity perceiving in the open field test, histological examination and immunohistochemical detection of amyloid-β in brain tissues, in addition to the biochemical assessment of tyrosine hydroxylase activity in brain tissues. Moreover, the following parameters were investigated for studying the possible mechanisms of ginseng neuroprotective effect: nuclear factor-κβ (NF-κβ), tumor necrosis factor-alpha (TNF-α), caspase-3, lipid peroxides and reduced glutathione (GSH).

RESULTS

Ginseng exhibited potent neuroprotective effect that was reflected upon the histopathological examination, the marked improvement in the locomotor activity and through its ability to suppress the amyloid-β deposition in the cortex and striatum along with the significant increase in the tyrosine hydroxylase activity. Ginseng successfully inhibited the NF-κβ inflammatory pathway in brain tissues beside the inhibition of other oxidative stress and inflammatory mediators. Furthermore, it exhibited anti-apoptotic effect via the inhibition of caspase-3 expression.

CONCLUSION

Ginseng could be a promising treatment in PD. It can suppress the dopaminergic neuron degeneration through variable mechanisms mainly via inhibition of NF-κβ pathway in addition to inhibition oxidative stress and apoptosis.

Links

  • Aggregator Full Text
  • Authors+Show Affiliations

    ,

    Pharmacology & Toxicology Department, Faculty of Pharmacy, MSA University. Egypt.

    ,

    Pharmacology & Toxicology Department, Faculty of Pharmacy, MSA University. Egypt.

    Biochemistry Department, Faculty of Pharmacy, MSA University. Egypt.

    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31198107

    Citation

    Zaafan, Mai A., et al. "The Protective Effect of Korean Red Ginseng Against Rotenone-induced Parkinson's Disease in Rat Model: Modulation of Nuclear Factor-κβ and Caspase-3." Current Pharmaceutical Biotechnology, 2019.
    Zaafan MA, Abdelhamid AM, Ibrahim SM. The protective effect of Korean Red Ginseng against rotenone-induced Parkinson's disease in rat model: modulation of nuclear factor-κβ and caspase-3. Curr Pharm Biotechnol. 2019.
    Zaafan, M. A., Abdelhamid, A. M., & Ibrahim, S. M. (2019). The protective effect of Korean Red Ginseng against rotenone-induced Parkinson's disease in rat model: modulation of nuclear factor-κβ and caspase-3. Current Pharmaceutical Biotechnology, doi:10.2174/1389201020666190611122747.
    Zaafan MA, Abdelhamid AM, Ibrahim SM. The Protective Effect of Korean Red Ginseng Against Rotenone-induced Parkinson's Disease in Rat Model: Modulation of Nuclear Factor-κβ and Caspase-3. Curr Pharm Biotechnol. 2019 Jun 11; PubMed PMID: 31198107.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - The protective effect of Korean Red Ginseng against rotenone-induced Parkinson's disease in rat model: modulation of nuclear factor-κβ and caspase-3. AU - Zaafan,Mai A, AU - Abdelhamid,Amr M, AU - Ibrahim,Sherine M, Y1 - 2019/06/11/ PY - 2019/11/05/received PY - 2019/03/06/revised PY - 2019/04/29/accepted PY - 2019/6/15/entrez KW - Ginseng KW - Parkinson’s disease KW - anti-oxidant KW - caspase-3 KW - nuclear factor-κβ JF - Current pharmaceutical biotechnology JO - Curr Pharm Biotechnol N2 - OBJECTIVE: Korean red ginseng was reported to have many biological effects like the antioxidant and the anti-inflammatory activities. Oxidative stress and neuro-inflammation play major roles in the pathogenesis of Parkinson's disease (PD). The current study aimed to investigate the protective effects of ginseng on rotenone-induced PD in rats. METHODS: Rats were randomly allocated into 4 groups: normal rats, rotenone control, ginseng+rotenone and ginseng only treated rats. The severity of PD was evaluated through locomotor activity perceiving in the open field test, histological examination and immunohistochemical detection of amyloid-β in brain tissues, in addition to the biochemical assessment of tyrosine hydroxylase activity in brain tissues. Moreover, the following parameters were investigated for studying the possible mechanisms of ginseng neuroprotective effect: nuclear factor-κβ (NF-κβ), tumor necrosis factor-alpha (TNF-α), caspase-3, lipid peroxides and reduced glutathione (GSH). RESULTS: Ginseng exhibited potent neuroprotective effect that was reflected upon the histopathological examination, the marked improvement in the locomotor activity and through its ability to suppress the amyloid-β deposition in the cortex and striatum along with the significant increase in the tyrosine hydroxylase activity. Ginseng successfully inhibited the NF-κβ inflammatory pathway in brain tissues beside the inhibition of other oxidative stress and inflammatory mediators. Furthermore, it exhibited anti-apoptotic effect via the inhibition of caspase-3 expression. CONCLUSION: Ginseng could be a promising treatment in PD. It can suppress the dopaminergic neuron degeneration through variable mechanisms mainly via inhibition of NF-κβ pathway in addition to inhibition oxidative stress and apoptosis. SN - 1873-4316 UR - https://www.unboundmedicine.com/medline/citation/31198107/The_protective_effect_of_Korean_Red_Ginseng_against_rotenone-induced_Parkinson's_disease_in_rat_model:_modulation_of_nuclear_factor-κβ_and_caspase-3 L2 - https://www.ingentaconnect.com/content/10.2174/1389201020666190611122747 DB - PRIME DP - Unbound Medicine ER -