Tags

Type your tag names separated by a space and hit enter

Impaired Collateral Flow in Pial Arterioles of Aged Rats During Ischemic Stroke.

Abstract

Cerebral collateral circulation and age are critical factors in determining outcome from acute ischemic stroke. Aging may lead to rarefaction of cerebral collaterals, and thereby accelerate ischemic injury by reducing penumbral blood flow. Dynamic changes in pial collaterals after onset of cerebral ischemia may vary with age but have not been extensively studied. Here, laser speckle contrast imaging (LSCI) and two-photon laser scanning microscopy (TPLSM) were combined to monitor cerebral pial collaterals between the anterior cerebral artery (ACA) and the middle cerebral artery (MCA) in young adult and aged male Sprague Dawley rats during distal middle cerebral artery occlusion (dMCAo). Histological analysis showed that aged rats had significantly greater volumes of ischemic damage than young rats. LSCI showed that cerebral collateral perfusion declined over time after stroke in aged and young rats, and that this decline was significantly greater in aged rats. TPLSM demonstrated that pial arterioles narrowed faster after dMCAo in aged rats compared to young adult rats. Notably, while arteriole vessel narrowing was comparable 4.5 h after ischemic onset in aged and young adult rats, red blood cell velocity was stable in young adults but declined over time in aged rats. Overall, red blood cell flux through pial arterioles was significantly reduced at all time-points after 90 min post-dMCAo in aged rats relative to young adult rats. Thus, collateral failure is more severe in aged rats with significantly impaired pial collateral dynamics (reduced diameter, red blood cell velocity, and red blood cell flux) relative to young adult rats.

Authors+Show Affiliations

Neurochemical Research Unit, Department of Psychiatry, Faculty of Medicine and Dentistry, University of Alberta, 12-127 Clinical Sciences Building, Edmonton, AB, T6G 2R3, Canada. Neuroscience and Mental Health Institute, University of Alberta, Edmonton, AB, Canada. First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.Neurochemical Research Unit, Department of Psychiatry, Faculty of Medicine and Dentistry, University of Alberta, 12-127 Clinical Sciences Building, Edmonton, AB, T6G 2R3, Canada.Neuroscience and Mental Health Institute, University of Alberta, Edmonton, AB, Canada. Division of Neurology, Department of Medicine, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada.Neurochemical Research Unit, Department of Psychiatry, Faculty of Medicine and Dentistry, University of Alberta, 12-127 Clinical Sciences Building, Edmonton, AB, T6G 2R3, Canada. iwinship@ualberta.ca. Neuroscience and Mental Health Institute, University of Alberta, Edmonton, AB, Canada. iwinship@ualberta.ca.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31203565

Citation

Ma, Junqiang, et al. "Impaired Collateral Flow in Pial Arterioles of Aged Rats During Ischemic Stroke." Translational Stroke Research, 2019.
Ma J, Ma Y, Shuaib A, et al. Impaired Collateral Flow in Pial Arterioles of Aged Rats During Ischemic Stroke. Transl Stroke Res. 2019.
Ma, J., Ma, Y., Shuaib, A., & Winship, I. R. (2019). Impaired Collateral Flow in Pial Arterioles of Aged Rats During Ischemic Stroke. Translational Stroke Research, doi:10.1007/s12975-019-00710-1.
Ma J, et al. Impaired Collateral Flow in Pial Arterioles of Aged Rats During Ischemic Stroke. Transl Stroke Res. 2019 Jun 15; PubMed PMID: 31203565.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impaired Collateral Flow in Pial Arterioles of Aged Rats During Ischemic Stroke. AU - Ma,Junqiang, AU - Ma,Yonglie, AU - Shuaib,Ashfaq, AU - Winship,Ian R, Y1 - 2019/06/15/ PY - 2019/02/15/received PY - 2019/06/05/accepted PY - 2019/05/02/revised PY - 2019/6/17/entrez PY - 2019/6/17/pubmed PY - 2019/6/17/medline KW - Aging KW - Cerebral circulation KW - Collaterals KW - Ischemia JF - Translational stroke research JO - Transl Stroke Res N2 - Cerebral collateral circulation and age are critical factors in determining outcome from acute ischemic stroke. Aging may lead to rarefaction of cerebral collaterals, and thereby accelerate ischemic injury by reducing penumbral blood flow. Dynamic changes in pial collaterals after onset of cerebral ischemia may vary with age but have not been extensively studied. Here, laser speckle contrast imaging (LSCI) and two-photon laser scanning microscopy (TPLSM) were combined to monitor cerebral pial collaterals between the anterior cerebral artery (ACA) and the middle cerebral artery (MCA) in young adult and aged male Sprague Dawley rats during distal middle cerebral artery occlusion (dMCAo). Histological analysis showed that aged rats had significantly greater volumes of ischemic damage than young rats. LSCI showed that cerebral collateral perfusion declined over time after stroke in aged and young rats, and that this decline was significantly greater in aged rats. TPLSM demonstrated that pial arterioles narrowed faster after dMCAo in aged rats compared to young adult rats. Notably, while arteriole vessel narrowing was comparable 4.5 h after ischemic onset in aged and young adult rats, red blood cell velocity was stable in young adults but declined over time in aged rats. Overall, red blood cell flux through pial arterioles was significantly reduced at all time-points after 90 min post-dMCAo in aged rats relative to young adult rats. Thus, collateral failure is more severe in aged rats with significantly impaired pial collateral dynamics (reduced diameter, red blood cell velocity, and red blood cell flux) relative to young adult rats. SN - 1868-601X UR - https://www.unboundmedicine.com/medline/citation/31203565/Impaired_Collateral_Flow_in_Pial_Arterioles_of_Aged_Rats_During_Ischemic_Stroke DB - PRIME DP - Unbound Medicine ER -
Unbound Prime app for iOS iPhone iPadUnbound PubMed app for AndroidAlso Available:
Unbound MEDLINE
Unbound PubMed app for Windows