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Promiscuous T cell epitopes boosts specific IgM immune response against a P0 peptide antigen from sea lice in different teleost species.

Abstract

The development of vaccines employing conserved protein antigens, for instance ribosomal protein P0, has as disadvantage the high degree of identity between pathogen and host proteins due to possible induction of tolerance or auto antibodies in the host organism. To overcome this drawback, peptide-based vaccines have been designed with a proved high efficacy. The use of defined peptides as antigens has the problem that they are generally poor immunogenic unless coupled to a carrier protein. Several studies have established the potential for promiscuous T cell epitopes incorporated into chimeric peptides to enhance the immunogenicity in mammals. On the contrary, studies about the role of these epitopes on teleost immune system are scarce. Therefore, the main objective of our present study was to evaluate the potential of promiscuous T cell epitopes to boost specific IgM immune response in teleost fish against a peptide antigen. With this aim, we used a peptide of 35 amino acids from the ribosomal P0 protein of Lepeophtheirus salmonis, an important parasite in salmon aquaculture. We fused this peptide to the C-terminal of T cell epitopes from tetanus toxin and measles virus and produced the chimeric protein in Escherichia coli. Following vaccination, IgM antibody production was monitored in different immunization schemes in Tilapia, African catfish and Atlantic salmon. The results demonstrated for first time that the addition of T cell epitopes at the N-terminal of a target peptide increased IgM specific response in different teleost species, revealing the potential of this approach to develop peptide-based vaccines for aquaculture. The results are also of great importance in the context of vaccine development against sea lice using ribosomal protein P0 as antigen taking into account the key role of P0 in protein synthesis and other essential physiological processes.

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  • Authors+Show Affiliations

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    Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba.

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    Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba.

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    Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba.

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    NOFIMA, Tromso, Norway; Fish Immunology and Vaccinology Research Group, Norwegian College of Fishery Science, UiT the Arctic University of Norway, Tromso, Norway.

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    Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba.

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    Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba.

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    Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba.

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    Physico-Chemistry Department, CIGB, Havana, Cuba.

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    Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba. Electronic address: mario.pablo@cigb.edu.cu.

    Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba. Electronic address: yamila.carpio@cigb.edu.cu.

    Source

    Fish & shellfish immunology 92: 2019 Jun 11 pg 322-330

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31200071

    Citation

    Leal, Yeny, et al. "Promiscuous T Cell Epitopes Boosts Specific IgM Immune Response Against a P0 Peptide Antigen From Sea Lice in Different Teleost Species." Fish & Shellfish Immunology, vol. 92, 2019, pp. 322-330.
    Leal Y, Velazquez J, Hernandez L, et al. Promiscuous T cell epitopes boosts specific IgM immune response against a P0 peptide antigen from sea lice in different teleost species. Fish Shellfish Immunol. 2019;92:322-330.
    Leal, Y., Velazquez, J., Hernandez, L., Swain, J. K., Rodríguez, A. R., Martínez, R., ... Carpio, Y. (2019). Promiscuous T cell epitopes boosts specific IgM immune response against a P0 peptide antigen from sea lice in different teleost species. Fish & Shellfish Immunology, 92, pp. 322-330. doi:10.1016/j.fsi.2019.06.018.
    Leal Y, et al. Promiscuous T Cell Epitopes Boosts Specific IgM Immune Response Against a P0 Peptide Antigen From Sea Lice in Different Teleost Species. Fish Shellfish Immunol. 2019 Jun 11;92:322-330. PubMed PMID: 31200071.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Promiscuous T cell epitopes boosts specific IgM immune response against a P0 peptide antigen from sea lice in different teleost species. AU - Leal,Yeny, AU - Velazquez,Janet, AU - Hernandez,Liz, AU - Swain,Jaya Kumari, AU - Rodríguez,Alianet Rodríguez, AU - Martínez,Rebeca, AU - García,Claudia, AU - Ramos,Yassel, AU - Estrada,Mario Pablo, AU - Carpio,Yamila, Y1 - 2019/06/11/ PY - 2019/02/01/received PY - 2019/06/07/revised PY - 2019/06/10/accepted PY - 2019/6/15/pubmed PY - 2019/6/15/medline PY - 2019/6/15/entrez KW - Epitopes KW - Immunoglobulin M KW - Lepeophtheirus salmonis KW - Peptide KW - Teleost KW - Vaccine SP - 322 EP - 330 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol. VL - 92 N2 - The development of vaccines employing conserved protein antigens, for instance ribosomal protein P0, has as disadvantage the high degree of identity between pathogen and host proteins due to possible induction of tolerance or auto antibodies in the host organism. To overcome this drawback, peptide-based vaccines have been designed with a proved high efficacy. The use of defined peptides as antigens has the problem that they are generally poor immunogenic unless coupled to a carrier protein. Several studies have established the potential for promiscuous T cell epitopes incorporated into chimeric peptides to enhance the immunogenicity in mammals. On the contrary, studies about the role of these epitopes on teleost immune system are scarce. Therefore, the main objective of our present study was to evaluate the potential of promiscuous T cell epitopes to boost specific IgM immune response in teleost fish against a peptide antigen. With this aim, we used a peptide of 35 amino acids from the ribosomal P0 protein of Lepeophtheirus salmonis, an important parasite in salmon aquaculture. We fused this peptide to the C-terminal of T cell epitopes from tetanus toxin and measles virus and produced the chimeric protein in Escherichia coli. Following vaccination, IgM antibody production was monitored in different immunization schemes in Tilapia, African catfish and Atlantic salmon. The results demonstrated for first time that the addition of T cell epitopes at the N-terminal of a target peptide increased IgM specific response in different teleost species, revealing the potential of this approach to develop peptide-based vaccines for aquaculture. The results are also of great importance in the context of vaccine development against sea lice using ribosomal protein P0 as antigen taking into account the key role of P0 in protein synthesis and other essential physiological processes. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/31200071/Promiscuous_T_cell_epitopes_boosts_specific_IgM_immune_response_against_a_P0_peptide_antigen_from_sea_lice_in_different_teleost_species L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(19)30665-5 DB - PRIME DP - Unbound Medicine ER -