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Propofol Relaxes Isolated Rat Aorta through BKCa Activation.

Abstract

BACKGROUND

Propofol is an intravenous anesthetic that can be used for the induction and maintenance of anesthesia. In the present study, it was aimed to investigate the mechanism of vasodilator action of propofol in the rat aorta (RA).

METHODS

The RA rings were suspended in isolated organ baths and tension was recorded isometrically. First, potassium chloride (KCl) and phenylephrine (PE) were added to organ baths to form precontraction. When the precontractions were stable, propofol (1, 10, and 100 μM) was added cumulatively to the baths. The antagonistic effect of propofol on KCl (45 mM), PE (1 μM), 5-hydroxytryptamine (5-HT) (30 μM), and calcium chloride (CaCl2) (10 μM to 10 mM) induced contractions in the vascular rings were investigated. Propofol-induced relaxations were also tested in the presence of the K+ channel inhibitors tetraethylammonium (TEA, 1 mM), glibenclamide (GLI, 10 μM), 4-aminopyridine (4-AP, 1 mM), and barium chloride (BaCl2, 30 μM).

RESULTS

Preincubation with propofol (1, 10, and 100 μM) did not affect the basal tone but inhibited the contraction induced by KCl, PE, 5-HT, and CaCl2-induced contractions. Propofol-induced relaxation was not effected by 4-AP, GLI, and BaCl2. However, TEA inhibited propofol-induced relaxations significantly.

CONCLUSIONS

The propofol induces relaxation in contracted RA and inhibits KCl, PE, 5-HT, and CaCl2-induced contractions. The results demonstrate that the mechanism of action of propofol-induced vasodilation in the RA may be related to large conductance Ca2+-activated K+ channel activation.

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  • Authors+Show Affiliations

    ,

    Department of Medical Pharmacology, Gulhane Faculty of Medicine, University of Health Sciences, Ankara, Turkey.

    ,

    Department of Medical Pharmacology, Faculty of Medicine, Ankara Yıldırım Beyazıt University, Ankara, Turkey.

    ,

    Department of Medical Pharmacology, Faculty of Medicine, Ankara Yıldırım Beyazıt University, Ankara, Turkey.

    ,

    Department of Medical Pharmacology, Faculty of Medicine, Ankara Yıldırım Beyazıt University, Ankara, Turkey.

    Department of Medical Pharmacology, Gulhane Faculty of Medicine, University of Health Sciences, Ankara, Turkey. Electronic address: oguzhan.yildiz@sbu.edu.tr.

    Source

    Annals of vascular surgery : 2019 Jun 12 pg

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31200052

    Citation

    Ulusoy, Kemal Gokhan, et al. "Propofol Relaxes Isolated Rat Aorta Through BKCa Activation." Annals of Vascular Surgery, 2019.
    Ulusoy KG, Dogan MF, Cam SA, et al. Propofol Relaxes Isolated Rat Aorta through BKCa Activation. Ann Vasc Surg. 2019.
    Ulusoy, K. G., Dogan, M. F., Cam, S. A., Arslan, S. O., & Yildiz, O. (2019). Propofol Relaxes Isolated Rat Aorta through BKCa Activation. Annals of Vascular Surgery, doi:10.1016/j.avsg.2019.04.005.
    Ulusoy KG, et al. Propofol Relaxes Isolated Rat Aorta Through BKCa Activation. Ann Vasc Surg. 2019 Jun 12; PubMed PMID: 31200052.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Propofol Relaxes Isolated Rat Aorta through BKCa Activation. AU - Ulusoy,Kemal Gokhan, AU - Dogan,Muhammed Fatih, AU - Cam,Saliha Aysenur, AU - Arslan,Seyfullah Oktay, AU - Yildiz,Oguzhan, Y1 - 2019/06/12/ PY - 2019/02/13/received PY - 2019/04/12/revised PY - 2019/04/13/accepted PY - 2019/6/15/pubmed PY - 2019/6/15/medline PY - 2019/6/15/entrez JF - Annals of vascular surgery JO - Ann Vasc Surg N2 - BACKGROUND: Propofol is an intravenous anesthetic that can be used for the induction and maintenance of anesthesia. In the present study, it was aimed to investigate the mechanism of vasodilator action of propofol in the rat aorta (RA). METHODS: The RA rings were suspended in isolated organ baths and tension was recorded isometrically. First, potassium chloride (KCl) and phenylephrine (PE) were added to organ baths to form precontraction. When the precontractions were stable, propofol (1, 10, and 100 μM) was added cumulatively to the baths. The antagonistic effect of propofol on KCl (45 mM), PE (1 μM), 5-hydroxytryptamine (5-HT) (30 μM), and calcium chloride (CaCl2) (10 μM to 10 mM) induced contractions in the vascular rings were investigated. Propofol-induced relaxations were also tested in the presence of the K+ channel inhibitors tetraethylammonium (TEA, 1 mM), glibenclamide (GLI, 10 μM), 4-aminopyridine (4-AP, 1 mM), and barium chloride (BaCl2, 30 μM). RESULTS: Preincubation with propofol (1, 10, and 100 μM) did not affect the basal tone but inhibited the contraction induced by KCl, PE, 5-HT, and CaCl2-induced contractions. Propofol-induced relaxation was not effected by 4-AP, GLI, and BaCl2. However, TEA inhibited propofol-induced relaxations significantly. CONCLUSIONS: The propofol induces relaxation in contracted RA and inhibits KCl, PE, 5-HT, and CaCl2-induced contractions. The results demonstrate that the mechanism of action of propofol-induced vasodilation in the RA may be related to large conductance Ca2+-activated K+ channel activation. SN - 1615-5947 UR - https://www.unboundmedicine.com/medline/citation/31200052/Propofol_relaxes_isolated_rat_aorta_through_BKCa_activation L2 - https://linkinghub.elsevier.com/retrieve/pii/S0890-5096(19)30386-3 DB - PRIME DP - Unbound Medicine ER -