Tags

Type your tag names separated by a space and hit enter

Downregulation of microRNA-302b-3p relieves oxygen-glucose deprivation/re-oxygenation induced injury in murine hippocampal neurons through up-regulating Nrf2 signaling by targeting fibroblast growth factor 15/19.
Chem Biol Interact. 2019 Aug 25; 309:108705.CB

Abstract

MicroRNAs have emerged as critical mediators of cerebral ischaemia/reperfusion injury. Recent studies have demonstrated that microRNA-302b-3p (miR-302b-3p) plays an important role in regulating apoptosis and oxidative stress in various cells. However, whether miR-302b-3p is involved in regulating cerebral ischaemia/reperfusion injury-induced neuronal apoptosis and oxidative stress remains unknown. In the present study, we explored the potential function and molecular mechanism of miR-302b-3p in oxygen-glucose deprivation/re-oxygenation (OGD/R)-induced neuronal injury, using an in vitro model of cerebral ischaemia/reperfusion injury. We found that miR-302b-3p expression was up-regulated by OGD/R treatment in neurons. The inhibition of miR-302b-3p improved cell viability, and reduced apoptosis and the production of reactive oxygen species, showing a protective effect against OGD/R-induced injury. Interestingly, miR-302b-3p was shown to target and modulate murine fibroblast growth factor 15 (FGF15). Moreover, our results showed that miR-302b-3p down-regulation contributed to the promotion of nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE)-mediated antioxidant signaling associated with the inactivation of glycogen synthase kinase-3β. However, the knockdown of FGF15 significantly reversed the miR-302b-3p inhibition-mediated protective effect in OGD/R-treated neurons. Overall, these results demonstrated that miR-302b-3p inhibition confers a neuroprotective effect in OGD/R-treated neurons by up-regulating Nrf2/ARE antioxidant signaling via targeting FGF15, providing a novel target for neuroprotection in cerebral ischaemia/reperfusion injury.

Authors+Show Affiliations

Anesthesia Department, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.Anesthesia Department, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.Anesthesia Department, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.Anesthesia Department, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.Anesthesia Department, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, China. Electronic address: lvjianruiljr@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31199929

Citation

Zhang, Zhenni, et al. "Downregulation of microRNA-302b-3p Relieves Oxygen-glucose Deprivation/re-oxygenation Induced Injury in Murine Hippocampal Neurons Through Up-regulating Nrf2 Signaling By Targeting Fibroblast Growth Factor 15/19." Chemico-biological Interactions, vol. 309, 2019, p. 108705.
Zhang Z, Wang N, Zhang Y, et al. Downregulation of microRNA-302b-3p relieves oxygen-glucose deprivation/re-oxygenation induced injury in murine hippocampal neurons through up-regulating Nrf2 signaling by targeting fibroblast growth factor 15/19. Chem Biol Interact. 2019;309:108705.
Zhang, Z., Wang, N., Zhang, Y., Zhao, J., & Lv, J. (2019). Downregulation of microRNA-302b-3p relieves oxygen-glucose deprivation/re-oxygenation induced injury in murine hippocampal neurons through up-regulating Nrf2 signaling by targeting fibroblast growth factor 15/19. Chemico-biological Interactions, 309, 108705. https://doi.org/10.1016/j.cbi.2019.06.018
Zhang Z, et al. Downregulation of microRNA-302b-3p Relieves Oxygen-glucose Deprivation/re-oxygenation Induced Injury in Murine Hippocampal Neurons Through Up-regulating Nrf2 Signaling By Targeting Fibroblast Growth Factor 15/19. Chem Biol Interact. 2019 Aug 25;309:108705. PubMed PMID: 31199929.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Downregulation of microRNA-302b-3p relieves oxygen-glucose deprivation/re-oxygenation induced injury in murine hippocampal neurons through up-regulating Nrf2 signaling by targeting fibroblast growth factor 15/19. AU - Zhang,Zhenni, AU - Wang,Ning, AU - Zhang,Yong, AU - Zhao,Jing, AU - Lv,Jianrui, Y1 - 2019/06/11/ PY - 2019/04/12/received PY - 2019/05/30/revised PY - 2019/06/10/accepted PY - 2019/6/15/pubmed PY - 2019/8/20/medline PY - 2019/6/15/entrez KW - Cerebral ischaemia/reperfusion injury KW - FGF15 KW - GSK-3β KW - Nrf2 SP - 108705 EP - 108705 JF - Chemico-biological interactions JO - Chem. Biol. Interact. VL - 309 N2 - MicroRNAs have emerged as critical mediators of cerebral ischaemia/reperfusion injury. Recent studies have demonstrated that microRNA-302b-3p (miR-302b-3p) plays an important role in regulating apoptosis and oxidative stress in various cells. However, whether miR-302b-3p is involved in regulating cerebral ischaemia/reperfusion injury-induced neuronal apoptosis and oxidative stress remains unknown. In the present study, we explored the potential function and molecular mechanism of miR-302b-3p in oxygen-glucose deprivation/re-oxygenation (OGD/R)-induced neuronal injury, using an in vitro model of cerebral ischaemia/reperfusion injury. We found that miR-302b-3p expression was up-regulated by OGD/R treatment in neurons. The inhibition of miR-302b-3p improved cell viability, and reduced apoptosis and the production of reactive oxygen species, showing a protective effect against OGD/R-induced injury. Interestingly, miR-302b-3p was shown to target and modulate murine fibroblast growth factor 15 (FGF15). Moreover, our results showed that miR-302b-3p down-regulation contributed to the promotion of nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE)-mediated antioxidant signaling associated with the inactivation of glycogen synthase kinase-3β. However, the knockdown of FGF15 significantly reversed the miR-302b-3p inhibition-mediated protective effect in OGD/R-treated neurons. Overall, these results demonstrated that miR-302b-3p inhibition confers a neuroprotective effect in OGD/R-treated neurons by up-regulating Nrf2/ARE antioxidant signaling via targeting FGF15, providing a novel target for neuroprotection in cerebral ischaemia/reperfusion injury. SN - 1872-7786 UR - https://www.unboundmedicine.com/medline/citation/31199929/Downregulation_of_microRNA_302b_3p_relieves_oxygen_glucose_deprivation/re_oxygenation_induced_injury_in_murine_hippocampal_neurons_through_up_regulating_Nrf2_signaling_by_targeting_fibroblast_growth_factor_15/19_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-2797(19)30619-2 DB - PRIME DP - Unbound Medicine ER -