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The complete genome of Rachiplusia nu nucleopolyhedrovirus (RanuNPV) and the identification of a baculoviral CPD-photolyase homolog.

Abstract

We described a novel baculovirus isolated from the polyphagous insect pest Rachiplusia nu. The virus presented pyramidal-shaped occlusion bodies (OBs) with singly-embed nucleocapsids and a dose mortality response of 6.9 × 103 OBs/ml to third-instar larvae of R. nu. The virus genome is 128,587 bp long with a G + C content of 37.9% and 134 predicted ORFs. The virus is an alphabaculovirus closely related to Trichoplusia ni single nucleopolyhedrovirus, Chrysodeixis chalcites nucleopolyhedrovirus, and Chrysodeixis includens single nucleopolyhedrovirus and may constitute a new species. Surprisingly, we found co-evolution among the related viruses and their hosts at species level. Besides, auxiliary genes with homologs in other baculoviruses were found, e.g. a CPD-photolyase. The gene seemed to be result of a single event of horizontal transfer from lepidopterans to alphabaculovirus, followed by a transference from alpha to betabaculovirus. The predicted protein appears to be an active enzyme that ensures likely DNA protection from sunlight.

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  • Authors+Show Affiliations

    ,

    Laboratory of Insect Virology, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil. Electronic address: luanabelotrentin@gmail.com.

    ,

    Laboratory of Insect Virology, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil. Electronic address: ethi_rozo@hotmail.com.

    ,

    Department of Microbiology, Londrina State University, Londrina, PR, 86057-970, Brazil. Electronic address: admilton@uel.br.

    ,

    Embrapa Soja, Londrina, PR, 86001-970, Brazil. Electronic address: drsgxx@gmail.com.

    ,

    Laboratory of Baculovirus, Cell Biology Department, University of Brasilia, Brasilia, DF, 70910-900, Brazil. Electronic address: bergmann@unb.br.

    Laboratory of Insect Virology, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil. Electronic address: daniel.araujo@ufsm.br.

    Source

    Virology 534: 2019 Aug pg 64-71

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31200103

    Citation

    Trentin, Luana Beló, et al. "The Complete Genome of Rachiplusia Nu Nucleopolyhedrovirus (RanuNPV) and the Identification of a Baculoviral CPD-photolyase Homolog." Virology, vol. 534, 2019, pp. 64-71.
    Trentin LB, Santos ER, Oliveira Junior AG, et al. The complete genome of Rachiplusia nu nucleopolyhedrovirus (RanuNPV) and the identification of a baculoviral CPD-photolyase homolog. Virology. 2019;534:64-71.
    Trentin, L. B., Santos, E. R., Oliveira Junior, A. G., Sosa-Gómez, D. R., Ribeiro, B. M., & Ardisson-Araújo, D. M. P. (2019). The complete genome of Rachiplusia nu nucleopolyhedrovirus (RanuNPV) and the identification of a baculoviral CPD-photolyase homolog. Virology, 534, pp. 64-71. doi:10.1016/j.virol.2019.05.019.
    Trentin LB, et al. The Complete Genome of Rachiplusia Nu Nucleopolyhedrovirus (RanuNPV) and the Identification of a Baculoviral CPD-photolyase Homolog. Virology. 2019;534:64-71. PubMed PMID: 31200103.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - The complete genome of Rachiplusia nu nucleopolyhedrovirus (RanuNPV) and the identification of a baculoviral CPD-photolyase homolog. AU - Trentin,Luana Beló, AU - Santos,Ethiane R, AU - Oliveira Junior,Admilton G, AU - Sosa-Gómez,Daniel R, AU - Ribeiro,Bergmann Morais, AU - Ardisson-Araújo,Daniel M P, Y1 - 2019/05/29/ PY - 2019/03/22/received PY - 2019/05/27/revised PY - 2019/05/28/accepted PY - 2019/6/15/pubmed PY - 2019/6/15/medline PY - 2019/6/15/entrez KW - Alphabaculovirus KW - Baculovirus genome KW - Evolution KW - Photolyase KW - Plusiinae KW - Rachiplusia nu nucleopolyhedrovirus SP - 64 EP - 71 JF - Virology JO - Virology VL - 534 N2 - We described a novel baculovirus isolated from the polyphagous insect pest Rachiplusia nu. The virus presented pyramidal-shaped occlusion bodies (OBs) with singly-embed nucleocapsids and a dose mortality response of 6.9 × 103 OBs/ml to third-instar larvae of R. nu. The virus genome is 128,587 bp long with a G + C content of 37.9% and 134 predicted ORFs. The virus is an alphabaculovirus closely related to Trichoplusia ni single nucleopolyhedrovirus, Chrysodeixis chalcites nucleopolyhedrovirus, and Chrysodeixis includens single nucleopolyhedrovirus and may constitute a new species. Surprisingly, we found co-evolution among the related viruses and their hosts at species level. Besides, auxiliary genes with homologs in other baculoviruses were found, e.g. a CPD-photolyase. The gene seemed to be result of a single event of horizontal transfer from lepidopterans to alphabaculovirus, followed by a transference from alpha to betabaculovirus. The predicted protein appears to be an active enzyme that ensures likely DNA protection from sunlight. SN - 1096-0341 UR - https://www.unboundmedicine.com/medline/citation/31200103/The_complete_genome_of_Rachiplusia_nu_nucleopolyhedrovirus_(RanuNPV)_and_the_identification_of_a_baculoviral_CPD-photolyase_homolog L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(19)30140-0 DB - PRIME DP - Unbound Medicine ER -