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Initiation events in in-vitro packaging of bacteriophage phi 29 DNA-gp3.
J Mol Biol 1987; 197(2):219-28JM

Abstract

Initiation events in the packaging of bacteriophage phi 29 DNA-gp3 (DNA-gene product 3 complex) were studied in a completely defined in-vitro system that included purified proheads, DNA-gp3 and the DNA packaging protein gp16. In the sequential interactions, gp16 first bound to, and was modified by, the prohead. The prohead-gp16 complex then bound to DNA-gp3, resulting in a second modification of gp16 that permitted binding of ATP. DNA-gp3 aggregates were produced, and the hydrolysis of ATP accompanied DNA-gp3 packaging. Binding and hydrolysis of ATP by gp16 was both prohead- and DNA-gp3-dependent. Interruption of packaging by DNase I addition revealed filled heads but few particles containing partial lengths of DNA, suggesting that following a rate-limiting initiation, the translocation of DNA-gp3 into the prohead was much faster in the defined in-vitro system than in extracts.

Authors+Show Affiliations

Department of Microbiology, University of Minnesota, Minneapolis 55455.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3119862

Citation

Guo, P, et al. "Initiation Events in In-vitro Packaging of Bacteriophage Phi 29 DNA-gp3." Journal of Molecular Biology, vol. 197, no. 2, 1987, pp. 219-28.
Guo P, Peterson C, Anderson D. Initiation events in in-vitro packaging of bacteriophage phi 29 DNA-gp3. J Mol Biol. 1987;197(2):219-28.
Guo, P., Peterson, C., & Anderson, D. (1987). Initiation events in in-vitro packaging of bacteriophage phi 29 DNA-gp3. Journal of Molecular Biology, 197(2), pp. 219-28.
Guo P, Peterson C, Anderson D. Initiation Events in In-vitro Packaging of Bacteriophage Phi 29 DNA-gp3. J Mol Biol. 1987 Sep 20;197(2):219-28. PubMed PMID: 3119862.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Initiation events in in-vitro packaging of bacteriophage phi 29 DNA-gp3. AU - Guo,P, AU - Peterson,C, AU - Anderson,D, PY - 1987/9/20/pubmed PY - 1987/9/20/medline PY - 1987/9/20/entrez SP - 219 EP - 28 JF - Journal of molecular biology JO - J. Mol. Biol. VL - 197 IS - 2 N2 - Initiation events in the packaging of bacteriophage phi 29 DNA-gp3 (DNA-gene product 3 complex) were studied in a completely defined in-vitro system that included purified proheads, DNA-gp3 and the DNA packaging protein gp16. In the sequential interactions, gp16 first bound to, and was modified by, the prohead. The prohead-gp16 complex then bound to DNA-gp3, resulting in a second modification of gp16 that permitted binding of ATP. DNA-gp3 aggregates were produced, and the hydrolysis of ATP accompanied DNA-gp3 packaging. Binding and hydrolysis of ATP by gp16 was both prohead- and DNA-gp3-dependent. Interruption of packaging by DNase I addition revealed filled heads but few particles containing partial lengths of DNA, suggesting that following a rate-limiting initiation, the translocation of DNA-gp3 into the prohead was much faster in the defined in-vitro system than in extracts. SN - 0022-2836 UR - https://www.unboundmedicine.com/medline/citation/3119862/Initiation_events_in_in_vitro_packaging_of_bacteriophage_phi_29_DNA_gp3_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0022-2836(87)90120-3 DB - PRIME DP - Unbound Medicine ER -