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Analysis of Young Shoots of 'Anji Baicha' (Camellia sinensis) at Three Developmental Stages Using Nontargeted LC-MS-Based Metabolomics.
J Food Sci. 2019 Jul; 84(7):1746-1757.JF

Abstract

'Anji Baicha' (Camellia sinensis) is a low-temperature-sensitive tea variety. During the development of young shoots, the leaves of 'Anji Baicha' exhibit periodic albinism. The quality of 'Anji Baicha' is closely related to the color of the fresh leaves, with whiter leaves affording a higher amino acid content and superior sensory quality after processing. However, the metabolic mechanism of its quality formation is still unclear. In this study, we analyzed the metabolomic changes of young shoots of 'Anji Baicha' and screened for metabolic markers that may be involved in the periodic albinism. Positive- and negative-mode UPLC-QTOF-MS was applied to the metabolomic analysis of young leaves of 'Anji Baicha' during three developmental stages (i.e., the pre-albescent, albescent, and regreening stages). The results revealed significant differences in the metabolic profiles of the young leaves at the three stages. The differential metabolites were mainly related to the pathways of flavonoid, phenylpropanoid, and amino acid biosynthesis. The concentrations of several amino acids (primarily l-theanine, l-glutamate, N2 -acetyl-l-ornithine, l-aspartic acid, d-proline, l-glutamine, l-leucine, and pyroglutamic acid) and 12-OPDA were significantly higher in the albescent stage. In contrast, during the albescent stages, the concentrations of several carbohydrates (d-fructose, β-d-galactopyranose, 3-O-fucopyranosyl-2-acetamido-2-deoxyglucopyranose, galactose-β-1, 4-xylose acetyl-maltose, and 2-fucosyllactose) were significantly lower. Moreover, catechins (mainly epigallocatechin and catechin derivatives), dimeric catechins (primarily proanthocyanidins), and flavonol and flavonol/flavone glycosides (mainly kaempferol, myricetin, quercetin, cyanidin, and delphinidin glycosides) were detected at the highest levels in the regreening or pre-albescent stages. The obtained results enhance the current understanding of the metabolic mechanisms of periodic albinism and quality development formation in 'Anji Baicha'. PRACTICAL APPLICATION: The obtained results not only provide information regarding differential metabolites but also advance the understanding of the mechanism of periodic albinism in 'Anji Baicha' at the metabolite level and open up new possibilities for the genetic improvement of tea cultivars.

Authors+Show Affiliations

College of Life Science and Technology, Central South Univ. of Forestry and Technology, Changsha, Hunan, 410004, China. Hunan Provincial Base for Scientific and Technological Innovation Cooperation, Changsha, 410004, China. Hunan Provincial Key Lab. of Crop Germplasm Innovation and Utilization, Changsha, 410128, China. Key Lab. of Tea Science, Ministry of Education, Hunan Agricultural Univ., Changsha, Hunan, 410128, China.Key Lab. of Tea Science, Ministry of Education, Hunan Agricultural Univ., Changsha, Hunan, 410128, China.College of Life Science and Technology, Central South Univ. of Forestry and Technology, Changsha, Hunan, 410004, China. Hunan Provincial Base for Scientific and Technological Innovation Cooperation, Changsha, 410004, China. Hunan Provincial Key Lab. of Crop Germplasm Innovation and Utilization, Changsha, 410128, China.Key Lab. of Tea Science, Ministry of Education, Hunan Agricultural Univ., Changsha, Hunan, 410128, China. Natl. Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha, Hunan, 410128, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31206686

Citation

Zeng, Chaozhen, et al. "Analysis of Young Shoots of 'Anji Baicha' (Camellia Sinensis) at Three Developmental Stages Using Nontargeted LC-MS-Based Metabolomics." Journal of Food Science, vol. 84, no. 7, 2019, pp. 1746-1757.
Zeng C, Lin H, Liu Z, et al. Analysis of Young Shoots of 'Anji Baicha' (Camellia sinensis) at Three Developmental Stages Using Nontargeted LC-MS-Based Metabolomics. J Food Sci. 2019;84(7):1746-1757.
Zeng, C., Lin, H., Liu, Z., & Liu, Z. (2019). Analysis of Young Shoots of 'Anji Baicha' (Camellia sinensis) at Three Developmental Stages Using Nontargeted LC-MS-Based Metabolomics. Journal of Food Science, 84(7), 1746-1757. https://doi.org/10.1111/1750-3841.14657
Zeng C, et al. Analysis of Young Shoots of 'Anji Baicha' (Camellia Sinensis) at Three Developmental Stages Using Nontargeted LC-MS-Based Metabolomics. J Food Sci. 2019;84(7):1746-1757. PubMed PMID: 31206686.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of Young Shoots of 'Anji Baicha' (Camellia sinensis) at Three Developmental Stages Using Nontargeted LC-MS-Based Metabolomics. AU - Zeng,Chaozhen, AU - Lin,Haiyan, AU - Liu,Zhixiang, AU - Liu,Zhonghua, Y1 - 2019/06/17/ PY - 2019/01/09/received PY - 2019/04/08/revised PY - 2019/04/23/accepted PY - 2019/6/18/pubmed PY - 2019/10/15/medline PY - 2019/6/18/entrez KW - LC-MS KW - chemical components KW - developmental stages KW - metabolomics KW - ‘Anji Baicha’ (Camellia sinensis) SP - 1746 EP - 1757 JF - Journal of food science JO - J Food Sci VL - 84 IS - 7 N2 - 'Anji Baicha' (Camellia sinensis) is a low-temperature-sensitive tea variety. During the development of young shoots, the leaves of 'Anji Baicha' exhibit periodic albinism. The quality of 'Anji Baicha' is closely related to the color of the fresh leaves, with whiter leaves affording a higher amino acid content and superior sensory quality after processing. However, the metabolic mechanism of its quality formation is still unclear. In this study, we analyzed the metabolomic changes of young shoots of 'Anji Baicha' and screened for metabolic markers that may be involved in the periodic albinism. Positive- and negative-mode UPLC-QTOF-MS was applied to the metabolomic analysis of young leaves of 'Anji Baicha' during three developmental stages (i.e., the pre-albescent, albescent, and regreening stages). The results revealed significant differences in the metabolic profiles of the young leaves at the three stages. The differential metabolites were mainly related to the pathways of flavonoid, phenylpropanoid, and amino acid biosynthesis. The concentrations of several amino acids (primarily l-theanine, l-glutamate, N2 -acetyl-l-ornithine, l-aspartic acid, d-proline, l-glutamine, l-leucine, and pyroglutamic acid) and 12-OPDA were significantly higher in the albescent stage. In contrast, during the albescent stages, the concentrations of several carbohydrates (d-fructose, β-d-galactopyranose, 3-O-fucopyranosyl-2-acetamido-2-deoxyglucopyranose, galactose-β-1, 4-xylose acetyl-maltose, and 2-fucosyllactose) were significantly lower. Moreover, catechins (mainly epigallocatechin and catechin derivatives), dimeric catechins (primarily proanthocyanidins), and flavonol and flavonol/flavone glycosides (mainly kaempferol, myricetin, quercetin, cyanidin, and delphinidin glycosides) were detected at the highest levels in the regreening or pre-albescent stages. The obtained results enhance the current understanding of the metabolic mechanisms of periodic albinism and quality development formation in 'Anji Baicha'. PRACTICAL APPLICATION: The obtained results not only provide information regarding differential metabolites but also advance the understanding of the mechanism of periodic albinism in 'Anji Baicha' at the metabolite level and open up new possibilities for the genetic improvement of tea cultivars. SN - 1750-3841 UR - https://www.unboundmedicine.com/medline/citation/31206686/Analysis_of_Young_Shoots_of_'Anji_Baicha'__Camellia_sinensis__at_Three_Developmental_Stages_Using_Nontargeted_LC_MS_Based_Metabolomics_ L2 - https://doi.org/10.1111/1750-3841.14657 DB - PRIME DP - Unbound Medicine ER -