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Long non-coding RNA and MicroRNA profiling provides comprehensive insight into non-coding RNA involved host immune responses in ALV-J-infected chicken primary macrophage.

Abstract

Avian leukosis virus subgroup J (ALV-J) infection can cause tumors and immunosuppression in infected chickens. Macrophages play a crucial role in host defense against invading pathogens. In the present study, whole transcriptome analysis was performed to analyze the host factors including genes, microRNA (miRNA), long non-coding RNA (lncRNA) and their regulatory network in chicken primary monocyte-derived macrophages (MDMs). In total, 128 differentially expressed (DE) lncRNAs and 15 DE miRNAs were identified in MDMs at 3 h post infection (hpi), and 30 DE lncRNAs and 8 DE miRNAs were identified in MDMs at 36 hpi during ALV-J infection. We further constructed the DE lncRNAs-mRNAs, miRNA-mRNAs and lncRNAs-miRNA-mRNAs interaction networks. The results suggested that DE lncRNAs and miRNAs are involved in the regulation of CCND3 and SOCS5 in Jak-STAT signaling pathway via ceRNA network in ALV-J-infected MDMs at 3 hpi. In addition, lncRNAs including XLOC_672329, ALDBGALG0000001429, XLOC_016500 and ALDBGALG0000000253 cis-regulating CH25H, CISH, IL-1β and CD80 respectively in MDMs at 3 hpi participated in host antiviral responses. Our findings give a comprehensive view of the connection between non-coding RNA and ALV-J in chicken primary macrophages, and provide an excellent resource for further studies of epigenetic effects on ALV-J disease resistance breeding as well as immune system and genomic researches.

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  • Authors+Show Affiliations

    ,

    College of Veterinary Medicine, South China Agricultural University, Guangzhou, People's Republic of China. Electronic address: daimanman1229@163.com.

    ,

    Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China. Electronic address: hunanfengmin@163.com.

    ,

    Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China; Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, 510642, Guangdong, China. Electronic address: 1084256498@qq.com.

    Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China; Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, 510642, Guangdong, China. Electronic address: xqzhang@scau.edu.cn.

    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31200006

    Citation

    Dai, Manman, et al. "Long Non-coding RNA and MicroRNA Profiling Provides Comprehensive Insight Into Non-coding RNA Involved Host Immune Responses in ALV-J-infected Chicken Primary Macrophage." Developmental and Comparative Immunology, vol. 100, 2019, p. 103414.
    Dai M, Feng M, Xie T, et al. Long non-coding RNA and MicroRNA profiling provides comprehensive insight into non-coding RNA involved host immune responses in ALV-J-infected chicken primary macrophage. Dev Comp Immunol. 2019;100:103414.
    Dai, M., Feng, M., Xie, T., & Zhang, X. (2019). Long non-coding RNA and MicroRNA profiling provides comprehensive insight into non-coding RNA involved host immune responses in ALV-J-infected chicken primary macrophage. Developmental and Comparative Immunology, 100, p. 103414. doi:10.1016/j.dci.2019.103414.
    Dai M, et al. Long Non-coding RNA and MicroRNA Profiling Provides Comprehensive Insight Into Non-coding RNA Involved Host Immune Responses in ALV-J-infected Chicken Primary Macrophage. Dev Comp Immunol. 2019;100:103414. PubMed PMID: 31200006.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Long non-coding RNA and MicroRNA profiling provides comprehensive insight into non-coding RNA involved host immune responses in ALV-J-infected chicken primary macrophage. AU - Dai,Manman, AU - Feng,Min, AU - Xie,Tingting, AU - Zhang,Xiquan, Y1 - 2019/06/11/ PY - 2019/04/22/received PY - 2019/06/10/revised PY - 2019/06/10/accepted PY - 2019/6/15/pubmed PY - 2019/6/15/medline PY - 2019/6/15/entrez KW - ALV-J KW - Immune response KW - Macrophage KW - lncRNA KW - miRNA SP - 103414 EP - 103414 JF - Developmental and comparative immunology JO - Dev. Comp. Immunol. VL - 100 N2 - Avian leukosis virus subgroup J (ALV-J) infection can cause tumors and immunosuppression in infected chickens. Macrophages play a crucial role in host defense against invading pathogens. In the present study, whole transcriptome analysis was performed to analyze the host factors including genes, microRNA (miRNA), long non-coding RNA (lncRNA) and their regulatory network in chicken primary monocyte-derived macrophages (MDMs). In total, 128 differentially expressed (DE) lncRNAs and 15 DE miRNAs were identified in MDMs at 3 h post infection (hpi), and 30 DE lncRNAs and 8 DE miRNAs were identified in MDMs at 36 hpi during ALV-J infection. We further constructed the DE lncRNAs-mRNAs, miRNA-mRNAs and lncRNAs-miRNA-mRNAs interaction networks. The results suggested that DE lncRNAs and miRNAs are involved in the regulation of CCND3 and SOCS5 in Jak-STAT signaling pathway via ceRNA network in ALV-J-infected MDMs at 3 hpi. In addition, lncRNAs including XLOC_672329, ALDBGALG0000001429, XLOC_016500 and ALDBGALG0000000253 cis-regulating CH25H, CISH, IL-1β and CD80 respectively in MDMs at 3 hpi participated in host antiviral responses. Our findings give a comprehensive view of the connection between non-coding RNA and ALV-J in chicken primary macrophages, and provide an excellent resource for further studies of epigenetic effects on ALV-J disease resistance breeding as well as immune system and genomic researches. SN - 1879-0089 UR - https://www.unboundmedicine.com/medline/citation/31200006/Long_non-coding_RNA_and_MicroRNA_profiling_provides_comprehensive_insight_into_non-coding_RNA_involved_host_immune_responses_in_ALV-J-infected_chicken_primary_macrophage L2 - https://linkinghub.elsevier.com/retrieve/pii/S0145-305X(19)30192-2 DB - PRIME DP - Unbound Medicine ER -