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A time domain study of surfactin penetrating a phospholipid monolayer at the air-water interface investigated using sum frequency generation spectroscopy, infrared reflection absorption spectroscopy, and AFM-nano infrared microscopy.
Biochim Biophys Acta Biomembr 2019; 1861(9):1568-1578BB

Abstract

We have investigated the interaction of surfactin with a monolayer of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) at the air-water interface as a function of time, following its injection into the sub-phase, using non-linear Sum Frequency Generation (SFG) vibrational spectroscopy and Infrared Reflection Absorption Spectroscopy (IRRAS). SFG resonances from the phospholipid and from the surfactin were distinguished from each other by using selective deuteration. The surface pressure at the interface was measured concurrently for up to 8 h. After an induction period, the spectra from the lipid diminished and those of surfactin gradually appeared whilst at the same time the surface pressure increased. However, eventually the surfactin signals disappeared and those of the lipid reappeared. Although the SFG spectra of the lipid disappeared at intermediate times, the IRRAS spectra of the lipid were always present at the interface. Variation in the temporal SFG behaviour was investigated as the pH of the sub-phase, the initial surface pressure of the lipid, and the surfactin concentration were changed. Samples of the surface film were transferred onto mica substrates at selected times along the temporal profile and imaged by Atomic Force Microscopy - nano Infrared Spectroscopy (nano-IR). A model is proposed to account for the results from the four different experimental techniques used.

Authors+Show Affiliations

The Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.The Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.The Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.The Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK. Electronic address: pbd2@cam.ac.uk.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31199896

Citation

Goussous, S A., et al. "A Time Domain Study of Surfactin Penetrating a Phospholipid Monolayer at the Air-water Interface Investigated Using Sum Frequency Generation Spectroscopy, Infrared Reflection Absorption Spectroscopy, and AFM-nano Infrared Microscopy." Biochimica Et Biophysica Acta. Biomembranes, vol. 1861, no. 9, 2019, pp. 1568-1578.
Goussous SA, Fellows AP, Casford MTL, et al. A time domain study of surfactin penetrating a phospholipid monolayer at the air-water interface investigated using sum frequency generation spectroscopy, infrared reflection absorption spectroscopy, and AFM-nano infrared microscopy. Biochim Biophys Acta Biomembr. 2019;1861(9):1568-1578.
Goussous, S. A., Fellows, A. P., Casford, M. T. L., & Davies, P. B. (2019). A time domain study of surfactin penetrating a phospholipid monolayer at the air-water interface investigated using sum frequency generation spectroscopy, infrared reflection absorption spectroscopy, and AFM-nano infrared microscopy. Biochimica Et Biophysica Acta. Biomembranes, 1861(9), pp. 1568-1578. doi:10.1016/j.bbamem.2019.06.004.
Goussous SA, et al. A Time Domain Study of Surfactin Penetrating a Phospholipid Monolayer at the Air-water Interface Investigated Using Sum Frequency Generation Spectroscopy, Infrared Reflection Absorption Spectroscopy, and AFM-nano Infrared Microscopy. Biochim Biophys Acta Biomembr. 2019 Sep 1;1861(9):1568-1578. PubMed PMID: 31199896.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A time domain study of surfactin penetrating a phospholipid monolayer at the air-water interface investigated using sum frequency generation spectroscopy, infrared reflection absorption spectroscopy, and AFM-nano infrared microscopy. AU - Goussous,S A, AU - Fellows,A P, AU - Casford,M T L, AU - Davies,P B, Y1 - 2019/06/12/ PY - 2018/12/05/received PY - 2019/05/13/revised PY - 2019/06/07/accepted PY - 2019/6/15/pubmed PY - 2019/6/15/medline PY - 2019/6/15/entrez KW - Lipid monolayers KW - Nano infrared microscopy KW - Surfactin lipid interaction KW - Time domain SFG spectroscopy SP - 1568 EP - 1578 JF - Biochimica et biophysica acta. Biomembranes JO - Biochim Biophys Acta Biomembr VL - 1861 IS - 9 N2 - We have investigated the interaction of surfactin with a monolayer of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) at the air-water interface as a function of time, following its injection into the sub-phase, using non-linear Sum Frequency Generation (SFG) vibrational spectroscopy and Infrared Reflection Absorption Spectroscopy (IRRAS). SFG resonances from the phospholipid and from the surfactin were distinguished from each other by using selective deuteration. The surface pressure at the interface was measured concurrently for up to 8 h. After an induction period, the spectra from the lipid diminished and those of surfactin gradually appeared whilst at the same time the surface pressure increased. However, eventually the surfactin signals disappeared and those of the lipid reappeared. Although the SFG spectra of the lipid disappeared at intermediate times, the IRRAS spectra of the lipid were always present at the interface. Variation in the temporal SFG behaviour was investigated as the pH of the sub-phase, the initial surface pressure of the lipid, and the surfactin concentration were changed. Samples of the surface film were transferred onto mica substrates at selected times along the temporal profile and imaged by Atomic Force Microscopy - nano Infrared Spectroscopy (nano-IR). A model is proposed to account for the results from the four different experimental techniques used. SN - 1879-2642 UR - https://www.unboundmedicine.com/medline/citation/31199896/A_time_domain_study_of_surfactin_penetrating_a_phospholipid_monolayer_at_the_air-water_Interface_investigated_using_sum_frequency_generation_spectroscopy,_infrared_reflection_absorption_spectroscopy,_and_AFM-nano_infrared_microscopy L2 - https://linkinghub.elsevier.com/retrieve/pii/S0005-2736(19)30136-1 DB - PRIME DP - Unbound Medicine ER -