Tags

Type your tag names separated by a space and hit enter

Modulatory Effects of Single and Complex Vitamins on the In Vitro Growth of Murine Ovarian Follicles.
Tissue Eng Regen Med 2019; 16(3):275-283TE

Abstract

Background

Vitamin is a well-known co-factor for many metabolic processes and its roles in fertility and follicular growth have been studied. Vitamin supplementation is frequently achieved by daily ingestion in the form of a complex capsule. However, the role of single and complex vitamins in in vitro maturation of murine follicles is not fully elucidated.

Methods

In this study, we evaluated the effects of two forms of vitamins. Pure L-ascorbic acid, and multi-vitamin (vitamin C + vitamin B complex) was treated at two different concentrations (50 and 100 µg/ml), to pre-puberty murine follicles during in vitro maturation. To determine the specific stage of growth that is affected by treatment with vitamins, the vitamins were treated from day 0, 4, 9, and 13. Growth of each follicle was assessed by measuring diameters of whole expanded area and of the granulosa cells. Expression of follicular and oocyte growth-related genes and the effect of vitamin on the viability of follicles was assessed using senescence associated β-galactosidase staining.

Results

Treatment with vitamins promoted the in vitro growth of murine follicles and the upregulated the expression of granulosa cell- and oocyte-specific genes such as BMP15, Fsh receptor, and GDF9. The proliferation of the granulosa cells was enhanced by the treatment of vitamin. Fifty µg/ml concentration vitamin showed greater effects compared to higher concentration. The viability of in vitro grown follicles was also significantly improved in vitamin-treated follicles. The effects of single L-ascorbic acid and complex vitamin were not significantly different to those of day 4 and day 9 follicles. Vitamins promoted murine follicle development in vitro with different effects on specific growth stage.

Conclusion

Supplementation of vitamins during in vitro maturation of murine follicles is an efficient strategy for in vitro expansion of follicular cells. These results could be customized to the sophisticated culture of follicles retrieved from aged or cancer-survived female that contain smaller number of follicles with reduced potential to develop into mature follicles.

Authors+Show Affiliations

1Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-Gu, Seoul, 03080 Republic of Korea.2Department of Obstetrics and Gynecology, Korea University Guro Hospital, 148 Gurodong-ro, Guro-gu, Seoul, 08308 Republic of Korea.1Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-Gu, Seoul, 03080 Republic of Korea.3Biomedical Research Institute, Seoul National University Hospital, 101 Daehak-ro, Jongno-Gu, Seoul, 03080 Republic of Korea.1Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-Gu, Seoul, 03080 Republic of Korea.1Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-Gu, Seoul, 03080 Republic of Korea.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31205856

Citation

Kim, Yoon Young, et al. "Modulatory Effects of Single and Complex Vitamins On the in Vitro Growth of Murine Ovarian Follicles." Tissue Engineering and Regenerative Medicine, vol. 16, no. 3, 2019, pp. 275-283.
Kim YY, Kim YJ, Kim H, et al. Modulatory Effects of Single and Complex Vitamins on the In Vitro Growth of Murine Ovarian Follicles. Tissue Eng Regen Med. 2019;16(3):275-283.
Kim, Y. Y., Kim, Y. J., Kim, H., Kang, B. C., Ku, S. Y., & Suh, C. S. (2019). Modulatory Effects of Single and Complex Vitamins on the In Vitro Growth of Murine Ovarian Follicles. Tissue Engineering and Regenerative Medicine, 16(3), pp. 275-283. doi:10.1007/s13770-019-00188-w.
Kim YY, et al. Modulatory Effects of Single and Complex Vitamins On the in Vitro Growth of Murine Ovarian Follicles. Tissue Eng Regen Med. 2019;16(3):275-283. PubMed PMID: 31205856.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulatory Effects of Single and Complex Vitamins on the In Vitro Growth of Murine Ovarian Follicles. AU - Kim,Yoon Young, AU - Kim,Yong Jin, AU - Kim,Hoon, AU - Kang,Byeong Cheol, AU - Ku,Seung-Yup, AU - Suh,Chang Suk, Y1 - 2019/05/11/ PY - 2019/01/10/received PY - 2019/03/02/revised PY - 2019/03/04/accepted PY - 2020/05/11/pmc-release PY - 2019/6/18/entrez PY - 2019/6/18/pubmed PY - 2019/6/18/medline KW - In vitro maturation KW - L-ascorbic acid KW - Murine follicle KW - Vitamins SP - 275 EP - 283 JF - Tissue engineering and regenerative medicine JO - Tissue Eng Regen Med VL - 16 IS - 3 N2 - Background: Vitamin is a well-known co-factor for many metabolic processes and its roles in fertility and follicular growth have been studied. Vitamin supplementation is frequently achieved by daily ingestion in the form of a complex capsule. However, the role of single and complex vitamins in in vitro maturation of murine follicles is not fully elucidated. Methods: In this study, we evaluated the effects of two forms of vitamins. Pure L-ascorbic acid, and multi-vitamin (vitamin C + vitamin B complex) was treated at two different concentrations (50 and 100 µg/ml), to pre-puberty murine follicles during in vitro maturation. To determine the specific stage of growth that is affected by treatment with vitamins, the vitamins were treated from day 0, 4, 9, and 13. Growth of each follicle was assessed by measuring diameters of whole expanded area and of the granulosa cells. Expression of follicular and oocyte growth-related genes and the effect of vitamin on the viability of follicles was assessed using senescence associated β-galactosidase staining. Results: Treatment with vitamins promoted the in vitro growth of murine follicles and the upregulated the expression of granulosa cell- and oocyte-specific genes such as BMP15, Fsh receptor, and GDF9. The proliferation of the granulosa cells was enhanced by the treatment of vitamin. Fifty µg/ml concentration vitamin showed greater effects compared to higher concentration. The viability of in vitro grown follicles was also significantly improved in vitamin-treated follicles. The effects of single L-ascorbic acid and complex vitamin were not significantly different to those of day 4 and day 9 follicles. Vitamins promoted murine follicle development in vitro with different effects on specific growth stage. Conclusion: Supplementation of vitamins during in vitro maturation of murine follicles is an efficient strategy for in vitro expansion of follicular cells. These results could be customized to the sophisticated culture of follicles retrieved from aged or cancer-survived female that contain smaller number of follicles with reduced potential to develop into mature follicles. SN - 2212-5469 UR - https://www.unboundmedicine.com/medline/citation/31205856/Modulatory_Effects_of_Single_and_Complex_Vitamins_on_the_In_Vitro_Growth_of_Murine_Ovarian_Follicles DB - PRIME DP - Unbound Medicine ER -
Unbound Prime app for iOS iPhone iPadUnbound PubMed app for AndroidAlso Available:
Unbound MEDLINE
Unbound PubMed app for Windows