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Overexpression of a trypanothione synthetase gene from Trypanosoma cruzi, TcTrys, confers enhanced tolerance to multiple abiotic stresses in rice.

Abstract

Plants are frequently exposed to variable environmental stresses that adversely affect plant growth, development and agricultural production. In this study, a trypanothione synthetase gene from Trypanosoma cruzi, TcTryS, was chemically synthesized and its roles in tolerance to multiple abiotic stresses were functionally characterized by generating transgenic rice overexpressing TcTryS. Overexpression of TcTryS in rice endows transgenic plants with hypersensitivity to ABA, hyposensitivity to NaCl- and mannitol-induced osmotic stress at the seed germination stage. TcTryS overexpression results in enhanced tolerance to drought, salt, cadmium, and 2,4,6-trichlorophenol stresses in transgenic rice, simultaneously supported by improved physiological traits. The TcTryS-overexpression plants also accumulated greater amounts of proline, less malondialdehyde and more transcripts of stress-related genes than wild-type plants under drought and salt stress conditions. In addition, TcTryS might play a positive role in maintaining chlorophyll content under 2,4,6-trichlorophenol stress. Histochemical staining assay showed that TcTryS renders transgenic plants better ROS-scavenging capability. All of these results suggest that TcTryS could function as a key regulator in modulation of abiotic stress tolerance in plant, and may have applications in the engineering of economically important crops.

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  • Authors+Show Affiliations

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    Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, PR China.

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    Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, PR China.

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    Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, PR China.

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    Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, PR China.

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    Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, PR China.

    ,

    Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, PR China.

    ,

    Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, PR China.

    ,

    Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, PR China.

    ,

    Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, PR China.

    ,

    Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, PR China.

    ,

    Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, PR China.

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    Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, PR China. Electronic address: rpeng_saas@163.com.

    Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, PR China. Electronic address: qhyao_saas@163.com.

    Source

    Gene 710: 2019 Aug 20 pg 279-290

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31200083

    Citation

    Li, Zhenjun, et al. "Overexpression of a Trypanothione Synthetase Gene From Trypanosoma Cruzi, TcTrys, Confers Enhanced Tolerance to Multiple Abiotic Stresses in Rice." Gene, vol. 710, 2019, pp. 279-290.
    Li Z, Fu X, Tian Y, et al. Overexpression of a trypanothione synthetase gene from Trypanosoma cruzi, TcTrys, confers enhanced tolerance to multiple abiotic stresses in rice. Gene. 2019;710:279-290.
    Li, Z., Fu, X., Tian, Y., Xu, J., Gao, J., Wang, B., ... Yao, Q. (2019). Overexpression of a trypanothione synthetase gene from Trypanosoma cruzi, TcTrys, confers enhanced tolerance to multiple abiotic stresses in rice. Gene, 710, pp. 279-290. doi:10.1016/j.gene.2019.06.018.
    Li Z, et al. Overexpression of a Trypanothione Synthetase Gene From Trypanosoma Cruzi, TcTrys, Confers Enhanced Tolerance to Multiple Abiotic Stresses in Rice. Gene. 2019 Aug 20;710:279-290. PubMed PMID: 31200083.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Overexpression of a trypanothione synthetase gene from Trypanosoma cruzi, TcTrys, confers enhanced tolerance to multiple abiotic stresses in rice. AU - Li,Zhenjun, AU - Fu,Xiaoyan, AU - Tian,Yongsheng, AU - Xu,Jing, AU - Gao,Jianjie, AU - Wang,Bo, AU - Han,Hongjuan, AU - Wang,Lijuan, AU - Zhang,Fujian, AU - Zhu,Yanman, AU - Huang,Younan, AU - Peng,Rihe, AU - Yao,Quanhong, Y1 - 2019/06/12/ PY - 2019/01/26/received PY - 2019/05/22/revised PY - 2019/06/10/accepted PY - 2019/6/15/pubmed PY - 2019/6/15/medline PY - 2019/6/15/entrez KW - Abiotic stresses KW - ROS KW - Rice KW - TcTryS KW - Trypanothione KW - Trypanothione synthetase SP - 279 EP - 290 JF - Gene JO - Gene VL - 710 N2 - Plants are frequently exposed to variable environmental stresses that adversely affect plant growth, development and agricultural production. In this study, a trypanothione synthetase gene from Trypanosoma cruzi, TcTryS, was chemically synthesized and its roles in tolerance to multiple abiotic stresses were functionally characterized by generating transgenic rice overexpressing TcTryS. Overexpression of TcTryS in rice endows transgenic plants with hypersensitivity to ABA, hyposensitivity to NaCl- and mannitol-induced osmotic stress at the seed germination stage. TcTryS overexpression results in enhanced tolerance to drought, salt, cadmium, and 2,4,6-trichlorophenol stresses in transgenic rice, simultaneously supported by improved physiological traits. The TcTryS-overexpression plants also accumulated greater amounts of proline, less malondialdehyde and more transcripts of stress-related genes than wild-type plants under drought and salt stress conditions. In addition, TcTryS might play a positive role in maintaining chlorophyll content under 2,4,6-trichlorophenol stress. Histochemical staining assay showed that TcTryS renders transgenic plants better ROS-scavenging capability. All of these results suggest that TcTryS could function as a key regulator in modulation of abiotic stress tolerance in plant, and may have applications in the engineering of economically important crops. SN - 1879-0038 UR - https://www.unboundmedicine.com/medline/citation/31200083/Overexpression_of_a_trypanothione_synthetase_gene_from_Trypanosoma_cruzi,_TcTrys,_confers_enhanced_tolerance_to_multiple_abiotic_stresses_in_rice L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(19)30578-5 DB - PRIME DP - Unbound Medicine ER -